Comparison of various diagnostic techniques for the detection of Blastocystis spp. and its molecular characterisation in farm animals in the United Arab Emirates
Published Online: Mar 28, 2023
Page range: 93 - 98
Received: Jul 21, 2022
Accepted: Mar 09, 2023
DOI: https://doi.org/10.2478/jvetres-2023-0017
Keywords
© 2023 A. ElBakri et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Material and Methods
Ninety-seven faecal samples comprised of 69 from sheep, 12 from cows and 16 from camels were submitted to DNA extraction, PCR and sequencing.
Results
Fifteen (15.5%) samples were positive by PCR, twelve of which were confirmed by sequencing. Using PCR as a comparison standard, the sensitivity and specificity of the direct wet-mount, modified acid-fast staining, trichrome staining and
Conclusion
The study corroborated previous data indicating that sheep are the natural hosts for ST10. No zoonotic subtypes nor mixed-subtype colonisation were found. The report also confirmed the superiority of trichrome staining in detecting