1. bookVolume 30 (2022): Issue 4 (October 2022)
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
Open Access

Protocol and Matters for Consideration for the Treatment of Polymerase Chain Reaction Contamination in Next-Generation Sequencing Laboratories

Published Online: 20 Oct 2022
Volume & Issue: Volume 30 (2022) - Issue 4 (October 2022)
Page range: 467 - 475
Received: 15 Jun 2022
Accepted: 14 Sep 2022
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English

1. Champlot S, Berthelot C, Pruvost M, Bennett EA, Grange T, Geigl EM. An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications. PLoS One. 2010 Sep 28;5(9):e13042. DOI: 10.1371/journal.pone.001304210.1371/journal.pone.0013042294691720927390 Search in Google Scholar

2. Banasik M, Stanislawska-Sachadyn A, Sachadyn P. A simple modification of PCR thermal profile applied to evade persisting contamination. J Applg Genet. 2016;57(3):409-15. DOI: 10.1007/s13353-015-0336-z10.1007/s13353-015-0336-z496343526811932 Search in Google Scholar

3. Mănescu IB, Pavelea O, Dobreanu M. Troubleshootings in RNA extraction - back to basic chemistry. Rev Romana Med Lab. 2020;28(4):441-5. DOI: 10.2478/rrlm-2020-003410.2478/rrlm-2020-0034 Search in Google Scholar

4. Wu Y, Wu J, Zhang Z, Cheng C. DNA decontamination methods for internal quality management in clinical PCR laboratories. J Clin Lab Anal. 2018 Mar;32(3):e22290. DOI: 10.1002/jcla.2229010.1002/jcla.22290681690528665527 Search in Google Scholar

5. Fischer M, Renevey N, Thur B, Hoffmann D, Beer M, Hoffmann B. Efficacy Assessment of Nucleic Acid De-contamination Reagents Used in Molecular Diagnostic Laboratories. PLoS One. 2016;11(7):e159274. DOI: 10.1371/journal.pone.015927410.1371/journal.pone.0159274494365327410228 Search in Google Scholar

6. Taylor AV, Baker N, Hulsey M, Bennett CC, Meiners M, Gonzales BA. Evaluating containment effectiveness of A2 and B2 biological safety cabinets. Am J Health Syst Pharm. 2019;76(9):599-607. DOI: 10.1093/ajhp/zxz03010.1093/ajhp/zxz03031361829 Search in Google Scholar

7. Andersson D, Svec D, Pedersen C, Henriksen JR, Stahl-berg A. Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons. Int J Mol Sci. 2018 Oct; 19(10): 3185. DOI: 10.3390/ijms1910318510.3390/ijms19103185621410030332749 Search in Google Scholar

8. Setlow B, Parish S, Zhang P, Li YQ, Neely WC, Setlow P. Mechanism of killing of spores of Bacillus anthracis in a high-temperature gas environment, and analysis of DNA damage generated by various decontamination treatments of spores of Bacillus anthracis, Bacillus subtilis and Bacillus thuringiensis. J Appl Microbiol. 2014 Apr;116(4):805-14. DOI: 10.1111/jam.1242110.1111/jam.1242124344920 Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo