<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2022-0009_s_005">
<h3>Introduction</h3>
<p>Hypoxia is a common pathological condition after spinal cord injury. Oestrogen-related receptor alpha (ERRα), as a key regulator of energy metabolism and mitochondrial functions, plays an important role in maintaining cell homeostasis. However, its role in hypoxic spinal microglia has not been fully elaborated. This study investigated the receptor’s activity when these cells are hypoxic and used as an <italic>in vitro</italic> model.</p>
</sec>
<sec id="j_jvetres-2022-0009_s_006">
<h3>Material and Methods</h3>
<p>In this study, microglia (BV2) were exposed to cobalt chloride as a hypoxic model, and the inverse agonist of ERRα, XCT790, and pyrido[1,2-α]-pyrimidin-4-one were used to regulate the expression of the receptor to explore the ERRα-related mechanisms involved in hypoxic spinal cord injury (SCI).</p></sec>
<sec id="j_jvetres-2022-0009_s_007">
<h3>Results</h3>
<p>ERRα promoted autophagy in BV2 cells and inhibited the activation of the p38 mitogen-activated protein kinase (MAPK) pathway and the expression of anti-inflammatory factors under hypoxic conditions. It also promoted the expression of fibronectin type III domain containing protein 5 (FNDC5).</p></sec>
<sec id="j_jvetres-2022-0009_s_008">
<h3>Conclusion</h3>
<p>When a hypoxic SCI occurs, ERRα may maintain the homeostasis of spinal cord nerve cells by regulating autophagy and the p38MAPK/nuclear factor-kappa B cell and FNDC5/brain-derived neurotrophic factor signalling pathways, which are beneficial to the recovery of these cells.</p>
</sec>
</abstract>

Introduction
Hypoxia is a common pathological condition after spinal cord injury. Oestrogen-related receptor alpha (ERRα), as a key regulator of energy metabolism and mitochondrial functions, plays an important role in maintaining cell homeostasis. However, its role in hypoxic spinal microglia has not been fully elaborated. This study investigated the receptor’s activity when these cells are hypoxic and used as an in vitro model.


Material and Methods
In this study, microglia (BV2) were exposed to cobalt chloride as a hypoxic model, and the inverse agonist of ERRα, XCT790, and pyrido[1,2-α]-pyrimidin-4-one were used to regulate the expression of the receptor to explore the ERRα-related mechanisms involved in hypoxic spinal cord injury (SCI).

Results
ERRα promoted autophagy in BV2 cells and inhibited the activation of the p38 mitogen-activated protein kinase (MAPK) pathway and the expression of anti-inflammatory factors under hypoxic conditions. It also promoted the expression of fibronectin type III domain containing protein 5 (FNDC5).

Conclusion
When a hypoxic SCI occurs, ERRα may maintain the homeostasis of spinal cord nerve cells by regulating autophagy and the p38MAPK/nuclear factor-kappa B cell and FNDC5/brain-derived neurotrophic factor signalling pathways, which are beneficial to the recovery of these cells.

Estrogen-related receptor α (ERRα) functions in the hypoxic injury of microglial cells

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Hypoxia is a common pathological condition after spinal cord injury. Oestrogen-related receptor alpha (ERRα), as a key regulator of energy...

Estrogen-related receptor α (ERRα) functions in the hypoxic injury of microglial cells

Published Online: Mar 10, 2022

Page range: 131 - 140

Received: Jul 17, 2021

Accepted: Feb 15, 2022

DOI: https://doi.org/10.2478/jvetres-2022-0009

Keywords
spinal cord injury, microglia, oestrogen-related receptor alpha, cobalt chloride, nuclear factor-kappa B

© 2022 C.Y. Deng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Estrogen-related receptor α (ERRα) functions in the hypoxic injury of microglial cells

Published Online: Mar 10, 2022

Page range: 131 - 140

Received: Jul 17, 2021

Accepted: Feb 15, 2022

DOI: https://doi.org/10.2478/jvetres-2022-0009

Keywordsspinal cord injury, microglia, oestrogen-related receptor alpha, cobalt chloride, nuclear factor-kappa B

© 2022 C.Y. Deng et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
spinal cord injury, microglia, oestrogen-related receptor alpha, cobalt chloride, nuclear factor-kappa B