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Radiology and Oncology
Volume 57 (2023): Issue 1 (March 2023)
Open Access
Pancreatic islets implanted in an irreversible electroporation generated extracellular matrix in the liver
Yanfang Zhang
Yanfang Zhang
,
Yanpeng Lv
Yanpeng Lv
,
Yunlong Wang
Yunlong Wang
,
Tammy T Chang
Tammy T Chang
and
Boris Rubinsky
Boris Rubinsky
| Jan 19, 2023
Radiology and Oncology
Volume 57 (2023): Issue 1 (March 2023)
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Article Category:
Research Article
Published Online:
Jan 19, 2023
Page range:
51 - 58
Received:
Nov 05, 2022
Accepted:
Nov 24, 2022
DOI:
https://doi.org/10.2478/raon-2023-0006
© 2023 Yanfang Zhang, Yanpeng Lv, Yunlong Wang, Tammy T Chang, Boris Rubinsky, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Figure 1
Photograph of a clamp electroporation electrode on a liver lobe.
Figure 2
Photograph of the pancreatic islets used in this study stained by dithizone (DTZ). Arrows point to the few unstained exocrine cells left after digestion by collagenase.
Figure 3
An illustration showing the implantation site in the liver. The dark area is the non-thermal irreversible electroporation (NTIRE) treated area.
Figure 4
Liver tissue treated with non-thermal irreversible electroporation (NTIRE) and injected with PBS served as the control. Seven days after treatment with NTIRE and injection of PBS, immunohistochemical staining was performed on the controls using insulin and glucagon antibodies (University of Texas at Houston, USA). In the control slides, no cells were stained with insulin or glucagon antibodies, shown in (A) and (B), respectively. The higher magnification panel 4C shows normal hepatocytes with no evidence of scar tissue.
Figure 5
Liver tissue treated with non-thermal irreversible electroporation (NTIRE) and injected with 200 rat donor pancreatic islets. Seven days later, immunohistochemical staining was performed with anti-insulin and anti-glucagon antibodies (University of Texas at Houston, USA). In the pancreatic islets injected tissue, insulin-stained cell clusters are shown in (A) and (C) (full arrows). Normal hepatocytes were observed (dotted arrows) around the insulin-stained cells in the liver lobe. Glucagon-stained cells cluster are shown in (B) and (D) (full arrows). Normal hepatocytes were observed (dotted arrows) around the glucagon-stained cells in the liver lobe.