<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Inonotus obliquus</italic> is a parasite on the birch and other trees and is also a well-known medicinal mushroom. Its sterile conk is highly sought for its bioactive compounds such as phenols, polysaccharides, triterpenoids, and steroids. It was traditionally used to treat various gastrointestinal diseases, viral and parasitic infections, to counteract the progression of cancers, and to stimulate the immune system. We used acute gamma irradiation, followed by short-term submerged cultivation, as an oxidative stress inducer to enhance the synthesis of mycelial metabolites. The 300 Gy and 400 Gy doses showed the best results across the whole experimental design. Each assayed criterion had a different corresponding optimal stimulation dose. In one experiment, sublethal doses of irradiation triggered the dry weight of the cultured mycelium to increase by 19.764%. The free radical scavenging potential of the mycelium extracts increased by 79.83%. The total phenolic content of mycelium extracts and culture broth increased by 55.7% and 62.987%, respectively. The total flavonoid and sinapinic acid content of the broth increased by 934.678% and 590.395%, respectively. As such, gamma irradiation pre-treatment of the mycelial inoculum proved an interesting, economically and environmentally effective tool for stimulating secondary metabolite synthesis in submerged mycelium cultures.</p>
</abstract>

Inonotus obliquus is a parasite on the birch and other trees and is also a well-known medicinal mushroom. Its sterile conk is highly sought for its bioactive compounds such as phenols, polysaccharides, triterpenoids, and steroids. It was traditionally used to treat various gastrointestinal diseases, viral and parasitic infections, to counteract the progression of cancers, and to stimulate the immune system. We used acute gamma irradiation, followed by short-term submerged cultivation, as an oxidative stress inducer to enhance the synthesis of mycelial metabolites. The 300 Gy and 400 Gy doses showed the best results across the whole experimental design. Each assayed criterion had a different corresponding optimal stimulation dose. In one experiment, sublethal doses of irradiation triggered the dry weight of the cultured mycelium to increase by 19.764%. The free radical scavenging potential of the mycelium extracts increased by 79.83%. The total phenolic content of mycelium extracts and culture broth increased by 55.7% and 62.987%, respectively. The total flavonoid and sinapinic acid content of the broth increased by 934.678% and 590.395%, respectively. As such, gamma irradiation pre-treatment of the mycelial inoculum proved an interesting, economically and environmentally effective tool for stimulating secondary metabolite synthesis in submerged mycelium cultures.

The use of gamma irradiation to stimulate bioactive compound synthesis in <italic>Inonotus obliquus</italic> submerged cultures

Romania and University of Agricultural Sciences and Veterinary Medicine of Bucharest

University of Agricultural Sciences and Veterinary Medicine of Bucharest

Nukleonika

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The use of gamma irradiation to stimulate bioactive compound synthesis in  submerged cultures

Inonotus obliquus is a parasite on the birch and other trees and is also a well-known medicinal mushroom. Its sterile conk is highly sought for its bioactive...

The use of gamma irradiation to stimulate bioactive compound synthesis in Inonotus obliquus submerged cultures

Article Category: Original Paper

Published Online: Sep 17, 2021

Page range: 83 - 90

Received: Feb 02, 2021

Accepted: Jun 07, 2021

DOI: https://doi.org/10.2478/nuka-2021-0012

Keywords
Gamma irradiation, Chaga, Medicinal fungi, Metabolite synthesis, Bioactive compounds

© 2021 Alexandru Petre et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The use of gamma irradiation to stimulate bioactive compound synthesis in Inonotus obliquus submerged cultures

Article Category: Original Paper

Published Online: Sep 17, 2021

Page range: 83 - 90

Received: Feb 02, 2021

Accepted: Jun 07, 2021

DOI: https://doi.org/10.2478/nuka-2021-0012

KeywordsGamma irradiation, Chaga, Medicinal fungi, Metabolite synthesis, Bioactive compounds

© 2021 Alexandru Petre et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Gamma irradiation, Chaga, Medicinal fungi, Metabolite synthesis, Bioactive compounds