Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage

Marcjanna Wrzecińska; Alicja Kowalczyk; Ewa Czerniawska-Piątkowska; Władysław Kordan; Jose Pedro Araujo

Open Access

Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage

Marcjanna Wrzecińska

Alicja Kowalczyk

Ewa Czerniawska-Piątkowska

Władysław Kordan

and

Jose Pedro Araujo

| Sep 20, 2023

Journal of Veterinary Research

Volume 67 (2023): Issue 3 (September 2023)

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Page range: 415 - 425

Received: Feb 24, 2023

Accepted: Jul 13, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0043

Keywords
Polish Holstein-Friesian cow, haematological profile, blood examination, pregnant cows, reproduction

© 2023 Marcjanna Wrzecińska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The division of animals into groups according to the length of pregnancy
Group C00 – control group of non-pregnant heifers; Group C0 – control group of non-pregnant cows; Group T1 – experimental heifers at day of pregnancy (dop) ≤ 28; Group T2 – experimental heifers at dop ≥ 29–dop < 45; Group T3 – experimental cows at dop ≥ 29–dop ≤ 50 — The division of animals into groups according to the length of pregnancy Group C00 – control group of non-pregnant heifers; Group C0 – control group of non-pregnant cows; Group T1 – experimental heifers at day of pregnancy (dop) ≤ 28; Group T2 – experimental heifers at dop ≥ 29–dop < 45; Group T3 – experimental cows at dop ≥ 29–dop ≤ 50

Transrectal ultrasound image showing late embryo development at around the 29th day of pregnancy

Transrectal ultrasound image showing late embryo development at around the 30th day of pregnancy

Transrectal ultrasound image showing late embryo development at around the 31st day of pregnancy

Transrectal ultrasound image showing late embryo development at around the 32nd day of pregnancy

Transrectal ultrasound image showing late embryo development at around the 35th day of pregnancy

Transrectal ultrasound image showing late embryo development at around the 37th day of pregnancy

Transrectal ultrasound image showing late embryo development at around the 39th day of pregnancy

Comparison of average haematological parameters of Polish black-and-white Holsteina-Friesian cattle setting heifers against pregnant cows

Parameter	H $\bar{x} \pm SD$ $$\overline {\rm{x}} \pm {\rm{SD}}$$	C $\bar{x} \pm SD$ $$\overline {\rm{x}} \pm {\rm{SD}}$$
WBC (10³/μL)	9.11^a ± 2.85	6.74^b ± 1.76
GRA (10³/μL)	4.35 ± 2.17**	4.02 ± 1.30
GRA% (%)	46.49^A ± 9.57**	58.80^B ± 5.66*
LYM (10³/μL)	4.09^A ± 1.27**	2.20^B ± 0.49
LYM% (%)	46.10^A ± 9.60**	33.45^B ± 4.82
MON (10³/μL)	0.67 ± 0.23	0.51 ± 0.21
MON% (%)	7.41 ± 1.14	7.75 ± 1.98
RDW (10³/μL)	19.96 ± 1.30	19.44 ± 1.66
RBC (10⁶/μL)	8.46^a ± 0.94**	6.56^b ± 0.49*
HGB (g/dL)	12.21^A ± 1.67*	9.95^B ± 0.98*
MCH (pg)	14.44 ± 1.01	15.19 ± 1.16
MCHC (g/dL)	35.08 ± 0.91*	33.95 ± 0.57
MCV (μm³)	41.20^A ± 3.09	44.72^B ± 3.06*
MPV (μm³)	6.76 ± 0.77	6.27 ± 0.62
PCT (%)	0.14 ± 0.07	0.26 ± 0.13
PDW (%)	40.59^A ± 11.38*	20.25^B ± 4.03*
PLT (10³/μL)	206.52^A ± 110.26**	402.41^B ± 224.99**
HCT (%)	34.18^A ± 6.05**	29.31^B ± 2.62

Average values of haematological parameters of Polish black-and-white Holstein-Friesian cattle, comparing those of non-pregnant heifers to those of pregnant cows grouped by length of pregnancy

