<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Transmissible spongiform encephalopathies (TSEs) are a group of neurodegenerative diseases with a chronic and fatal course, which are caused by a misfolded form of the cellular prion protein that is encoded by the host. The purpose of this work was to evaluate the resistance and genetic susceptibility to prion diseases in Pelibuey sheep from Mexico. The sequences of 99 Pelibuey sheep from the central and southwestern regions of Mexico were analyzed to determine the polymorphisms related to resistance and genetic susceptibility to scrapie, the Hardy-Weinberg equilibrium test and the D-Tajima test was used to identify the effect of evolutionary forces on the <italic>PRNP</italic> gene. Twelve non-synonymous polymorphisms Q101R, M112T, A116P, G127A, A136V, M137T, L141F, H143R, R154H, Q171R, Q171H, N176K were detected, in addition two synonymous substitutions 231R (agg/cgg) and 237L (ctc/ctg) were found. As a result of the sequence analysis, the ARR allele was not under the Hardy-Weinberg equilibrium, indicating that there is an evolutionary force at work, and the D-Tajima suggest the existence of purifying genetic selection. In conclusion, Pelibuey sheep exhibit genotypes for the <italic>PRNP</italic> gene that make them resistant to developing classic scrapie; at least 98% of the population is a carrier of an allele related to scrapie resistance; while for atypical scrapie there is a very high probability that an outbreak occurred in the herds, since the ARQ allele in combination with the L substitution at codon 141 confers susceptibility to carrier sheep.</p>
</abstract>

Transmissible spongiform encephalopathies (TSEs) are a group of neurodegenerative diseases with a chronic and fatal course, which are caused by a misfolded form of the cellular prion protein that is encoded by the host. The purpose of this work was to evaluate the resistance and genetic susceptibility to prion diseases in Pelibuey sheep from Mexico. The sequences of 99 Pelibuey sheep from the central and southwestern regions of Mexico were analyzed to determine the polymorphisms related to resistance and genetic susceptibility to scrapie, the Hardy-Weinberg equilibrium test and the D-Tajima test was used to identify the effect of evolutionary forces on the PRNP gene. Twelve non-synonymous polymorphisms Q101R, M112T, A116P, G127A, A136V, M137T, L141F, H143R, R154H, Q171R, Q171H, N176K were detected, in addition two synonymous substitutions 231R (agg/cgg) and 237L (ctc/ctg) were found. As a result of the sequence analysis, the ARR allele was not under the Hardy-Weinberg equilibrium, indicating that there is an evolutionary force at work, and the D-Tajima suggest the existence of purifying genetic selection. In conclusion, Pelibuey sheep exhibit genotypes for the PRNP gene that make them resistant to developing classic scrapie; at least 98% of the population is a carrier of an allele related to scrapie resistance; while for atypical scrapie there is a very high probability that an outbreak occurred in the herds, since the ARQ allele in combination with the L substitution at codon 141 confers susceptibility to carrier sheep.

Evaluation of Resistance and Susceptibility to Prion Diseases in Pelibuey Sheep from Mexico

Benemérita Universidad Autónoma de Puebla

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution 4.0 International License.

Transmissible spongiform encephalopathies (TSEs) are a group of neurodegenerative diseases with a chronic and fatal course, which are caused by a misfolded...

Evaluation of Resistance and Susceptibility to Prion Diseases in Pelibuey Sheep from Mexico

Published Online: Dec 31, 2022

Page range: 17 - 26

Received: Apr 26, 2022

Accepted: Dec 01, 2022

DOI: https://doi.org/10.2478/macvetrev-2022-0030

Keywords
gene, polymorphisms, genetic susceptibility, genetic selection, Pelibuey

© 2023 Fabiola Rodríguez-Andrade et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Evaluation of Resistance and Susceptibility to Prion Diseases in Pelibuey Sheep from Mexico

Published Online: Dec 31, 2022

Page range: 17 - 26

Received: Apr 26, 2022

Accepted: Dec 01, 2022

DOI: https://doi.org/10.2478/macvetrev-2022-0030

Keywordsgene, polymorphisms, genetic susceptibility, genetic selection, Pelibuey

© 2023 Fabiola Rodríguez-Andrade et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
gene, polymorphisms, genetic susceptibility, genetic selection, Pelibuey