<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_raon-2021-0053_s_006">
<h3>Background</h3>
<p>The aim of this study was to determine whether <italic>in vitro</italic> maturation (IVM) of immature oocytes after controlled hormonal stimulation of the ovaries could be important in cancer patients to improve their chances of conception in the future.</p>
</sec>
<sec id="j_raon-2021-0053_s_007">
<h3>Patients and methods</h3>
<p>After ovarian stimulation in cancer patients, the number of oocytes and their quality and maturity were compared to control patients with fertility problems in the <italic>in vitro</italic> fertilization (IVF) program. In both groups of patients, immature oocytes at the developmental stage of germinal vesicle were matured <italic>in vitro</italic> and the proportion of oocytes that matured <italic>in vitro</italic> was compared between groups. In a subset of women with fertility problems, intracytoplasmic sperm injection (ICSI) was performed on IVM oocytes to assess their ability to be fertilized and develop into an embryo compared to <italic>vivo</italic> matured oocytes in the same cycles and consider the procedure in cancer patients.</p>
</sec>
<sec id="j_raon-2021-0053_s_008">
<h3>Results</h3>
<p>In patients with different cancers, the disease did not affect the number and quality of retrieved oocytes. In cancer patients, there was even a significantly lower proportion of immature oocytes than in patients with fertility problems (30.0% <italic>vs</italic>. 43.6%; P &lt; 0.05). However, in patients with cancer, fewer oocytes per patient matured <italic>in vitro</italic> than in patients with fertility problems (1.39 ± 1.04 <italic>vs</italic>. 2.48 ± 1.83; P &lt; 0.05). After ICSI, the proportions of fertilized oocytes and fertilized oocytes developing into an embryo did not differ between oocytes matured <italic>in vitro</italic> and <italic>in vivo</italic> in the same cycles.</p>
</sec>
<sec id="j_raon-2021-0053_s_009">
<h3>Conclusions</h3>
<p>Oocyte IVM is proving to be a reliable procedure for resolving immature oocytes after controlled ovarian stimulation in cancer patients.</p>
</sec>
</abstract>

Background
The aim of this study was to determine whether in vitro maturation (IVM) of immature oocytes after controlled hormonal stimulation of the ovaries could be important in cancer patients to improve their chances of conception in the future.


Patients and methods
After ovarian stimulation in cancer patients, the number of oocytes and their quality and maturity were compared to control patients with fertility problems in the in vitro fertilization (IVF) program. In both groups of patients, immature oocytes at the developmental stage of germinal vesicle were matured in vitro and the proportion of oocytes that matured in vitro was compared between groups. In a subset of women with fertility problems, intracytoplasmic sperm injection (ICSI) was performed on IVM oocytes to assess their ability to be fertilized and develop into an embryo compared to vivo matured oocytes in the same cycles and consider the procedure in cancer patients.


Results
In patients with different cancers, the disease did not affect the number and quality of retrieved oocytes. In cancer patients, there was even a significantly lower proportion of immature oocytes than in patients with fertility problems (30.0% vs. 43.6%; P < 0.05). However, in patients with cancer, fewer oocytes per patient matured in vitro than in patients with fertility problems (1.39 ± 1.04 vs. 2.48 ± 1.83; P < 0.05). After ICSI, the proportions of fertilized oocytes and fertilized oocytes developing into an embryo did not differ between oocytes matured in vitro and in vivo in the same cycles.


Conclusions
Oocyte IVM is proving to be a reliable procedure for resolving immature oocytes after controlled ovarian stimulation in cancer patients.

<italic>In vitro</italic> maturation of immature oocytes for fertility preservation in cancer patients compared to control patients with fertility problems in an <italic>in vitro</italic> fertilization program

Radiology and Oncology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 maturation of immature oocytes for fertility preservation in cancer patients compared to control patients with fertility problems in an  fertilization program

Background
The aim of this study was to determine whether in vitro maturation (IVM) of immature oocytes after controlled hormonal stimulation of the ovaries...

In vitro maturation of immature oocytes for fertility preservation in cancer patients compared to control patients with fertility problems in an in vitro fertilization program

Article Category: Research Article

Published Online: Dec 22, 2021

Page range: 119 - 128

Received: Aug 05, 2021

Accepted: Nov 25, 2021

DOI: https://doi.org/10.2478/raon-2021-0053

© 2022 Irma Virant-Klun, Jure Bedenk, Nina Jancar, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.