<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The myocardium of diabetic subjects displays reduced HSP70 protein level and weak myocardial protection. However, the heart possesses an ability to produce heat shock proteins (HSPs) after exposure to sublethal heat stress. Acetylsalicylicacid (ASA) has the property of pharmacological induction of HSPs. We evaluated the common effects of single dose ASA-pretreatment, prior to heat preconditioning (HP), over carbohydrate metabolism-related enzymes and substrates in the heart of diabetic rats. Streptozotocin-diabetes caused significant decrease of HSP70 protein level, stimulation of the gluconeogenic processes and inhibition of glycolytic processes in the heart. HP-diabetic hearts have significantly higher HSP70 protein level, lower glycogen, glucose-6-phosphate content, glycogen phosphorylase and hexokinase activity, and higher glucose levels and PFK activity. ASA-pretreatment of HP-diabetic animals caused additional increase of HSP70, additional decrease of glycogen, glucose-6-phosphate, glycogen phosphorylase and hexokinase, and additional increase of glucose and PFK in the heart. In conclusion, HP is physiological inducer of HSP70 level in heart and tends to reverse carbohydrate - related disturbances in diabetic rats. ASA, given prior to HP, is a potent HSP70 co-inducer and causes additional increase of HSP70 protein level in heart. ASA, given in a combination to HP, have shown more evident protective effects against subsequent intense of stress.</p>
</abstract>

The myocardium of diabetic subjects displays reduced HSP70 protein level and weak myocardial protection. However, the heart possesses an ability to produce heat shock proteins (HSPs) after exposure to sublethal heat stress. Acetylsalicylicacid (ASA) has the property of pharmacological induction of HSPs. We evaluated the common effects of single dose ASA-pretreatment, prior to heat preconditioning (HP), over carbohydrate metabolism-related enzymes and substrates in the heart of diabetic rats. Streptozotocin-diabetes caused significant decrease of HSP70 protein level, stimulation of the gluconeogenic processes and inhibition of glycolytic processes in the heart. HP-diabetic hearts have significantly higher HSP70 protein level, lower glycogen, glucose-6-phosphate content, glycogen phosphorylase and hexokinase activity, and higher glucose levels and PFK activity. ASA-pretreatment of HP-diabetic animals caused additional increase of HSP70, additional decrease of glycogen, glucose-6-phosphate, glycogen phosphorylase and hexokinase, and additional increase of glucose and PFK in the heart. In conclusion, HP is physiological inducer of HSP70 level in heart and tends to reverse carbohydrate - related disturbances in diabetic rats. ASA, given prior to HP, is a potent HSP70 co-inducer and causes additional increase of HSP70 protein level in heart. ASA, given in a combination to HP, have shown more evident protective effects against subsequent intense of stress.

Carbohydrate Metabolism in Diabetic Rat’s Heart – The Effects of Acetylsalicylic Acid and Heat Preconditioning as HSP70 Inducers

Department of Experimental Physiology and Biochemistry

Faculty of Natural Sciences and Mathematics

Ss. Cyril and Methodius University in Skopje

Department for Pathophysiology, Faculty of Veterinary Medicine

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The myocardium of diabetic subjects displays reduced HSP70 protein level and weak myocardial protection. However, the heart possesses an ability to produce...

Carbohydrate Metabolism in Diabetic Rat’s Heart – The Effects of Acetylsalicylic Acid and Heat Preconditioning as HSP70 Inducers

Published Online: Nov 23, 2022

Page range: 137 - 148

Received: Feb 02, 2022

Accepted: Jun 06, 2022

DOI: https://doi.org/10.2478/macvetrev-2022-0021

Keywords
heat preconditioning, acetylsalicylic acid, streptozotocin-induced diabetes, heart, carbohydrate-related enzymes and substrates

© 2022 Mirsada Dervisevik et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Carbohydrate Metabolism in Diabetic Rat’s Heart – The Effects of Acetylsalicylic Acid and Heat Preconditioning as HSP70 Inducers

Published Online: Nov 23, 2022

Page range: 137 - 148

Received: Feb 02, 2022

Accepted: Jun 06, 2022

DOI: https://doi.org/10.2478/macvetrev-2022-0021

Keywordsheat preconditioning, acetylsalicylic acid, streptozotocin-induced diabetes, heart, carbohydrate-related enzymes and substrates

© 2022 Mirsada Dervisevik et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
heat preconditioning, acetylsalicylic acid, streptozotocin-induced diabetes, heart, carbohydrate-related enzymes and substrates