<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>β-lactamases are a diverse class of enzymes produced by bacteria that present a major cause for resistance to β-lactams. In this study we analysed 159 fecal samples from dairy cows, for the presence of presumptive ESBL, AmpC, and carbapenemase-producing <italic>E. coli</italic>. Phylotyping was done using Clermont phylo-typing method, targeting arpA, ChuA, and YjaA genes, along with the DNA fragment TspE4.C2. Convetional PCR method was used to confirm the presence of <italic>bla</italic> genes among 39 phenotypically confirmed ESBL producing <italic>E. coli.</italic> The results showed presence of CTX-M, SHV, TEM and OXA1 <italic>bla</italic> genes in 28 (71.79%), 1 (2.56%), 29 (74.35%), 2 (5.12%) of isolates, respectively Twenty (51.28%) isolates showed presence of both <italic>bla</italic>CTX-M and TEM genes. The strain that carried the <italic>bla</italic>SHV gene was found to carry <italic>bla</italic>TEM gene as well, while one of the strains that carried <italic>bla</italic>OXA1 gene was also carrying <italic>bla</italic>CTX-M and TEM gene. The ration between isolates and phylo-groups was as follows: 9 (23.07%) strains were assigned to phyllo-group D; 14 (35.89%) to phyllo-group B; 16 (41.02%) to phyllo-group A. Out of the 39 strains where <italic>bla</italic> genes were identified, 29 (74.35%) were categorized as multi drug resistant.</p>
</abstract>

β-lactamases are a diverse class of enzymes produced by bacteria that present a major cause for resistance to β-lactams. In this study we analysed 159 fecal samples from dairy cows, for the presence of presumptive ESBL, AmpC, and carbapenemase-producing E. coli. Phylotyping was done using Clermont phylo-typing method, targeting arpA, ChuA, and YjaA genes, along with the DNA fragment TspE4.C2. Convetional PCR method was used to confirm the presence of bla genes among 39 phenotypically confirmed ESBL producing E. coli. The results showed presence of CTX-M, SHV, TEM and OXA1 bla genes in 28 (71.79%), 1 (2.56%), 29 (74.35%), 2 (5.12%) of isolates, respectively Twenty (51.28%) isolates showed presence of both blaCTX-M and TEM genes. The strain that carried the blaSHV gene was found to carry blaTEM gene as well, while one of the strains that carried blaOXA1 gene was also carrying blaCTX-M and TEM gene. The ration between isolates and phylo-groups was as follows: 9 (23.07%) strains were assigned to phyllo-group D; 14 (35.89%) to phyllo-group B; 16 (41.02%) to phyllo-group A. Out of the 39 strains where bla genes were identified, 29 (74.35%) were categorized as multi drug resistant.

β-Lactamase Genes (<italic>bla</italic>CTX-M, <italic>bla</italic>SHV, <italic>bla</italic>TEM, <italic>bla</italic>OXA1 and <italic>bla</italic>OXA2) and Phylogenetic Groups in ESBL Producing Commensal <italic>Escherichia coli</italic> Isolated from Faecal Samples from Dairy Farm in the Municipality of Debar

Ss. Cyril and Methodius University in Skopje

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution 4.0 International License.

β-Lactamase Genes (CTX-M, SHV, TEM, OXA1 and OXA2) and Phylogenetic Groups in ESBL Producing Commensal  Isolated from Faecal Samples from Dairy Farm in the Municipality of Debar

β-lactamases are a diverse class of enzymes produced by bacteria that present a major cause for resistance to β-lactams. In this study we analysed 159 fecal...

β-Lactamase Genes (blaCTX-M, blaSHV, blaTEM, blaOXA1 and blaOXA2) and Phylogenetic Groups in ESBL Producing Commensal Escherichia coli Isolated from Faecal Samples from Dairy Farm in the Municipality of Debar

Published Online: Mar 08, 2023

Page range: 89 - 97

Received: Nov 18, 2022

Accepted: Jan 30, 2023

DOI: https://doi.org/10.2478/macvetrev-2023-0017

Keywords
ESBL, genes, phylogenetic groups, dairy cow

© 2023 Maksud Kerluku et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

β-Lactamase Genes (blaCTX-M, blaSHV, blaTEM, blaOXA1 and blaOXA2) and Phylogenetic Groups in ESBL Producing Commensal Escherichia coli Isolated from Faecal Samples from Dairy Farm in the Municipality of Debar

Published Online: Mar 08, 2023

Page range: 89 - 97

Received: Nov 18, 2022

Accepted: Jan 30, 2023

DOI: https://doi.org/10.2478/macvetrev-2023-0017

KeywordsESBL, genes, phylogenetic groups, dairy cow

© 2023 Maksud Kerluku et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
ESBL, genes, phylogenetic groups, dairy cow