1. bookVolume 15 (2019): Issue 1 (March 2019)
Journal Details
First Published
12 Apr 2013
Publication timeframe
4 times per year
access type Open Access

Determine the Required Log Reductions of Human Intestinal Helminth Eggs by Waste Stabilization Pond: A Simulation for Wastewater Recycling in Agriculture

Published Online: 20 Jun 2019
Volume & Issue: Volume 15 (2019) - Issue 1 (March 2019)
Page range: 11 - 25
Journal Details
First Published
12 Apr 2013
Publication timeframe
4 times per year

This paper investigates the determined the required log reductions for human intestinal helminth eggs by waste stabilization ponds as simulation as assessing of mitigating health risk to satisfy practice WHO, 2006 guidelines for the safe use of wastewater in agriculture (≤ 0.1 helminth egg/L) to protect the health of children under 15 years was the development of MATLAB, a computer program based waste stabilization ponds design based on parameter uncertainty and 10,000-trial Monte Carlo simulations were developed for a series of anaerobic, facultative and maturation ponds based on 95%-ile of effluent (≤ 0.1 helminth egg/L) which the result in a health-based target. Whereas the influent of the helminth eggs (Nematode) was (932.500 eggs/L). While the treatment provided (100 % reduction/removal) for the overall treatment process with total hydraulic retention time in climatic conditions of Libya it took 36.207 days in the anaerobic pond, facultative pond, first maturation pond and one of the subsequent maturation pond.


[1] Data and statistics. (2005). World Bank, 2000. (http://www.worldbank.org/data, accessed 7 April.Search in Google Scholar

[2] Food and Agriculture Organization of the United Nations (FAO). (2012). Coping with water scarcity: An action framework for agriculture and food security. FAO Water Reports, No.38. Rome.Search in Google Scholar

[3] Raschid-Sally, Liqa, and Priyantha Jayakody. (2008). Drivers and characteristics of wastewater agriculture in developing countries: Results from a global assessment. Colombo, Sri Lanka: International Water Management Institute. 35p. (IWMI Research Report 127).Search in Google Scholar

[4] Food and Agriculture Organization of the United Nations (FAO). (2013). Wastewater reuse and agriculture: benefits for all Report on water issues 35, FAO, Rome.Search in Google Scholar

[5] Nazari, R. Eslamian, S. Khanbilvardi, R. (2012). Water reuse and sustainability. In: Voudouris K (eds) Ecological water quality-Water treatment and reuse. InTech, Rijeka, Croatia and Shanghai, China 241-254.Search in Google Scholar

[6] Gatto D’Andrea ML, Salas Barboza AGJ, Garcés V, Rodriguez-Alvarez MS, Iribarnegaray MA, et al. (2015). The Use of (Treated) Domestic Wastewater for Irrigation: Current Situation and Future Challenges. Int J Water and Wastewater Treatment 1(2): doi 2381-5299Search in Google Scholar

[7] Carr, G. Potter, RB. Nortcliff, S. (2011). Water reuse for irrigation in Jordan: perceptions of water quality among farmers. Agric Water Manag 98: 847–85410.1016/j.agwat.2010.12.011Search in Google Scholar

[8] Hanjra, MA. Blackwell, J. Carr, G. Zhang, FH. Jackson, TM. (2012). Wastewater irrigation and environmental health: implications for water governance and public policy. Int J Hyg Environ Health 215:255–26910.1016/j.ijheh.2011.10.003Search in Google Scholar

[9] Ahmed, W. Sidhu, JPS. Smith, K. Beale, DJ. Gyawali, P. Toze, S. (2016). Distributions of fecal markers in wastewater from different climatic zones for human fecal pollution tracking in Australian surface waters. Appl Environ Microbiol 82:1316–132310.1128/AEM.03765-15Search in Google Scholar

[10] Petterson, SR. Stenström, TA. Ottoson, J. (2016). A theoretical approach to using faecal indicator data to model norovirus concentration in surface water for QMRA: Glomma River, Norway. Water Res 91:31–3710.1016/j.watres.2015.12.037Search in Google Scholar

[11] Amoah, ID. Reddy, P. Seidu, R. Stenström, TA. (2018). Removal of helminth eggs by centralized and decentralized wastewater treatment plants in South Africa and Lesotho: health implications for direct and indirect exposure to the effluents. Environ. Sci. Pollut. Res. 25:12883–1289510.1007/s11356-018-1503-7Search in Google Scholar

[12] Kamizoulis, G. (2008). Setting health based targets for water reuse (in agriculture). Science Direct 218 154–163.10.1016/j.desal.2006.08.026Search in Google Scholar

[13] Yates, M.V. Gerba, C.P. (1998). Microbial considerations in wastewater reclamation and reuse. In: T. Asano, ed., Wastewater Reclamation and Reuse. Technomic Publishing Company, Lancaster, Pennsylvania, Chap. 10, pp. 1–56.Search in Google Scholar

[14] Feachem, R.G. Bradley, D.J. Garelick, H. Mara, D.D. (1983). Sanitation and disease: health aspects of excreta and wastewater management. World Bank Studies in Water Supply and Sanitation 32, John Wiley & Sons, ChichesterSearch in Google Scholar

[15] Mara, D.D. Silva, S.A. (1986). Removal of intestinal nematode eggs in tropical waste stabilization ponds. J. Trop. Medicine Hygiene, 89(2) 71–74.Search in Google Scholar

[16] Oragui, J.I. Curtis, T.P. Silva, S.A. Mara, D.D. (1987). Removal of excreted bacteria and viruses in deep waste stabilization ponds in northeast Brazil. Wat. Sci. Technol., 19 (Rio) 569–573.10.2166/wst.1987.0236Search in Google Scholar

[17] Jimenez-Cisneros, B.E. Maya-Rendon, C. (2007). Helminths and Sanitation. Communicating Current Research and Educational Topics and Trends in Applied MicrobiologySearch in Google Scholar

[18] Atlas of Medical Parasitology, Intestinal parasites helminths, Tropical and Parasitology Service (2000). Amadeo di Savoia Hospital. The editor Editor Pietro Caramello MD.Search in Google Scholar

[19] World Health Organisation. Health Guidelines for the use of wastewater in agriculture and aquaculture. (1989). Wld. Hlth. Org. Techn. Rep. Ser. No. 778.Search in Google Scholar

[20] Horan, N. J. (1990). Biological Wastewater Treatment Systems “Theory and Operation”. John Willey & Sons Ltd. New York.Search in Google Scholar

[21] Al-Salem, S. S. Lumbers, J. P. (1987). An initial evaluation of Al-Samra waste stabilization ponds (Jordan). Water Science and Technology, 19 (12): 33-37.Search in Google Scholar

[22] Bartone, C. R. Arlosoroff, S. (1987). Irrigation reuse of pond effluents in developing countries. Wat. Sci. Tech., 19 (12): 289-297.Search in Google Scholar

[23] Schwartzbrod, J. Bouhoum, K. Baleux, B. (1987). Effects of lagoon treatment on helminth eggs. Water Science and Technology, 19: 369-371.Search in Google Scholar

[24] Mara, D. D. Pearson, H. W. Alabaster, G. Mills. S. (1990). An Evaluation Waste Stabilization Ponds in Kenya. ODA Research Scheme R4442A, Final Report.Search in Google Scholar

[25] Saqqar, M. Pescod, M. B. (1991). Microbiological performance of multi-stage stabilization ponds from effluent use in agriculture. Water Science and Technology, 23 (7-9): 1517-1524.Search in Google Scholar

[26] Saqqar, M. Pescod, M. B. (1992). Modeling nematode egg elimination in wastewater stabilization ponds. Water Science and Technology, 26 (7-8): 1659-1665.Search in Google Scholar

[27] Ayres, R. M. Lee, D. L. Mara, D. D. Silva, S. A. (1993). The accumulation, distribution and viability of human parasitic nematode eggs in the sludge of primary facultative waste stabilisation pond. Transactions of the Royal Society of Tropical Medicine and Hygiene, 87 (2): 256-258.Search in Google Scholar

[28] World Health Organization. Integrated Guide to Sanitary Parasitology. (2004). Regional office for the Eastern Mediterranean, regional centre for environmental health activities, Amman – JordanSearch in Google Scholar

[29] WHO, Guidelines for the Safe Use of Wastewater, Excreta and Greywater, (2006). Vol. II, Wastewater Use in Agriculture, World Health Organization, United Nations Environmental Program, United Nations Food and Agriculture Organization, Geneva, SwitzerlandSearch in Google Scholar

[30] Libyan National Meteorological Center (LNMC) Climate DepartmentSearch in Google Scholar

[31] APHA. Standard methods for the examination of water and wastewater, (1998). (20th edition, American Public Health Association, Washington DC, 1325).Search in Google Scholar

[32] Ayres, R.M. Mara, D.D. (1996). Analysis of Wastewater for Use in Agriculture: A Laboratory Manual of Parasitoligical and Bacteriological Techniques. Geneva, Switzerland: World Health OrganizationSearch in Google Scholar

[33] Banda, C.G. (2003). Modern Design of Waste Stabilization Ponds in Warm Climates: Comparison with Traditional Design Methods. MSc. thesis, University of Leeds, Leeds, UKSearch in Google Scholar

[34] Banda, C.G. Sleigh, P.A. Mara, D.D. (2005). Escherichia coli removal in waste stabilization ponds: a comparison of modern and classical designs. Wat. Scie. & Tech. Vol 51 No 12 pp 75–8110.2166/wst.2005.0431Search in Google Scholar

[35] Von Sperling, M. (1996). Design of facultative pond based on uncertainty analysis. Wat. Scie. & Tech., 33 (7), 41-4710.2166/wst.1996.0120Search in Google Scholar

[36] Vose, D. (1996). Quantitative Risk Analysis: A Guide to Monte Carlo Simulation Modelling. John Wiley and Sons Ltd, Chichester, England.Search in Google Scholar

[37] Mara, D.D. Pearson, H.W. (1986). Artificial freshwater environments: waste stabilization ponds. In Biotechnology, vol. 8 (ed. W. Schoenborn), pp. 177-206. VCHSearch in Google Scholar

[38] Mara, D.D. Pearson, H.W. Arridge, J.I. Arridge, H. Silva, S.A.. (1997). Development of a New Approach to Waste Stabilization Pond Design. TPHE Research MonographSearch in Google Scholar

[39] Marais, G.V.R. (1974). Faecal bacterial kinetics in waste stabilization ponds. Journal of the Env. Eng. Divis., ASCE, 100 (EE1), 119-139Search in Google Scholar

[40] Ayres, R.M. Alabaster, G.P. Mara, D.D. Lee, D.L. (1992). A design equation for human intestinal nematode egg removal in waste stabilization ponds. Water Research 26 (6), 863–865.10.1016/0043-1354(92)90019-ZSearch in Google Scholar

[41] Ayres, R. M. Mara, D. D. Lee, D. L. Thitai, W. N. (1993). Monitoring full scale waste stabilization pond in Kenya for Nematode egg removal. Environmental Technology, Vol. 14. pp 295-300.10.1080/09593339309385293Search in Google Scholar

[42] Mara, D. D. (1987). Waste stabilization ponds; Problems and controversies, Wat. Qual. Int., 1, 20-22Search in Google Scholar

[43] Mara, D.D. (2004). Domestic wastewater treatment in developing countries, E./J. & J., London, UK.Search in Google Scholar

[44] Mara, D. D. Alabaster, G. P. Pearson, H. W. Mills, S. W. (1992). Waste stabilization ponds: A design manual for Eastern Africa, Lag. Techn. Intern., Leeds, England, 591 – 594.Search in Google Scholar

[45] Verbyla, M. von Sperling, M. Maiga, Y. (2017). Waste Stabilization Ponds. In: J.B. Rose and B. Jiménez-Cisneros, (eds) Global Water Pathogens Project.Search in Google Scholar

[46] Templeton, M. R. Andrews, R. C. Hofmann, R. (2008). Particle-Associated Viruses in Water: Impacts on Disinfection Processes. Critical Reviews in Environmental Science and Technology, 38, 137-16410.1080/10643380601174764Search in Google Scholar

[47] Reinoso, R. Blanco, S. Torres-Villamizar L. A. Becares, E. (2011). Mechanisms for parasites removal in a waste stabilisation pond. Microbial Ecology, 61, 684-69210.1007/s00248-010-9791-621207019Search in Google Scholar

[48] Stratton, H. Lemckert, C. Roiko, A. Zhang, Wilson, H. S. Gibb, K. van der Akker, B. Macdonald, J. Melvin, S. Sheludchenko, M. Li, M., Xie, Padovan, J. A. Lehmann, R. (2015). Validation of maturation ponds in order to enhance safe and economical water recycling, Australian Water Recycling Centre of Excellence, Brisbane Australia.Search in Google Scholar

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