<abstract xmlns="http://www.w3.org/1999/xhtml">

Honey can easily be adulterated with various cheaper sweeteners for higher commercial profits. Commonly used adulterants include high fructose maize syrup and refined cane sugar, which origin is C4 plants. Isotope ratio mass spectrometry (IRMS) can be used to determine the adulteration of honey with C4 plants. A significant δ13C value difference between honey and its protein provide valuable information of honey authenticity. The IRMS analysis of honey and its proteins and ultra high performance liquid chromatography – evoparative light scattering detection analysis for adulterated honey were performed to assess honey authenticity and origin. Principal component analysis of the obtained IRMS data was performed in order to determine discrimination between groups and for visualisation of observations. The results showed that isotope ratio values in combination with principal component analysis could be useful for determination of adulterant and discrimination of honey of different geographical origins. The chromatography results show ascending increase of the deliberately added sucrose, but do not fully recover the expected value.
</abstract>

Honey can easily be adulterated with various cheaper sweeteners for higher commercial profits. Commonly used adulterants include high fructose maize syrup and refined cane sugar, which origin is C4 plants. Isotope ratio mass spectrometry (IRMS) can be used to determine the adulteration of honey with C4 plants. A significant δ13C value difference between honey and its protein provide valuable information of honey authenticity. The IRMS analysis of honey and its proteins and ultra high performance liquid chromatography – evoparative light scattering detection analysis for adulterated honey were performed to assess honey authenticity and origin. Principal component analysis of the obtained IRMS data was performed in order to determine discrimination between groups and for visualisation of observations. The results showed that isotope ratio values in combination with principal component analysis could be useful for determination of adulterant and discrimination of honey of different geographical origins. The chromatography results show ascending increase of the deliberately added sucrose, but do not fully recover the expected value.

Determination of Sucrose Additives and Geographical Origin Markers in Honey Using Isotope Ratio Mass Spectrometry and Ultra High Performance Liquid Chromatography – Evoparative Light Scattering Detection

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Honey can easily be adulterated with various cheaper sweeteners for higher commercial profits. Commonly used adulterants include high fructose maize syrup and...

Determination of Sucrose Additives and Geographical Origin Markers in Honey Using Isotope Ratio Mass Spectrometry and Ultra High Performance Liquid Chromatography – Evoparative Light Scattering Detection

Published Online: Mar 03, 2022

Page range: 152 - 156

Received: Mar 22, 2021

Accepted: Jan 09, 2022

DOI: https://doi.org/10.2478/prolas-2022-0023

Keywords
honey, IRMS, UHPLC, C plants, principal component analysis

© 2022 Krišs Dāvids Labsvārds et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Determination of Sucrose Additives and Geographical Origin Markers in Honey Using Isotope Ratio Mass Spectrometry and Ultra High Performance Liquid Chromatography – Evoparative Light Scattering Detection

Published Online: Mar 03, 2022

Page range: 152 - 156

Received: Mar 22, 2021

Accepted: Jan 09, 2022

DOI: https://doi.org/10.2478/prolas-2022-0023

Keywordshoney, IRMS, UHPLC, C plants, principal component analysis

© 2022 Krišs Dāvids Labsvārds et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
honey, IRMS, UHPLC, C plants, principal component analysis