<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Acute <italic>B. canis</italic> infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with <italic>B. canis</italic>. Metabolic and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital staining. Both young and adult dogs with acute <italic>B. canis</italic> infection showed non-regenerative anemia without difference. Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte count was observed (p&lt;0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs, although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease in ApoA I in both groups 14 days after treatment (p&lt;0.01) confirmed that this lipoprotein acts as a positive acute-phase protein in acute <italic>B. canis</italic> infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.</p>
</abstract>

Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing anemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of the erythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parameters focusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days after treatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolic and inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified time points. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I was assessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravital staining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference. Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocyte count was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs, although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decrease in ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phase protein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.

Reticulocyte Response in Paired Blood Samples of <italic>Babesia canis</italic> Infected Young and Adult Dogs

Laboratory for Radioisotopes, Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution 4.0 International License.

Reticulocyte Response in Paired Blood Samples of  Infected Young and Adult Dogs

Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causing anemia through immune-mediated...

Reticulocyte Response in Paired Blood Samples of Babesia canis Infected Young and Adult Dogs

Published Online: Feb 07, 2024

Page range: 13 - 21

Received: Nov 29, 2023

Accepted: Jan 15, 2024

DOI: https://doi.org/10.2478/macvetrev-2024-0011

Keywords
dog, reticulocyte count, paired blood samples, ApoA I

© 2024 Zorana Milanović et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Reticulocyte Response in Paired Blood Samples of Babesia canis Infected Young and Adult Dogs

Published Online: Feb 07, 2024

Page range: 13 - 21

Received: Nov 29, 2023

Accepted: Jan 15, 2024

DOI: https://doi.org/10.2478/macvetrev-2024-0011

Keywordsdog, reticulocyte count, paired blood samples, ApoA I

© 2024 Zorana Milanović et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
dog, reticulocyte count, paired blood samples, ApoA I