The paper presents a continuation of studies on the influence of increased ambient pressure on the eye-muscle reflex. Using the same methods, the research was carried out in real conditions, i.e. during dives in water. Dives were performed in classical equipment with air as a breathing mix at depths of 10, 20, 30, 40, 50 and 60 m. It was found that statistically significant differences in the time of eye-muscle reflexes occur during the transition from 0 to 10, from 40 to 50 and 50 to 60 meters of overpressure. In the conclusions, it was found that the prolongation of reflex time is much greater than in hyperbaric chamber studies. As in previous studies, toxic effects of components of the breathing mixture, especially nitrogen, were considered the main cause.