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Detection of koi herpesvirus (KHV) and carp oedema virus (CEV) in invasive round goby, Neogobius melanostomus Pallas, 1814, from Poland and Germany

Yeonhwa Jin

Yeonhwa Jin

Friedrich-Loeffler-InstitutRiems, Germany
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Jin, Yeonhwa
, 
Natalia Adamkowska

Natalia Adamkowska

Department of Aquatic Bioengineering and Aquaculture West Pomeranian University of Technology in SzczecinSzczecin, Poland
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Adamkowska, Natalia
, 
Jolanta Kiełpińska

Jolanta Kiełpińska

Department of Aquatic Bioengineering and Aquaculture West Pomeranian University of Technology in SzczecinSzczecin, Poland
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Kiełpińska, Jolanta
 and 
Sven Michael Bergmann

Sven Michael Bergmann

Friedrich-Loeffler-InstitutRiems, Germany
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Bergmann, Sven Michael
  
May 27, 2020
Detection of koi herpesvirus (KHV) and carp oedema virus (CEV) in invasive round goby, Neogobius melanostomus Pallas, 1814, from Poland and Germany's Cover Image
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Published Online: May 27, 2020
Page range: 247 - 251
Received: Sep 25, 2019
Accepted: May 14, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0037
Keywords
© 2020 Y. Jin et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Electrophoretic separation of nested PCR products using KHV-Fn/KHV-Rn primers
Electrophoretic separation of nested PCR products using KHV-Fn/KHV-Rn primers

Fig. 2

Alignment of the obtained fragments of the KHV genome with the sequence submitted to GenBank under accession no. MG925491
Alignment of the obtained fragments of the KHV genome with the sequence submitted to GenBank under accession no. MG925491

Primer pairs used in the study to detect the KHV and CEV genomes

PrimerPrimer sequence (5′–3′)Product sizeReferences
KHV-FGACGACGCCGGAGACCTTGTG486 bpGilad et al. (9)
KHV-RCACAAGTTCAGTCTGTTCCTCAAC486 bpGilad et al. (9)
KHV-1FnCTCGCCGAGCAGAGGAAGCGC414 bpBergmann et al. (2)
KHV-1RnTCATGCTCTCCGAGGCCAGCGG414 bpBergmann et al. (2)
KHV-TK-FGGGTTACCTGTACGAG409 bpBercovier et al. (1)
KHV-TK-RCACCCAGTAGATTATGC409 bpBercovier et al. (1)
KHV-TK-FnCGTCGTGAGGAATACGACG348 bpUnpublished: Way in Kempter et al. (15)
KHV-TK-RnACCGTACAGCTCGTACTGG348 bpUnpublished: Way in Kempter et al. (15)
CEVforBATGGAGTATCCAAAGTACTTAG528 bpMatras et al. (18)
CEVrevJCTCTTCACTATTGTGACTT TG528 bpMatras et al. (18)
CEVforBintGTTATCAATGAAATTTGTGTATTG478 bpMatras et al. (18)
CEVrevJintTAGCAAAGTACTACCTCATCC478 bpMatras et al. (18)
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Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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