Comparison of automatic methods MALDI-TOF, VITEK2 and manual methods for the identification of intestinal microbial communities on the example of samples from alpacas (Vicugna pacos )
Published Online: Sep 20, 2023
Page range: 361 - 372
Received: Mar 23, 2023
Accepted: Aug 22, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0051
Keywords
© 2023 Joanna Pławińska-Czarnak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Universally, in microbiological diagnostics the detection of live bacteria is essential. Rapid identification of pathogens enables appropriate remedial measures to be taken. The identification of many bacteria simultaneously facilitates the determination of the characteristics of the accompanying microbiota and/or the microbiological complexity of a given environment.
Material and Methods
The effectiveness of the VITEK2 Compact automated microbial identification system and matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS), analytical profile index (API) and Remel RapID tests were compared in identification of bacteria isolated from the alpaca gastrointestinal tract.
Results
Most isolates were Gram-positive, such as
Conclusion
MALDI-TOF MS enabled the identification of pathogens and opportunistic pathogens from the alpaca gut which may represent a high risk to human and animal health.