Parameter	Group 1 $\bar{x} \pm SD$ $$\overline {\rm{x}} \pm {\rm{SD}}$$	Group 2 $\bar{x} \pm SD$ $$\overline {\rm{x}} \pm {\rm{SD}}$$	Group 3 $\bar{x} \pm SD$ $$\overline {\rm{x}} \pm {\rm{SD}}$$
WBC (10³/μL)	8.34 ± 2.03**	7.30 ± 2.38**	9.66 ± 4.46
GRA (10³/μL)	4.26 ± 1.04	3.72 ± 1.31**	5.34 ± 3.86
GRA% (%)	51.76 ± 8.86**	51.70 ± 11.04**	50.86 ± 12.66
LYM (10³/μL)	3.43 ± 1.38**	3.07 ± 1.50*	3.58 ± 1.08
LYM% (%)	40.63 ± 8.85**	40.91 ± 11.02**	41.34 ± 12.40
MON (10³/μL)	0.64 ± 0.23	0.52 ± 0.15	0.75 ± 0.33
MON% (%)	7.61 ± 1.86	7.40 ± 1.42	7.80 ± 0.55
RDW (10³/μL)	19.42 ± 1.06	19.88 ± 1.72	20.36 ± 1.75
RBC (10⁶/μL)	7.63 ± 1.10**	7.44 ± 1.46**	8.40 ± 0.58
HGB (g/dL)	11.06 ± 1.58**	11.40 ± 2.32*	11.59 ± 0.54
MCH (pg)	14.51 ± 0.84	15.36 ± 1.12	13.85 ± 1.13
MCHC (g/dL)	34.34 ± 0.74*	34.60 ± 1.05	35.45 ± 0.93
MCV (μm³)	42.28 ± 2.58 *	44.44 ± 3.40	39.13 ± 3.56
MPV (μm³)	6.54 ± 0.81	6.50 ± 0.73	6.83 ± 0.66
PCT (%)	0.18 ± 0.11	0.19 ± 0.13	0.17 ± 0.08
PDW (%)	31.96 ± 13.08**	29.53 ± 13.71**	42.23 ± 11.63
PLT (10³/μL)	290.17 ± 191.98**	300.00 ± 219.29**	248.88 ± 117.54
HCT (%)	32.15 ± 4.11**	31.95 ± 7.52**	32.73± 1.92

Reference values for haematological parameters of dairy cattle

	Reference value
WBC	4.0–12.0 (10³/μL)
GRA	0.6–4.0 (10³/μL)
GRA%	15.0–50.0 (%)
LYM	2.5–7.5 (10³/μL)
LYM%	45.0–75.0 (%)
MON	0.1–0.8 (10³/μL)
MON%	2.0–7.0 (%)
RDW	16.0–24.0 (10³/μL)
RBC	5.0–10.0 (10⁶/μL)
HGB	8.0–15.0 (g/dL)
MCH	11.0–18.0 (pg)
MCHC	30.0–37.0 (g/dL)
MCV	37.0–60.0 (μm³)
MPV	5.5–7.5 (μm³)
PCT	0.250–0.650 (%)
PDW	10.0–20.0 (%)
PLT	150–730 (10³/μL)
HCT	24.0–46.0 (%)

