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Retrospective study on equine viral abortions in Poland between 1999 and 2022

Karol Stasiak

Karol Stasiak

Department of Virology, National Veterinary Research InstitutePuławy, Poland
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Stasiak, Karol
, 
Wojciech Socha

Wojciech Socha

Department of Virology, National Veterinary Research InstitutePuławy, Poland
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Socha, Wojciech
 and 
Jerzy Rola

Jerzy Rola

Department of Virology, National Veterinary Research InstitutePuławy, Poland
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Rola, Jerzy
  
Jun 16, 2023
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Published Online: Jun 16, 2023
Page range: 155 - 160
Received: Sep 06, 2022
Accepted: Mar 16, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0019
Keywords
© 2023 Karol Stasiak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Geographical distribution and number of cases of equine abortion in Poland 1999–2022 Brackets enclose the number of EHV-1 cases
Geographical distribution and number of cases of equine abortion in Poland 1999–2022 Brackets enclose the number of EHV-1 cases

Fig. 2.

Numbers of abortion cases caused by EHV-1 in consecutive years between 1999 and 2022
Numbers of abortion cases caused by EHV-1 in consecutive years between 1999 and 2022

The numbers and percentages of abortion associated with EHV-1, -4 and EAV infections between 1999 and 2022

Perioda EHV-1 EHV-4 EAV
n/N % n/N % n/N %
1999–2012 20/63 31.7 0/63 0.0 0/43 0.0
2013–2022 69/117 59.0 0/117 0.0 0/48 0.0
All 89/180 49.4 0/180 0.0 0/91 0.0

Primers and probes used for detection of equine herpesvirus 1 (EHV-1), equine herpesvirus 4 (EHV-4) and equine arteritis virus (EAV) between 1999 and 2022

Assay Virus Region Primers / probes (5′ to 3′) Size (bp) Reference
Forward: CATGTCAACGCACTCCCA
EHV-1 Reverse: GGGTCGGGCGTTTCTGT 63
gB Probe: FAM-CCCTACGCTGCTCC-TAMRA (9)
Forward: GGGCTATTGGATTACAGCGAGAT
QuantitativePCRs EHV-4 Reverse: TAGAATCGGAGGGCGTGAAG 58
Probe: VIC-CAGCGCCGTAACCAG-TAMRA
Forward: GGCGACAGCCTACAAGTCACA
EAV N Reverse: CGGCATCTGCAGTGAGTGA 204 (4)
Probe: FAM-TTGCGGACCCGCATCTGACCAA-TAMRA
EHV-1/-4 Forward: CTTGTGAGATCTAACCGCAC
EHV-1 gB Reverse: GCGTTATAGCTATCACGTCC 459 (EHV-1) (13)
ConventionalPCRs EHV-4 Reverse: CCTGCATAATGACAGCAGTG 942 (EHV-4)
EAV N Forward: TCGATGGCGTCAAGA 395 (7)
Reverse: GGTTCCTGGGTGGCTAATAACTACTTCAAC
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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