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Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing

Dulce Anahy Verdugo Escárcega

Dulce Anahy Verdugo Escárcega

Master’s Programme in Sustainable Animal Health and Production, Autonomous University of QueretaroQueretaro, Mexico
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Escárcega, Dulce Anahy Verdugo
, 
Claudia Angélica Perea Razo

Claudia Angélica Perea Razo

Doctorate Programme in Biological Sciences, Autonomous University of QueretaroQueretaro, Mexico
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Razo, Claudia Angélica Perea
, 
Sara González Ruíz

Sara González Ruíz

Doctorate Programme in Biological Sciences, Autonomous University of QueretaroQueretaro, Mexico
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Ruíz, Sara González
, 
Susana Lucia Sosa Gallegos

Susana Lucia Sosa Gallegos

Natural Sciences Department, Autonomous University of QueretaroQueretaro, Mexico
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Gallegos, Susana Lucia Sosa
, 
Feliciano Milián Suazo

Feliciano Milián Suazo

Natural Sciences Department, Autonomous University of QueretaroQueretaro, Mexico
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Suazo, Feliciano Milián
 and 
Germinal Jorge Cantó Alarcón

Germinal Jorge Cantó Alarcón

Natural Sciences Department, Autonomous University of QueretaroQueretaro, Mexico
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Alarcón, Germinal Jorge Cantó
  
Feb 14, 2020
Analysis of bovine tuberculosis transmission in Jalisco, Mexico through whole-genome sequencing's Cover Image
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Published Online: Feb 14, 2020
Page range: 51 - 61
Received: May 10, 2019
Accepted: Feb 03, 2020
DOI: https://doi.org/10.2478/jvetres-2020-0010
Keywords
© 2020 D.A.V. Escárcega et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Bovine tuberculosis, caused by M. bovis, is endemic in Mexico and has had a big impact on public health. Jalisco is considered to be an important dairy region in the country, accounting for approximately 19% of the total milk production. Within Jalisco, the region of Altos Sur holds the largest proportion of the cattle inventory of the state.

Material and Methods

To determine the frequency of bovine tuberculosis in Altos Sur, Jalisco, as well as M. bovis genetic diversity, sampling of tissue (lymph nodes, lungs, and liver) from Holstein cattle was performed in four abattoirs belonging to three municipalities of this region (Tepatitlán de Morelos, San Miguel el Alto, and Arandas). Spoligotyping and whole-genome sequencing were carried out to assess the genetic relationships of M. bovis strains circulating in this area, as well as a comparison to isolates from other places in Mexico.

Results

Prevalence was 15.06%, and distribution similar among the three municipalities. The most frequent spoligotypes were SB0673, SB121, and SB0145. Whole-genome sequencing revealed three main clades (I, II, III), but isolates did not show clustering by region.

Conclusion

Phylogenetic analysis suggested ongoing transmission between herds of the different regions, and no unique source of infection was determined. This hinders efforts under the national program for the control and eradication of the disease, so serious attention must be paid to rural regions such as Altos Sur in order to improve its success.
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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