Pearson’s correlation values for haematological parameters of cows’ blood

	WBC	RBC	HGB	HCT	PLT	LYM	MON	GRA	LYM%
WBC	1	0.64145**	0.58184**	0.55768**	0.12123ns	0.71413**	0.83838**	0.87833ns	0.03639ns
RBC		1	0.94734**	0.90814**	0.12114ns	0.68151**	0.44398**	0.35080**	0.44345**
HGB			1	0.95365**	0.03649ns	0.66429**	0.32751*	0.26443*	0.48052**
HCT				1	0.06239ns	0.58727**	0.28291*	0.28316*	0.37719*
PLT					1	0.04063ns	0.09542ns	0.13425ns	0.15948ns
LYM						1	0.60442**	0.29617*	0.64392**
MON							1	0.69739**	0.00841ns
GRA								1	0.48615**
LYM%									1
	MON%	GRA%	MCV	MCH	MCHC	RDW	MPV	PCT	PDW
WBC	0.23363*	0.07747ns	-	-	-	0.36385*	0.02595ns	0.12563ns	0.41233**
RBC	0.30012*	0.37933*	0.45821**	0.20889*	0.64494**	0.25965*	0.01210ns	0.12181ns	0.56941**
HGB	0.41980**	0.39406**	0.19525*	0.08335ns	0.73003**	0.25594*	0.01719ns	0.03862ns	0.45478**
HCT	0.44251**	0.28904*	0.17111ns	0.05845ns	0.59783**	0.23689*	0.07609ns	0.06153ns	0.36950*
PLT	0.04885ns	0.14702ns	0.17235ns	0.17466ns	0.07183ns	0.03623ns	0.15619ns	0.99610**	0.30944*
LYM	0.15027ns	0.60204**	0.27350*	0.09556ns	0.45777**	0.28579*	0.06002ns	0.03566ns	0.40430**
MON	0.27067*	0.04035ns	0.38371*	0.26092*	0.28212*	0.36855*	0.09669ns	0.09607ns	0.31521*
GRA	0.27286*	0.52385**	0.24411*	0.12630ns	0.30521*	0.29278*	0.01533ns	0.13912ns	0.21823*
LYM%	0.03879ns	0.98379**	0.13328ns	0.03809ns	0.23664*	0.07820ns	0.11351ns	0.15558ns	0.30458*
	MON%	GRA%	MCV	MCH	MCHC	RDW	MPV	PCT	PDW
MON%	1	0.21733*	0.30243*	0.43501**	0.46124**	0.06582ns	0.10003ns	0.03840ns	0.07662ns
GRA%		1	0.18446ns	0.11527ns	0.14839ns	0.08820ns	0.12886ns	0.14511ns	0.28382*
MCV			1	0.93347**	0.02221ns	0.23940*	0.05386ns	0.16434ns	0.45209**
MCH				1	0.37543*	0.16699ns	0.03119ns	0.16664ns	0.28139*
MCHC					1	0.16761ns	0.05376ns	0.07050ns	0.36066*
RDW						1	0.05475ns	0.03287ns	0.20184*
MPV							1	0.09250ns	0.36006*
PCT								1	0.28095*
PDW									1

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Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage

Published Online: Sep 20, 2023

Page range: 415 - 425

Received: Feb 24, 2023

Accepted: Jul 13, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0043

Keywords
Polish Holstein-Friesian cow, haematological profile, blood examination, pregnant cows, reproduction

© 2023 Marcjanna Wrzecińska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

Fig. 8.

Comparison of average haematological parameters of Polish black-and-white Holsteina-Friesian cattle setting heifers against pregnant cows

Average values of haematological parameters of Polish black-and-white Holstein-Friesian cattle, comparing those of non-pregnant heifers to those of pregnant cows grouped by length of pregnancy

Reference values for haematological parameters of dairy cattle

Pearson’s correlation values for haematological parameters of cows’ blood

Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage

Published Online: Sep 20, 2023

Page range: 415 - 425

Received: Feb 24, 2023

Accepted: Jul 13, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0043

KeywordsPolish Holstein-Friesian cow, haematological profile, blood examination, pregnant cows, reproduction

© 2023 Marcjanna Wrzecińska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

Fig. 8.

Comparison of average haematological parameters of Polish black-and-white Holsteina-Friesian cattle setting heifers against pregnant cows

Average values of haematological parameters of Polish black-and-white Holstein-Friesian cattle, comparing those of non-pregnant heifers to those of pregnant cows grouped by length of pregnancy

Reference values for haematological parameters of dairy cattle

Pearson’s correlation values for haematological parameters of cows’ blood

Keywords
Polish Holstein-Friesian cow, haematological profile, blood examination, pregnant cows, reproduction