Whole genome sequencing and analysis of a goose-derived Mycoplasma gallisepticum in Guangdong Province, China
Yuanyuan Zhou
Orcid profile, Shuti Song
Orcid profile, Weihuo Li
Orcid profile, Yixin Jia
Orcid profile, Yangshuo Li
Orcid profile, Jingyi Liang
Orcid profile, Zhaofeng Yao
Orcid profile and 
Published Online: Nov 28, 2024
Page range: 497 - 508
Received: Apr 18, 2024
Accepted: Nov 21, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0065
Keywords
© 2024 Yuanyuan Zhou et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Material and Methods
A strain designated MG-GD01/22 was isolated from the air-sac tissues of five geese with chronic respiratory disease on a Guangdong goose farm. Its pathogenicity was assessed, antimicrobial susceptibility tests were performed using agar dilution, and its total DNA was extracted for whole-genome sequencing and gene function annotation with second- and third-generation sequencing technologies. The homology of the 16S ribosomal RNA (rRNA) region was analysed and a phylogenetic tree was constructed, as was an evolutionary tree of the
Results
The isolate produced “fried egg” colonies and was pathogenic to goslings. It was resistant to enrofloxacin, danofloxacin and spectinomycin and susceptible to valnemulin, tilmicosin, tylosin, acetylisovaleryltylosin tartrate and tiamulin. The genome analysis revealed 1,666 coding genes. Gene database annotation identified 25 virulence-related genes, 22 drug resistance–related genes, 13 pathogen-host–interaction genes and 9 carbohydrate-active enzyme genes. The isolate exhibited 99.9% homology to the MG S6 strain by its 16S rRNA, while the
Conclusion
This study offers theoretical references for the diagnosis, prevention and treatment of MG in geese in the Guangdong region.