Molecular survey and genetic diversity of Plasmodium sp. infesting domestic poultry in northeastern Thailand
, , , , and | Mar 23, 2024
About this article
Published Online: Mar 23, 2024
Page range: 101 - 108
Received: Sep 06, 2023
Accepted: Feb 19, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0010
Keywords
© 2024 Wasupon Chatan et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1.
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Fig. 3.
Lineages of 37 cytochrome b gene sequences from backyard poultry in Thailand with matches to MalAvi database and National Center for Biotechnology Information (NCBI) GenBank database sequences
| Lineage names | Parasites | Sample IDs | NCBI GenBank accession No. | Closest sequences in NCBI GenBank (% similarity) |
|---|---|---|---|---|
| ACCBAD01 | D3, D5, D11, D34, D38, D42, D43, D51, D53, T4, T6, T13, T15, T26, T34, T17 | OR341180 | MK390829.1 (100%), JN639001.1 (100%) | |
| ORW1 | D36, T11, T12, T14, T16, T18, T21, T22, T25, T27, T29, T31, T30, T32 | OR341179 | EF552403.1 (100%), KJ145050.1 (100%), KJ396632.1 (100%) | |
| FANTAIL01 | G5 | OR341184 | AY714196.1 (100%), HF543648.1 (100%) | |
| D1 | OR341178 | MK390829.1 (99.78%), JN639001.1 (99.78%) | ||
| GALLUS01 | T2, T7 | OR341181 | LC506179.1 (96.63-100%), LN835294.1 (96.63-100%) | |
| T20, T28 | OR341183 | LC506179.1 (99.77%), LN835294.1 (99.77%) | ||
| GALLUS02 | T9 | OR341182 | KU248845.1 (99.56%), KT290918.1 (99.56%) |
Prevalence of Plasmodium spp. infections in backyard poultry
| Locations (Provinces) | Host | Prevalence of |
|||
|---|---|---|---|---|---|
| Common name | Scientific name | Microscopic examination (%) | PCR examination (%) | ||
| Nongkhai | Domestic duck | 1/30 (3.3) | 4/30 (13.3) | ||
| Domestic turkey | 1/10 (10) | 5/10 (50) | |||
| Maha Sarakham | Swan goose | 0/11 (0) | 1/11 (9.1) | ||
| Domestic duck | 2/30 (6.7) | 7/30 (23.3) | |||
| Domestic turkey | 7/26 (26.9) | 20/26 (76.9) | |||
| Swan goose | 0/9 (0) | 0/9 (0) | - | ||
| Total | 11/116 (9.5) | 37/116 (31.9) | |||
Association among Plasmodium spp. infections with PCV levels
| Host | Parasites | Levels of PCV (%) (mean ± SD) | 95% CI of average PCV value (%) | P-value | Normal range (24) | |
|---|---|---|---|---|---|---|
| Common name | Scientific name | |||||
| Duck | infected (n = 11) | 34.91 ± 4.87 | 31.64-38.18 | 0.62 | 36-58% | |
| uninfected (n = 49) | 33.83 ± 6.68 | 31.89-35.77 | ||||
| Turkey | infected (n = 25) | 38.92 ± 5.50 | 36.59-41.24 | 0.07 | 36-41% | |
| uninfected (n = 11) | 35.09 ± 5.82 | 31.18-39.00 | ||||
| Geese | infected (n = 1) | 47.00 | N/A | N/A | 38-58% | |
| uninfected (n = 19) | 41.10 ± 3.03 | 38.93-43.27 | ||||