1. bookVolume 67 (2018): Issue 1 (February 2018)
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
access type Open Access

Genetic diversity and structure among natural populations of Mytilaria laosensis (Hamamelidaceae) revealed by microsatellite markers

Published Online: 05 Oct 2018
Page range: 93 - 98
Journal Details
License
Format
Journal
eISSN
2509-8934
First Published
22 Feb 2016
Publication timeframe
1 time per year
Languages
English
Abstract

Mytilaria laosensis is a fast-growing tropical broadleaf tree that is extensively used for wood production and has significant ecological benefits. To investigate the genetic diversity and population structure of M. laosensis, eight major natural popu­lations in China were analyzed by using simple sequence repeat (SSR) markers. A total of 88 microsatellite-containing fragments were obtained by the method of magnetic bead enrichment, among which 26 pairs of SSR primers were scree­ned out and used to generate a total of 190 alleles among 152 individuals. The average of observed number of alleles, Shannon’s information index and polymorphism information content per locus were 18.3, 1.1577 and 0.7759, respectively, implying a high level of genetic diversity in M. laosensis popu­lations. The variation within populations accounted for 81.74 % of total variation based on analysis of molecular variance. Clus­ter analysis divided the eight populations into four groups, among which five populations from the southern parts of Guangxi province were classified as one major group. Mantel test showed that there was highly significant correlation bet­ween Euclidean genetic distance and geographic distance, suggesting that geographic isolation contribute to the high genetic diversity of M. laosensis. Together, these could provide support for the feasibility of exploration and utilization of M. laosensis in subtropical areas of East Asia including Jiangxi, Hunan and Fujian province of China.

Keywords

Adams WT, Strauss, SH, Copes DL, Griffin AR (1990) Population Genetics of For­est Trees. Proceedings of the International Symposium on Population Ge­netics of Forest Trees Corvallis, Oregon, U.S.A.Search in Google Scholar

Bacilieri R, Lacombe T, Le Cunff L, Di Vecchi-Staraz M, Laucou V, Genna B, Péros JP, This P, Boursiquot JM (2013) Genetic structure in cultivated grapevines is linked to geography and human selection. BMC Plant Biol. 13: 25. https://doi.org/10.1186/1471-2229-13-2510.1186/1471-2229-13-25Open DOISearch in Google Scholar

Bassam BJ, Caetano-Anollés G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem. 196: 80-83. https://doi.org/10.1016/0003-2697(91)90120-i10.1016/0003-2697(91)90120-iOpen DOISearch in Google Scholar

Chen L, Zeng J, Jia HY, Zeng J, Guo WF, Cai DX (2012) Growth and nutrient up­take dynamics of Mytilaria laosensis seedlings under exponential and con­ventional fertilizations. Soil Sci Plant Nutr. 58: 618-626. https://doi.org/10.1080/00380768.2012.70887910.1080/00380768.2012.708879Open DOISearch in Google Scholar

Clarke KR, Gorley RN (2001) PRIMER v5: User Manual/Tutorial. PRIMER-E Ltd, Plymouth.Search in Google Scholar

Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of indi­viduals using the software STRUCTURE: a simulation study. Mol Ecol. 14: 2611-2620. https://doi.org/10.1111/j.1365-294x.2005.02553.x10.1111/j.1365-294x.2005.02553.x15969739Open DOISearch in Google Scholar

Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated soft­ware package for population genetics data analysis. Evol Bioinform. 1: 47- 50. https://doi.org/10.1177/11769343050010000310.1177/117693430500100003Search in Google Scholar

Fan L, Zheng H, Milne RI, Zhang L, Mao K (2018) Strong population bottleneck and repeated demographic expansions of Populus adenopoda (Salicaceae) in subtropical China. Ann Bot. 121(4): 665-679. https://doi.org/10.1093/aob/mcx19810.1093/aob/mcx198585302829324975Open DOISearch in Google Scholar

Gong W, Liu WZ, Gu L, Kaneko S, Koch M. A, Zhang DX (2016) From glacial refu­gia to wide distribution range: demographic expansion of Loropetalum chinense (Hamamelidaceae) in Chinese subtropical evergreen broadleaved forest. Org Divers Evol. 16(1): 23-38. https://doi.org/10.1007/s13127-015-0252-410.1007/s13127-015-0252-4Open DOISearch in Google Scholar

Guo WF, Cai DX, Jia HY, Li YX, Lu ZF (2006) Growth laws of Mytilaria laosensis plantation. For Res. 19: 585-589.Search in Google Scholar

Huang ZQ, Yu ZP, Wang MH (2014) Environmental controls and the influence of tree species on temporal variation in soil respiration in subtropical China. Plant Soil. 382: 75-87. https://doi.org/10.1007/s11104-014-2141-610.1007/s11104-014-2141-6Open DOISearch in Google Scholar

