<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2021-0045_s_005">
<h3>Introduction</h3>
<p>Because minipig skin is similar to human skin in anatomy and physiology, establishing an atopic dermatitis (AD) minipig model seems meaningful.</p></sec>
<sec id="j_jvetres-2021-0045_s_006">
<h3>Material and Methods</h3>
<p>We applied 1-fluoro-2,4-dinitrobenzene (DNFB) or ovalbumin onto the back skin of five Yucatan minipigs aged 8–10 months and 19 kg in median weight. Two minipigs with the same parameters served as controls.</p></sec>
<sec id="j_jvetres-2021-0045_s_007">
<h3>Results</h3>
<p>Both DNFB and ovalbumin mediated epithelial hyperplasia, spongiosis, and immune cell infiltration in the dermis, which is a typical histopathological feature of AD. Moreover, AD upregulated the Th1- and Th2-related cytokine expressions in DNFB- or in ovalbumin-treated skin. Notably, AD-induced minipigs exhibited greater cytokine serum concentrations.</p></sec>
<sec id="j_jvetres-2021-0045_s_008">
<h3>Conclusion</h3>
<p>Histopathological finding and cytokine analysis revealed that DNFB or ovalbumin mediates AD. However, ovalbumin-treated minipig is a more reliable and precise AD model owing to the DNFB-induced severe skin damage. In summary, ovalbumin-treated skin shows similar AD as human in histopathological and molecular analysis.</p></sec>
</abstract>

Introduction
Because minipig skin is similar to human skin in anatomy and physiology, establishing an atopic dermatitis (AD) minipig model seems meaningful.

Material and Methods
We applied 1-fluoro-2,4-dinitrobenzene (DNFB) or ovalbumin onto the back skin of five Yucatan minipigs aged 8–10 months and 19 kg in median weight. Two minipigs with the same parameters served as controls.

Results
Both DNFB and ovalbumin mediated epithelial hyperplasia, spongiosis, and immune cell infiltration in the dermis, which is a typical histopathological feature of AD. Moreover, AD upregulated the Th1- and Th2-related cytokine expressions in DNFB- or in ovalbumin-treated skin. Notably, AD-induced minipigs exhibited greater cytokine serum concentrations.

Conclusion
Histopathological finding and cytokine analysis revealed that DNFB or ovalbumin mediates AD. However, ovalbumin-treated minipig is a more reliable and precise AD model owing to the DNFB-induced severe skin damage. In summary, ovalbumin-treated skin shows similar AD as human in histopathological and molecular analysis.

A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model"}

Introduction
Because minipig skin is similar to human skin in anatomy and physiology, establishing an atopic dermatitis (AD) minipig model seems...

A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model

Published Online: Aug 19, 2021

Page range: 307 - 313

Received: Feb 18, 2021

Accepted: Aug 02, 2021

DOI: https://doi.org/10.2478/jvetres-2021-0045

Keywords
atopic dermatitis, Yucatan minipig, 1-fluoro-2,4-dinitrobenzene, ovalbumin

© 2021 Y.K. Kim et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A pilot study to establish an ovalbumin-induced atopic dermatitis minipig model

Published Online: Aug 19, 2021

Page range: 307 - 313

Received: Feb 18, 2021

Accepted: Aug 02, 2021

DOI: https://doi.org/10.2478/jvetres-2021-0045

Keywordsatopic dermatitis, Yucatan minipig, 1-fluoro-2,4-dinitrobenzene, ovalbumin

© 2021 Y.K. Kim et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
atopic dermatitis, Yucatan minipig, 1-fluoro-2,4-dinitrobenzene, ovalbumin