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Cultivation of Bartonella henselae in an Ixodes ricinus cell line to assess this tick as a potential reservoir of the bacterium

Violetta Zając

Violetta Zając

Department of Health Biohazards and Parasitology, Institute of Rural HealthLublin, Poland
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Zając, Violetta
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Jacek Sroka

Jacek Sroka

Department of Parasitology and Invasive Diseases, Bee Diseases and Aquatic Animal Diseases, National Veterinary Research InstitutePuławy, Poland
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Sroka, Jacek
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Ewa Bilska-Zając

Ewa Bilska-Zając

Department of Parasitology and Invasive Diseases, Bee Diseases and Aquatic Animal Diseases, National Veterinary Research InstitutePuławy, Poland
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Bilska-Zając, Ewa
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Angelina Wójcik-Fatla

Angelina Wójcik-Fatla

Department of Health Biohazards and Parasitology, Institute of Rural HealthLublin, Poland
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Wójcik-Fatla, Angelina
  
Aug 31, 2025
Cultivation of Bartonella henselae in an Ixodes ricinus cell line to assess this tick as a potential reservoir of the bacterium's Cover Image
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Published Online: Aug 31, 2025
Received: Jan 31, 2025
Accepted: Aug 27, 2025
DOI: https://doi.org/10.2478/jvetres-2025-0045
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© 2025 Violetta Zając et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1.

Experimental design schematic. d.p.i. – days post infection; B. henselae – Bartonella henselae; IRE/CTVM19 – Ixodes ricinus embryonic cell line
Experimental design schematic. d.p.i. – days post infection; B. henselae – Bartonella henselae; IRE/CTVM19 – Ixodes ricinus embryonic cell line

Fig. 2.

Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of monolayer cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection
Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of monolayer cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection

Fig. 3.

Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of supernatant cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection. * – statistically significant (P-value < 0.01)
Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of supernatant cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection. * – statistically significant (P-value < 0.01)
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Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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