<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Semen cooling and cryopreservation have revolutionized the field of reproductive biotechnologies. However, challenges persist in maintaining sperm quality and viability during these processes. The unsatisfactory results and the main problems are associated with low quality, viability, morphological, structural, and DNA integrity, changes in plasma membrane, ability to interact with female tract and decrease in sperm fertilization potential. The objective of this study was to determine the effect of seminal plasma proteins with different molecular weights on the kinetic parameters of dog spermatozoa during cold storage at 4 °C. The proteins were isolated from the dogs’ seminal plasma ranging between 6-200 kDa, separated in four fractions. The ejaculates (n=15) were collected from 6 heathy dogs which were pooled. Spermatozoa were extended with Tris medium alone (control) or with addition of one of the isolated protein fractions, and were then incubated at 4 °C for 2 hours. Sperm incubated with seminal plasma proteins ranging between 10-15 kDa had significantly higher total motility (37.01±2.98%), sperm progressiveness (15.97±1.91%), curvilinear line velocity (37.46±3.75 μm/s), linearity (26.18±1.00%), and straightness (45.94±2.03%) compared to the other groups incubated with higher molecular weight proteins and the control group. The findings of this study indicated that the use of certain proteins in seminal plasma can be beneficial for reducing the detrimental effect of cooling at 4 °C and preserving the viability of dog spermatozoa. It seemed that the presence of 10-15 kDa proteins from canine seminal plasma rendered the spermatozoa less amenable to the negative influence during cooling.</p>
</abstract>

Semen cooling and cryopreservation have revolutionized the field of reproductive biotechnologies. However, challenges persist in maintaining sperm quality and viability during these processes. The unsatisfactory results and the main problems are associated with low quality, viability, morphological, structural, and DNA integrity, changes in plasma membrane, ability to interact with female tract and decrease in sperm fertilization potential. The objective of this study was to determine the effect of seminal plasma proteins with different molecular weights on the kinetic parameters of dog spermatozoa during cold storage at 4 °C. The proteins were isolated from the dogs’ seminal plasma ranging between 6-200 kDa, separated in four fractions. The ejaculates (n=15) were collected from 6 heathy dogs which were pooled. Spermatozoa were extended with Tris medium alone (control) or with addition of one of the isolated protein fractions, and were then incubated at 4 °C for 2 hours. Sperm incubated with seminal plasma proteins ranging between 10-15 kDa had significantly higher total motility (37.01±2.98%), sperm progressiveness (15.97±1.91%), curvilinear line velocity (37.46±3.75 μm/s), linearity (26.18±1.00%), and straightness (45.94±2.03%) compared to the other groups incubated with higher molecular weight proteins and the control group. The findings of this study indicated that the use of certain proteins in seminal plasma can be beneficial for reducing the detrimental effect of cooling at 4 °C and preserving the viability of dog spermatozoa. It seemed that the presence of 10-15 kDa proteins from canine seminal plasma rendered the spermatozoa less amenable to the negative influence during cooling.

Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics

Department of Reproduction Biology and Biotechnology of the Gametes

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution 4.0 International License.

{"article-title":"Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics"}

Semen cooling and cryopreservation have revolutionized the field of reproductive biotechnologies. However, challenges persist in maintaining sperm quality and...

Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics

Published Online: Sep 28, 2023

Page range: 177 - 183

Received: Nov 28, 2022

Accepted: Sep 13, 2023

DOI: https://doi.org/10.2478/macvetrev-2023-0027

Keywords
spermatozoa, seminal plasma proteins, cooling, kinetics, dog

© 2023 Tsvetan Stefanov Tsvetkov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of Seminal Plasma Protein Fractions on Cooled Dog Semen Kinetics

Published Online: Sep 28, 2023

Page range: 177 - 183

Received: Nov 28, 2022

Accepted: Sep 13, 2023

DOI: https://doi.org/10.2478/macvetrev-2023-0027

Keywordsspermatozoa, seminal plasma proteins, cooling, kinetics, dog

© 2023 Tsvetan Stefanov Tsvetkov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
spermatozoa, seminal plasma proteins, cooling, kinetics, dog