<abstract xmlns="http://www.w3.org/1999/xhtml">

<bold>Introduction</bold>: Crinum latifolium is a natural plant showing the anti-inflammatory effects. We aimed to evaluate the effects of Crinum latifolium extract on proinflammatory cytokine production and maturation of early and late endosomes in the lipopolysaccharide (LPS)-induced human macrophages at light microscopic and biochemical levels.
<bold>Material and Methods</bold>: The effects of Crinum latifolium extract in human macrophages stimulated with LPS was evaluated to optic densities of the lysosome-associated membrane protein-1 (LAMP-1) and early endo-some antigen 1 (EEA1) by immunohistochemistry staining and to level of the proinflammatory cytokine interleukin (IL)-6 by enzyme-linked immunosorbent assay (ELISA).
<bold>Results</bold>: Crinum latifolium group exhibited a significant decreased in the levels of IL-6 in the supernatant as compared to the LPS group for 2 and 72 hours (p&lt;0.0001). Crinum latifolium group exhibited a significant increased in the optic density of the EEA1 and LAMP-1 in coverslips as compared to the control and lipopolysaccharide groups after 2 and 72 hours (p&lt;0.0001).
<bold>Conclusions</bold>: Crinum latifolium may a therapeutic plant in the inflammatory diseases, such as sepsis, through anti-inflammatory effects, such as decrease in production of the proinflammatory cytokine and increase in maturations of the early and late endosomes in macrophages.
</abstract>

Introduction: Crinum latifolium is a natural plant showing the anti-inflammatory effects. We aimed to evaluate the effects of Crinum latifolium extract on proinflammatory cytokine production and maturation of early and late endosomes in the lipopolysaccharide (LPS)-induced human macrophages at light microscopic and biochemical levels.
Material and Methods: The effects of Crinum latifolium extract in human macrophages stimulated with LPS was evaluated to optic densities of the lysosome-associated membrane protein-1 (LAMP-1) and early endo-some antigen 1 (EEA1) by immunohistochemistry staining and to level of the proinflammatory cytokine interleukin (IL)-6 by enzyme-linked immunosorbent assay (ELISA).
Results: Crinum latifolium group exhibited a significant decreased in the levels of IL-6 in the supernatant as compared to the LPS group for 2 and 72 hours (p<0.0001). Crinum latifolium group exhibited a significant increased in the optic density of the EEA1 and LAMP-1 in coverslips as compared to the control and lipopolysaccharide groups after 2 and 72 hours (p<0.0001).
Conclusions: Crinum latifolium may a therapeutic plant in the inflammatory diseases, such as sepsis, through anti-inflammatory effects, such as decrease in production of the proinflammatory cytokine and increase in maturations of the early and late endosomes in macrophages.

Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages

Department of Histology and Embryology, Faculty of Medicine

Department of Medical Microbiology, Faculty of Medicine

Department of Physiology, Faculty of Medicine

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution 4.0 International License.

Introduction: Crinum latifolium is a natural plant showing the anti-inflammatory effects. We aimed to evaluate the effects of Crinum latifolium extract on...

Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages

Published Online: May 09, 2022

Page range: 183 - 190

Received: Nov 20, 2021

Accepted: Mar 21, 2022

DOI: https://doi.org/10.2478/rrlm-2022-0015

Keywords
inflammation, macrophage, interleukin-6, Crinum latifolium extract, endosomes

© 2022 Sibel Köktürk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages

Published Online: May 09, 2022

Page range: 183 - 190

Received: Nov 20, 2021

Accepted: Mar 21, 2022

DOI: https://doi.org/10.2478/rrlm-2022-0015

Keywordsinflammation, macrophage, interleukin-6, Crinum latifolium extract, endosomes

© 2022 Sibel Köktürk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
inflammation, macrophage, interleukin-6, Crinum latifolium extract, endosomes