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Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland

Sebastian Smulski

Sebastian Smulski

Department of Internal Diseases and Diagnostics, Poznan University of Life SciencesPoznan, Poland
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Smulski, Sebastian
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Marcin Pszczoła

Marcin Pszczoła

Department of Genetics and Animal Breeding, Poznan University of Life SciencesPoznan, Poland
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Pszczoła, Marcin
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Monika Stachowiak

Monika Stachowiak

Department of Genetics and Animal Breeding, Poznan University of Life SciencesPoznan, Poland
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Stachowiak, Monika
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Adrianna Bilińska

Adrianna Bilińska

Department of Genetics and Animal Breeding, Poznan University of Life SciencesPoznan, Poland
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Bilińska, Adrianna
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Izabela Szczerbal

Izabela Szczerbal

Department of Genetics and Animal Breeding, Poznan University of Life SciencesPoznan, Poland
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Szczerbal, Izabela
  
Dec 19, 2023
Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland's Cover Image
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Published Online: Dec 19, 2023
Page range: 583 - 591
Received: Jun 15, 2023
Accepted: Oct 27, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0062
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© 2023 Sebastian Smulski et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Correlation between log(SCC) and concentrations of miR-21a, miR-92a and miR-146a in healthy and mastitic milk samples. The grey area below the figures indicates the 95% confidence interval. Low – somatic cell count < 200,000/mL; Medium – 200,000/mL ≤ somatic cell count < 1,000,000/mL; High – somatic cell count ≥ 1,000,000/mL
Correlation between log(SCC) and concentrations of miR-21a, miR-92a and miR-146a in healthy and mastitic milk samples. The grey area below the figures indicates the 95% confidence interval. Low – somatic cell count < 200,000/mL; Medium – 200,000/mL ≤ somatic cell count < 1,000,000/mL; High – somatic cell count ≥ 1,000,000/mL

Fig. 2.

Correlation between log(SCC) and the relative expression levels of miR-21a and miR-146a in healthy and mastitic milk samples. The grey area below the figures indicates the 95% confidence interval. Low – somatic cell count < 200,000/mL; Medium – 200,000/mL ≤ somatic cell count < 1,000,000/mL; High – somatic cell count ≥ 1,000,000/mL
Correlation between log(SCC) and the relative expression levels of miR-21a and miR-146a in healthy and mastitic milk samples. The grey area below the figures indicates the 95% confidence interval. Low – somatic cell count < 200,000/mL; Medium – 200,000/mL ≤ somatic cell count < 1,000,000/mL; High – somatic cell count ≥ 1,000,000/mL

Fig. 3.

Relative expression levels of miR-21a and miR-146a in three groups based on somatic cell counts and presence of a mastitoge. a, b – significant differences (P-value < 0.05)
Relative expression levels of miR-21a and miR-146a in three groups based on somatic cell counts and presence of a mastitoge. a, b – significant differences (P-value < 0.05)

Prevalence of pathogens isolated from mastitic-quarter milk samples

Pathogen n Prevalence (%)
Streptococcus uberis 6 9.23
Streptococcus dysgalactiae 1 1.54
Staphylococcus aureus 1 1.54
Coagulase-negative staphylococci 4 6.15
Escherichia coli 1 1.54
Klebsiella spp. 2 3.08
No growth 33 50.77
Contamination 17 26.15

Performance of the K-nearest neighbour regression model, by somatic cell count class in healthy and mastitic milk samples

Parameter Three-class analysis Two-class analysis
Low Medium High Low
Sensitivity 0.67 0.67 0.82 0.98
Specificity 0.85 0.75 0.98 0.95
Positive predictive value 0.63 0.62 0.95 0.98
Negative predictive value 0.87 0.79 0.91 0.95
Balanced accuracy 0.76 0.71 0.90 0.97
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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