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Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue

Cassandra Ceccopieri

Cassandra Ceccopieri

Division of Histology and Embryology, Department of Biostructure and Animal Physiology, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Ceccopieri, Cassandra
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Joanna Skonieczna

Joanna Skonieczna

Division of Histology and Embryology, Department of Biostructure and Animal Physiology, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Skonieczna, Joanna
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Jan P. Madej

Jan P. Madej

Division of Histology and Embryology, Department of Biostructure and Animal Physiology, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Madej, Jan P.
  
Jan 29, 2021
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Published Online: Jan 29, 2021
Page range: 125 - 130
Received: Aug 10, 2020
Accepted: Jan 12, 2021
DOI: https://doi.org/10.2478/jvetres-2021-0008
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© 2021 C. Ceccopieri et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – mouflon aorta; black arrowhead – collagen (mainly type I) fibres; white arrowhead – elastic fibres; asterisk – smooth muscle cells C and D – chicken spleen; asterisk – trabeculae; arrowhead – reticular (type III collagen) fibres E and F – rat skin; asterisk – collagen of the dermis. All scale bars =100 μm
Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – mouflon aorta; black arrowhead – collagen (mainly type I) fibres; white arrowhead – elastic fibres; asterisk – smooth muscle cells C and D – chicken spleen; asterisk – trabeculae; arrowhead – reticular (type III collagen) fibres E and F – rat skin; asterisk – collagen of the dermis. All scale bars =100 μm

Fig. 2

Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – pig penis; black arrowhead – basement membrane; asterisk – collagen (mainly type I) fibres; white arrowhead – smooth muscle cells C and D – hyaline cartilage from the trachea of the rabbit E and F – rabbit tooth; asterisk – dentin. All scale bars = 100 μm
Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – pig penis; black arrowhead – basement membrane; asterisk – collagen (mainly type I) fibres; white arrowhead – smooth muscle cells C and D – hyaline cartilage from the trachea of the rabbit E and F – rabbit tooth; asterisk – dentin. All scale bars = 100 μm

Fig. 3

Comparison of different staining methods in the diagnosis of reticulin fibrosis in cavy liverA – haematoxylin-erythrosine saffron method B – Masson–Goldner trichrome C – silver impregnation D – haematoxylin-eosin saffron (HES) method; asterisk – collagen (mainly type I) fibres; arrowhead – reticular (type III collagen) fibres. All scale bars = 100 μm
Comparison of different staining methods in the diagnosis of reticulin fibrosis in cavy liverA – haematoxylin-erythrosine saffron method B – Masson–Goldner trichrome C – silver impregnation D – haematoxylin-eosin saffron (HES) method; asterisk – collagen (mainly type I) fibres; arrowhead – reticular (type III collagen) fibres. All scale bars = 100 μm
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Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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