[1. Jemal A, Siegel R, Xu J, Ward E. Cancer statistics, 2010. CA Cancer J Clin 2010 Sep-Oct;60(5):277-300. DOI: 10.3322/caac.2007310.3322/caac.2007320610543]Search in Google Scholar
[2. DeNardo DG, Andreu P, Coussens LM. Interactions between lymphocytes and myeloid cells regulate pro- versus anti-tumor immunity. Cancer Metastasis Rev. 2010 Jun;29(2):309-16. DOI: 10.1007/s10555-010-9223-610.1007/s10555-010-9223-6286563520405169]Search in Google Scholar
[3. Rakha EA, Reis-Filho JS, Ellis IO. Combinatorial biomarker expression in breast cancer. Breast Cancer Res Treat. 2010 Apr;120(2):293-308. DOI: 10.1007/ s10549-010-0746-x10.1007/s10549-010-0746-x20107892]Search in Google Scholar
[4. Jochems C, Schlom J. Tumor-infiltrating immune cells and prognosis: the potential link between conventional cancer therapy and immunity. Exp Biol Med (Maywood). 2011 May;236(5):567-79. DOI: 10.1258/ ebm.2011.01100710.1258/ebm.2011.011007322926121486861]Search in Google Scholar
[5. Fridman WH, Pagès F, Sautès-Fridman C, Galon J. The immune contexture in human tumours: impact on clinical outcome. Nat Rev Cancer. 2012 Mar;12(4):298-306. DOI: 10.1038/nrc324510.1038/nrc324522419253]Search in Google Scholar
[6. Gerner MY, Kastenmuller W, Ifrim I, Kabat J, Germain RN. Histo-cytometry: a method for highly multiplex quantitative tissue imaging analysis applied to dendritic cell subset microanatomy in lymph nodes. Immunity. 2012 Aug;37(2):364-76. DOI: 10.1016/j.immuni. 2012.07.011]Search in Google Scholar
[7. Ascierto PA, Capone M, Urba WJ, Bifulco CB, Botti G, Lugli A, et al. The additional facet of immunoscore: immunoprofiling as a possible predictive tool for cancer treatment. J Transl Med. 2013 Mar;11:54. DOI: 10.1186/1479-5876-11-5410.1186/1479-5876-11-54360822523452415]Search in Google Scholar
[8. Galon J, Pages F, Marincola FM, Thurin M, Trinchieri G, Fox BA, et al. The immune scor as a new possible approach for the classification of cancer. J Transl Med 2012 Jan;10:1. DOI: 10.1186/1479-5876-10-110.1186/1479-5876-10-1326936822214470]Search in Google Scholar
[9. Al-Kofahi Y, Lassoued W, Grama K, Nath SK, Zhu J, Oueslati R, et al. Cell-based quantification of molecular biomarkers in histopathology specimens. Histopathology 2011 Jul;59(1):40-54. DOI: 10.1111/j.1365-2559.2011.03878.x10.1111/j.1365-2559.2011.03878.x314209521771025]Search in Google Scholar
[10. Eiró N, Pidal I, Fernandez-Garcia B, Junquera S, Lamelas ML, del Casar JM, et al. Impact of CD68/ (CD3+CD20) ratio at the invasive front of primary tumors on distant metastasis development in breast cancer. PLoS One. 2012; 7(12): e52796. DOI: 10.1371/ journal.pone.005279610.1371/journal.pone.0052796353050823300781]Search in Google Scholar
[11. Chang H, Yang Q, Parvin B. Segmentation of heterogeneous blob objects through voting and level set for mulation. Pattern Recognit Lett. 2007;28(13):1781-7. DOI: 10.1016/j.patrec.2007.05.00810.1016/j.patrec.2007.05.008274732519774202]Search in Google Scholar
[12. Lin G, Chawla MK, Olson K, Guzowski JF, Barnes CA, Roysam B. Hierarchical, model-based merging of multiple fragments for improved three-dimensional segmentation of nuclei. Cytometry A. 2005;63(1):20-33. DOI: 10.1002/cyto.a.2009910.1002/cyto.a.20099]Search in Google Scholar
[13. De Solorzano CO, Malladi R, Lelievre SA, Lockett SJ. Segmentation of nuclei and cells using membrane related protein markers. J Microsc. 2001 Mar;201(Pt 3):404-1. DOI: 10.1046/j.1365-2818.2001.00854.x10.1046/j.1365-2818.2001.00854.x]Search in Google Scholar
[14. Ancin H, Roysam B, Dufresne TE, Chestnut MM, Ridder GM, Szarowski DH, et al. Advances in automated 3-D image analyses of cell populations imaged by confocal microscopy. Cytometry. 1996 Nov;25(3):221-34. DOI: 10.1002/(SICI)1097-0320(19961101)25:3<221::AIDCYTO3> 3.3.CO;2-O DOI: 10.1002/(SICI)1097-0320(19961101)25:3<221::AID-CYTO3>3.0.CO;2-I10.1002/(SICI)1097-0320(19961101)25:3<221::AID-CYTO3>3.0.CO;2-I]Search in Google Scholar
[15. Căruntu ID, Covic A. Renal corpuscle morphometry with increased reliability and high level of automation. Pathol Res Pract. 2007;203:9-20. DOI: 10.1016/j. prp.2006.06.002]Search in Google Scholar
[16. Galon J, Pagès F, Marincola FM, Thurin M, Trinchieri G, Fox BA, et al. The immune score as a new possible approach for the classification of cancer. J Transl Med. 2012 Jan;10:1. DOI: 10.1186/1479-5876-10-110.1186/1479-5876-10-1]Search in Google Scholar
[17. Mahmoud SM, Paish EC, Powe DG, Macmillan RD, Grainge MJ, Lee AH, et al. Tumor-infiltrating CD8+ lymphocytes predict clinical outcome in breast cancer. J Clin Oncol. 2011 May;29(15):1949-55. DOI: 10.1200/ JCO.2010.30.503710.1200/JCO.2010.30.5037]Search in Google Scholar
[18. Dutta S, Going JJ, Crumley AB, Mohammed Z, Orange C, Edwards J, et al. The relationship between tumour necrosis, tumour proliferation, local and systemic inflammation, microvessel density and survival in patients undergoing potentially curative resection of oesophageal adenocarcinoma. Br J Cancer. 2012 Feb;106(4):702-10. DOI: 10.1038/bjc.2011.61010.1038/bjc.2011.610]Search in Google Scholar
[19. Mahmoud SM, Lee AH, Paish EC, Macmillan RD, Ellis IO, Green AR. The prognostic significance of B lymphocytes in invasive carcinoma of the breast. Breast Cancer Res Treat. 2012 Apr;132(2):545-53. DOI: 10.1007/s10549-011-1620-110.1007/s10549-011-1620-1]Search in Google Scholar
[20. Turbin DA, Leung S, Cheang MC, Kennecke HA, Montgomery KD, McKinney S, et al. Automated quantitative analysis of estrogen receptor expression in breast carcinoma does not differ from expert pathologist scoring: a tissue microarray study of 3,484 cases. Breast Cancer Res Treat. 2008 Aug;110(3):417-26. DOI: 10.1007/s10549-007-9736-z10.1007/s10549-007-9736-z]Search in Google Scholar
[21. Budczies J, Klauschen F, Sinn BV, Győrffy B, Schmitt WD, Darb-Esfahani S, et al. Cutoff Finder: a comprehensive and straightforward Web application enabling rapid biomarker cutoff optimization. PLoS One. 2012;7(12):e51862. DOI: 10.1371/journal. pone.0051862 ]Search in Google Scholar