<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The present study was aimed to evaluate hematological and oxidative stress parameters in domestic dogs infested naturally (n=10) by <italic>Rhipicephalus</italic> sp. to compare with non-infested dogs (n=10). All blood samples were collected from brachial vein into tubes EDTA for the hematological analysis such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (HGB) and platelets (PLT). Serum was rapidly separated after centrifugation and stored at -20 °C until it was used for malondialdehyde (MDA) and 2,2’-Azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition measurements. HGB in non-infested dogs was significantly higher than in infested dogs (P&lt;0.05). There was no significant difference in RBCs, WBCs and PLT between both groups (P>0.05). The mean of MDA concentration was high in infested dogs (0.92±0.62 nmol/ml) compared to non-infested dogs (0.75±0.25 nmol/ml). On the other hand, the percentage of ABTS inhibition was similar in both groups (P=0.71). High tick number seems significantly affected WBCs (P&lt;0.0001) and HGB (P&lt;0.001) in infested dogs. Concerning oxidative status, there was no significant differences (P>0.05) between low and high infested dogs, neither in the amount of MDA nor in the ABTS inhibition. In conclusion, infested dogs induced RBCs alterations, which coincided with the oxidative damage, as evidenced by MDA serum levels. Also, there was a relationship between the tick number in infested dogs and the hematological parameters.</p></abstract>

The present study was aimed to evaluate hematological and oxidative stress parameters in domestic dogs infested naturally (n=10) by Rhipicephalus sp. to compare with non-infested dogs (n=10). All blood samples were collected from brachial vein into tubes EDTA for the hematological analysis such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (HGB) and platelets (PLT). Serum was rapidly separated after centrifugation and stored at -20 °C until it was used for malondialdehyde (MDA) and 2,2’-Azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition measurements. HGB in non-infested dogs was significantly higher than in infested dogs (P<0.05). There was no significant difference in RBCs, WBCs and PLT between both groups (P>0.05). The mean of MDA concentration was high in infested dogs (0.92±0.62 nmol/ml) compared to non-infested dogs (0.75±0.25 nmol/ml). On the other hand, the percentage of ABTS inhibition was similar in both groups (P=0.71). High tick number seems significantly affected WBCs (P<0.0001) and HGB (P<0.001) in infested dogs. Concerning oxidative status, there was no significant differences (P>0.05) between low and high infested dogs, neither in the amount of MDA nor in the ABTS inhibition. In conclusion, infested dogs induced RBCs alterations, which coincided with the oxidative damage, as evidenced by MDA serum levels. Also, there was a relationship between the tick number in infested dogs and the hematological parameters.

Hematological and Oxidative Status Parameters in Domestic Dogs Naturally Infested by <italic>Rhipicephalus</italic> Sp.

Department of Environment Biological Sciences, Faculty of Nature and Life Sciences

Department of Agronomic and Biotechnological Sciences, Faculty of Nature and Life Sciences

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hematological and Oxidative Status Parameters in Domestic Dogs Naturally Infested by  Sp.

The present study was aimed to evaluate hematological and oxidative stress parameters in domestic dogs infested naturally (n=10) by Rhipicephalus sp. to compare with non-infested dogs (n=10). All blood samples were collected from brachial vein into tubes EDTA for the hematological analysis such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (HGB) and platelets (PLT). Serum was rapidly separated after centrifugation and stored at -20 °C until it was used for malondialdehyde (MDA) and 2,2’-Azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition measurements. HGB in non-infested dogs was significantly higher than in infested dogs (P0.05). The mean of MDA concentration was high in infested dogs (0.92±0.62 nmol/ml) compared to non-infested dogs (0.75±0.25 nmol/ml). On the other hand, the percentage of ABTS inhibition was similar in both groups (P=0.71). High tick number seems significantly affected WBCs (P0.05) between low and high infested dogs, neither in the amount of MDA nor in the ABTS inhibition. In conclusion, infested dogs induced RBCs alterations, which coincided with the oxidative damage, as evidenced by MDA serum levels. Also, there was a relationship between the tick number in infested dogs and the hematological parameters.

The present study was aimed to evaluate hematological and oxidative stress parameters in domestic dogs infested naturally (n=10) by Rhipicephalus sp. to...

Hematological and Oxidative Status Parameters in Domestic Dogs Naturally Infested by Rhipicephalus Sp.

Published Online: Oct 15, 2020

Page range: 103 - 110

Received: Feb 20, 2020

Accepted: Jun 09, 2020

DOI: https://doi.org/10.2478/macvetrev-2020-0022

Keywords
sp., hematological parameters, oxidative status, dogs

© 2020 Rosa Kebbi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hematological and Oxidative Status Parameters in Domestic Dogs Naturally Infested by Rhipicephalus Sp.

Published Online: Oct 15, 2020

Page range: 103 - 110

Received: Feb 20, 2020

Accepted: Jun 09, 2020

DOI: https://doi.org/10.2478/macvetrev-2020-0022

Keywordssp., hematological parameters, oxidative status, dogs

© 2020 Rosa Kebbi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
sp., hematological parameters, oxidative status, dogs