Huang Z T, Wang S F, Jiang Y M, Mo J X (2009) Exploitation and utilization pros­pects of eximious native tree species Mytilaria laosensis. J Guangxi Agric Sci. 40: 1220-1223.Search in Google Scholar

Li F, Gan S (2011) An optimised protocol for fluorescent-dUTP based SSR geno­typing and its application to genetic mapping in Eucalyptus. Silvae Genet. 60(1): 18-25. https://doi.org/10.1515/sg-2011-000310.1515/sg-2011-0003Open DOISearch in Google Scholar

Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for ge­netic marker analysis. Bioinformatics. 21: 2128-2129. https://doi.org/10.1093/bioinformatics/bti28210.1093/bioinformatics/bti28215705655Open DOISearch in Google Scholar

Miller MP (1999) Tools for population genetic analyses (TFPGA): a windows ™ program for the analysis of allozyme and molecular population genetic data. http://www.marksgeneticsoftware.net/tfpga.htm.Search in Google Scholar

Ming AG, Jia HY, Tao Y, Lu LH, Su JM (2012) Biomass and its allocation in 28-year-old Mytilaria laosensis plantation in southwest Guangxi. Chinese J Ecol. 31: 1050-1056.Search in Google Scholar

Ming AG, Jia HY, Zhao JL, Tao Y, Li YF (2016) Above- and below-ground carbon stocks in an indigenous tree (Mytilaria laosensis) plantation chronose­quence in subtropical China. Plos One 9: e109730. https://doi.org/10.1371/journal.pone.010973010.1371/journal.pone.0109730420876025343446Search in Google Scholar

Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89: 583-90.10.1093/genetics/89.3.583121385517248844Search in Google Scholar

Olango TM, Tesfaye B, Pagnotta MA, Pè ME, Catellani M (2015) Development of SSR markers and genetic diversity analysis in enset (Ensete ventricosum (Welw.) Cheesman), an orphan food security crop from Southern Ethiopia. BMC Genet. 16: 98. https://doi.org/10.1186/s12863-015-0250-810.1186/s12863-015-0250-8452439426243662Open DOISearch in Google Scholar

Peakall ROD, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol ecol notes. 6: 288-295. https://doi.org/10.1111/j.1471-8286.2005.01155.x10.1111/j.1471-8286.2005.01155.xOpen DOISearch in Google Scholar

Peng JQ, Cao FX, Xu RX (2012) Genetic diversity of the Mytilaria laosensis in Guangxi detected by ISSR markers. J Nat Sci Hunan Norm Uni. 35: 61-65.Search in Google Scholar

Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed. 2(3): 225-238. https://doi.org/10.1007/bf0056420010.1007/bf00564200Open DOISearch in Google Scholar

Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945-959.10.1093/genetics/155.2.945146109610835412Search in Google Scholar

Rosenberg NA (2004) Distruct: a program for the graphical display of population structure. Mol Ecol Notes. 4: 137-138. https://doi.org/10.1046/j.1471-8286.2003.00566.x10.1046/j.1471-8286.2003.00566.xSearch in Google Scholar

Shi MM, Michalski SG, Welk E, Chen XY, Durka W (2014) Phylogeography of a widespread Asian subtropical tree: genetic east-west differentiation and climate envelope modelling suggest multiple glacial refugia. J Biogeogr. 41: 1710-1720. https://doi.org/10.1111/jbi.1232210.1111/jbi.12322Open DOISearch in Google Scholar

Sokal R (1979) Testing statistical significance of geographic variation patterns. Syst Zool. 28: 227-232. https://doi.org/10.1093/sysbio/28.2.227a10.1093/sysbio/28.2.227aOpen DOISearch in Google Scholar

Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary ge­netics analysis (MEGA) software version 4.0. Mol Biol Evol. 24: 1596-1599. https://doi.org/10.1093/molbev/msm09210.1093/molbev/msm09217488738Search in Google Scholar

Wu KS, Tanksley SD (1993) Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol Gen Genet. 241(1-2):225-35. https://doi.org/10.1007/bf0028022010.1007/bf00280220Open DOISearch in Google Scholar

Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Molecular Ecology, 11(1): 1-16 https://doi.org/10.1046/j.0962-1083.2001.01418.x10.1046/j.0962-1083.2001.01418.x11903900Open DOISearch in Google Scholar

Zehdi-Azouzi S, Cherif E, Moussouni S, et al (2015) Genetic structure of the date palm (Phoenix dactylifera) in the Old World reveals a strong differentiation between eastern and western populations. Ann Bot. 116: 101-112. https://doi.org/10.1093/aob/mcv06810.1093/aob/mcv068447975526113618Open DOISearch in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo