Open Access

Acute and sub-chronic toxicity study of recombinant bovine interferon alpha in rodents

Hai-Yang Yu
Hai-Yang Yu
, 
Dong-Mei Gao
Dong-Mei Gao
, 
Wei Zhou
Wei Zhou
, 
Bing-Bing Xia
Bing-Bing Xia
, 
Zhi-Yuan He
Zhi-Yuan He
, 
Bo Wu
Bo Wu
, 
Min-Zhi Jiang
Min-Zhi Jiang
, 
Ming-Li Wang
Ming-Li Wang
 and 
Jun Zhao
Jun Zhao
   | May 16, 2021
Journal of Veterinary Research's Cover Image
About this article
Previous Article
Next Article
Cite
Published Online: May 16, 2021
Page range: 183 - 192
Received: Sep 17, 2020
Accepted: Apr 09, 2020
DOI: https://doi.org/10.2478/jvetres-2021-0023
Keywords
© 2021 H.Y. Yu et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Effects of rBoIFN-α on feed intake of male (A) and female (B) rats in sub-chronic toxicity test
Effects of rBoIFN-α on feed intake of male (A) and female (B) rats in sub-chronic toxicity test

Fig. 2

Effects of rBoIFN-α on weight gain of male (A) and female (B) rats in sub-chronic toxicity test
Effects of rBoIFN-α on weight gain of male (A) and female (B) rats in sub-chronic toxicity test

Fig. 3

Effects of rBoIFN-α on the morphology of liver, kidney and spleen in Sprague Dawley rats (HE staining, ×200)
Effects of rBoIFN-α on the morphology of liver, kidney and spleen in Sprague Dawley rats (HE staining, ×200)

Effects of rBoIFN-α on blood biochemical indexes in Sprague Dawley rats in the sub-chronic toxicity test

Time Index Gender High-dose group Middle-dose group Low-dose group Control group
Day 31 ALT (U/L) M 51.10 ± 11.03 54.10 ± 7.19 46.80 ± 5.87 51.40 ± 21.00
F 40.80 ± 7.07 43.30 ± 5.08 43.60 ± 7.98 36.00 ± 11.90
AST (U/L) M 194.70 ± 31.81 190.20 ± 54.94 175.70 ± 11.80 197.30 ± 44.16
F 203.70 ± 50.69 182.80 ± 27.34 198.20 ± 29.10 188.20 ± 28.74
BUN (mmol/L) M 3.48 ± 0.34 4.11 ± 0.84 3.56 ± 0.44 3.55 ± 0.72
F 5.00 ± 082 5.00 ± 0.65 5.30 ± 0.33 5.91 ± 1.10
CREAT (μmol/L) M 29.10 ± 3.11 29.70 ± 5.63 27.50 ± 3.36 30.30 ± 1.67
F 32.70 ± 3.42 34.10 ± 4.92 33.20 ± 4.83 37.10 ± 3.77
GLU (mmol/L) M 3.74 ± 1.34 4.02 ± 0.85 4.41 ± 0.30 4.55 ± 1.58
F 2.56 ± 0.77 2.47 ± 0.24 2.90 ± 0.49 2.70 ± 0.44
TP (g/L) M 56.84 ± 1.79 55.60 ± 3.66 56.15 ± 3.89 57.10 ± 1.80
F 61.60 ± 2.33 59.72 ± 4.11 60.70 ± 3.26 60.16 ± 3.54
ALB (g/L) M 42.00 ± 1.07 41.88 ± 2.99 41.58 ± 2.20 41.30 ± 0.48
F 47.01 ± 1.06 45.92 ± 2.80 45.58 ± 2.13 44.44 ± 2.25
CHO (mmol/L) M 2.03 ± 0.23 2.02 ± 0.14 2.11 ± 0.20 1.81 ± 0.35
F 1.88 ± 0.36 2.00 ± 0.10 1.70 ± 0.35 1.70 ± 0.30
TG (mmol/L) M 0.74 ± 0.26 0.81 ± 0.09 1.01 ± 0.14 0.64 ± 0.31
F 0.85 ± 0.29 0.79 ± 0.12 0.69 ± 0.05 0.68 ± 0.19
Day 38 ALT (U/L) M 49.20 ± 2.30 56.20 ± 3.97 65.80 ± 15.98 60.20 ± 15.47
F 38.50 ± 6.43 40.20 ± 5.64 42.00 ± 9.88 42.70 ± 5.17
AST (U/L) M 125.30 ± 13.07 120.20 ± 12.74 153.70 ± 28.60 143.00 ± 18.15
F 124.10 ± 24.77 121.00 ± 17.03 125.50 ± 13.10 132.40 ± 13.85
BUN (mmol/L) M 4.73 ± 0.93 5.01 ± 0.79 4.69 ± 1.06 5.10 ± 0.95
F 6.00 ± 0.32 5.77 ± 0.97 6.48 ± 0.36 5.96 ± 0.66
CREAT (μmol/L) M 29.30 ± 3.91 33.10 ± 6.95 29.00 ± 4.04 31.30 ± 3.91
F 38.70 ± 4.02 38.50 ± 3.65 39.20 ± 3.65 34.90 ± 2.74
GLU (mmol/L) M 6.40 ± 0.71 6.11 ± 0.20 5.32 ± 0.79* 7.10 ± 1.62
F 4.17 ± 0.74 4.70 ± 0.59 5.11 ± 0.51 4.71 ± 0.70
TP (g/L) M 57.24 ± 2.17 58.60 ± 2.46 57.16 ± 0.82 56.86 ± 1.95
F 62.44 ± 2.64 61.30 ± 4.50 63.64 ± 1.34 63.02 ± 1.77
ALB (g/L) M 57.24 ± 2.17 58.50 ± 2.46 57.26 ± 0.82 57.00 ± 1.95
F 31.70 ± 1.55 31.80 ± 2.21 32.20 ± 2.01 32.20 ± 0.79
CHO (mmol/L) M 1.80 ± 0.33 1.91 ± 0.05 1.63 ± 0.14 1.80 ± 0.34
F 1.80 ± 0.18 1.62 ± 0.25 1.63 ± 0.10 1.77 ± 0.20
TG (mmol/L) M 0.90 ± 0.38 1.05 ± 0.52 1.02 ± 0.21 1.18 ± 0.13
F 0.91 ± 0.02 0.86 ± 0.30 0.92 ± 0.06 1.11 ± 0.26

Effects of rBoIFN-α on haematological indexes in Sprague Dawley rats in the sub-chronic toxicity test

Time Index Gender High-dose group Middle-dose group Low-dose group Control group
Day 31 HGB (g/L) M 151.30 ± 4.16 149.00 ± 6.48 147.90 ± 4.64 146.00 ± 0.11
F 144.10 ± 7.98 142.10 ± 8.41 142.90 ± 12.65 147.50 ± 5.37
RBC (× 1012/L) M 7.72 ± 0.16 7.65 ± 0.23 7.78 ± 0.33 7.63 ± 0.13
F 7.63 ± 0.39 7.49 ± 0.35 7.59 ± 0.52 7.64 ± 0.37
WBC (× 109/L) M 14.45 ± 1.29 13.35 ± 2.26* 15.07 ± 2.66 16.23 ± 1.09
F 8.54 ± 1.38 10.79 ± 1.64 8.90 ± 2.31 10.11 ± 1.38
NE (× 109/L) M 1.65 ± 0.29 1.84 ± 0.90 1.86 ± 0.59 2.20 ± 0.42
F 1.18 ± 0.57 1.01 ± 0.25 1.10 ± 0.64 1.41 ± 0.36
BA (× 109/L) M 0.01 ± 0.01 0.00 ± 0.01 0.01 ± 0.01 0.01 ± 0.00
F 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
EO (× 109/L) M 0.10 ± 0.03 0.08 ± 0.03 0.08 ± 0.04 0.10 ± 0.05
F 0.10 ± 0.03 0.10 ± 0.03 0.08 ± 0.04 0.12 ± 0.05
LY (× 109/L) M 12.31 ± 1.08 1.10 ± 2.36 12.66 ± 2.40 13.01 ± 1.10
F 7.02 ± 1.13 9.56 ± 1.44 7.46 ± 1.82 8.30 ± 1.30
MO (× 109/L) M 0.26 ± 0.07 0.23 ± 0.11 0.34 ± 0.11 0.33 ± 0.11
F 0.12 ± 0.08 0.10 ± 0.06 0.14 ± 0.02 0.18 ± 0.05
Day 38 HGB (g/L) M 145.70 ± 8.87 140.50 ± 26.82 149.60 ± 5.02 155.20 ± 8.38
F 134.50 ± 5.35 151.50 ± 9.53 143.70 ± 3.49 142.70 ± 8.29
RBC (× 1012/L) M 7.39 ± 0.50 7.58 ± 0.73 7.68 ± 0.58 8.06 ± 0.34
F 7.00 ± 1.27 7.66 ± 0.46 7.40 ± 0.24 7.50 ± 0.28
WBC (× 109/L) M 10.69 ± 3.91 10.86 ± 1.50 9.01 ± 1.24 11.92 ± 4.08
F 7.42 ± 2.87 10.32 ± 1.24* 8.65 ± 0.92 7.33 ± 2.14
NE (× 109/L) M 0.71 ± 0.15 1.41 ± 0.54 1.05 ± 0.47 1.10 ± 0.33
F 1.00 ± 0.50 1.00 ± 0.21 0.90 ± 0.33 1.00 ± 0.27
BA (× 109/L) M 0.00 ± 0.01 0.00 ± 0.01 0.00 ± 0.00 0.00 ± 0.01
F 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
EO (× 109/L) M 0.05 ± 0.03 0.11 ± 0.09 0.04 ± 0.03 0.10 ± 0.05
F 0.07 ± 0.03 0.11 ± 0.04 0.10 ± 0.15 0.09 ± 0.02
LY (× 109/L) M 9.77 ± 3.84 9.03 ± 1.04 7.65 ± 0.85 10.23 ± 3.69
F 6.09 ± 2.35 8.81 ± 0.83* 7.32 ± 0.90 6.02 ± 2.31
MO (× 109/L) M 0.10 ± 0.05 0.18 ± 0.07 0.10 ± 0.04 0.11 ± 0.06
F 0.10 ± 0.03* 0.16 ± 0.05 0.11 ± 0.04* 0.18 ± 0.07

Effects of rBoIFN-α on parenchymal organ indexes in Sprague Dawley rats in the sub-chronic toxicity test

Time Organ Gender High-dose group Middle-dose group Low-dose group Control group
Day 31 Heart M 3.71 ± 0.28 3.61 ± 0.28 4.02 ± 0.42 3.60 ± 0.22
F 3.71 ± 0.19 3.88 ± 0.57 3.66 ± 0.34 4.01 ± 0.43
Liver M 30.01 ± 3.19 31.53 ± 3.59 31.3 ± 2.28 28.14 ± 2.11
F 33.78 ± 3.10 27.46 ± 4.40 28.19 ± 1.98 32.35 ± 5.27
Spleen M 1.80 ± 0.45 2.01 ± 0.40 2.01 ± 0.21 1.76 ± 0.24
F 2.01 ± 0.19 2.09 ± 0.49 2.27 ± 0.99 1.89 ± 0.23
Lung M 4.72 ± 0.37 5.00 ± 0.54 4.61 ± 0.41 4.30 ± 1.08
F 5.54 ± 0.79 5.01 ± 0.83 5.00 ± 0.24 5.82 ± 0.79
Kidney M 7.67 ± 1.24 7.53 ± 0.64 7.64 ± 0.30 7.53 ± 0.70
F 7.52 ± 0.35 7.50 ± 1.54 6.35 ± 1.96 7.34 ± 0.83
Stomach M 8.74 ± 1.47 8.85 ± 1.64 9.30 ± 1.39 9.30 ± 1.39
F 9.58 ± 3.20 8.44 ± 1.14 7.10 ± 1.32 7.83 ± 1.73
Intestines M 64.34 ± 11.54 64.52 ± 5.79 65.48 ± 16.00 63.52 ± 3.26
F 55.22 ± 12.14 59.91 ± 11.92 58.32 ± 6.33 65.08 ± 9.62
Testis M 11.91 ± 0.70 12.02 ± 0.62 12.26 ± 1.44 12.62 ± 1.00
Uterus and ovaries F 3.72 ± 1.13 4.24 ± 0.73 3.88 ± 0.80 3.14 ± 0.63
Day 38 Heart M 3.45 ± 0.98 3.42 ± 0.21 3.35 ± 0.34 3.55 ± 0.47
F 3.59 ± 0.53 3.60 ± 0.29 3.80 ± 0.58 3.71 ± 0.45
Liver M 33.01 ± 1.98 31.85 ± 2.72 32.61 ± 2.83 32.06 ± 1.23
F 27.84 ± 3.20 29.50 ± 2.41 31.15 ± 2.75 29.31 ± 2.26
Spleen M 1.76 ± 0.17 1.65 ± 0.17 2.01 ± 0.21 2.01 ± 0.43
F 1.83 ± 0.37 2.01 ± 0.10 2.17 ± 0.91 1.80 ± 0.25
Lung M 4.16 ± 0.47 4.42 ± 0.57 4.25 ± 0.36 5.01 ± 1.10
F 4.27 ± 0.29 4.65 ± 0.38 4.41 ± 0.24 4.64 ± 0.44
Kidney M 6.74 ± 0.30 7.04 ± 0.57 6.77 ± 0.17 7.25 ± 0.36
F 6.59 ± 0.88 6.59 ± 0.58 7.03 ± 0.43 6.72 ± 0.64
Stomach M 8.66 ± 1.72 7.78 ± 0.82 8.58 ± 1.45 8.53 ± 0.71
F 8.33 ± 0.86 8.07 ± 0.89 7.49 ± 1.18 7.68 ± 1.74
Intestines M 64.31 ± 8.66 66.15 ± 8.74 66.88 ± 8.01 66.81 ± 7.93
F 61.07 ± 4.78 64.68 ± 11.63 58.82 ± 4.52 66.23 ± 6.21
Testis M 9.59 ± 0.35* 10.33 ± 0.70 9.75 ± 0.43 10.39 ± 0.56
Uterus and ovaries F 3.00 ± 0.74 2.85 ± 0.19 3.32 ± 0.49 3.04 ± 0.85
eISSN:
2450-8608
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
Journal RSS Feed