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Anti-CyHV-3 effect of fluorescent, tricyclic derivative of acyclovir 6-(4-MeOPh)-TACV in vitro

Agnieszka Troszok

Agnieszka Troszok

Institute of Ichthyobiology and AquacultureChybie, Poland
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Troszok, Agnieszka
, 
Ludmiła Kolek

Ludmiła Kolek

Institute of Ichthyobiology and AquacultureChybie, Poland
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Kolek, Ludmiła
, 
Joanna Szczygieł

Joanna Szczygieł

Institute of Ichthyobiology and AquacultureChybie, Poland
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Szczygieł, Joanna
, 
Tomasz Ostrowski

Tomasz Ostrowski

Institute of Bioorganic ChemistryPoznań, Poland
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Ostrowski, Tomasz
, 
Mikołaj Adamek

Mikołaj Adamek

University of Veterinary MedicineHannover, Germany
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Adamek, Mikołaj
 and 
Ilgiz Irnazarow

Ilgiz Irnazarow

Institute of Ichthyobiology and AquacultureChybie, Poland
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Irnazarow, Ilgiz
  
Oct 24, 2019
Anti-CyHV-3 effect of fluorescent, tricyclic derivative of acyclovir 6-(4-MeOPh)-TACV in vitro's Cover Image
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Published Online: Oct 24, 2019
Page range: 513 - 518
Received: Mar 21, 2019
Accepted: Oct 02, 2019
DOI: https://doi.org/10.2478/jvetres-2019-0065
Keywords
© 2019 A. Troszok et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

The tricyclic derivative of acyclovir 3,9-dihydro-3-((2-hydroxyethoxy)methyl)-6-(4-methoxyphenyl)-9-oxo-5H-imidazo (1,2-a)purine T-ACV
The tricyclic derivative of acyclovir 3,9-dihydro-3-((2-hydroxyethoxy)methyl)-6-(4-methoxyphenyl)-9-oxo-5H-imidazo (1,2-a)purine T-ACV

Fig. 2

The cytotoxicity of T-ACV at concentrations of 66.67 and 133.33 μM in CCB and KF1 cell lines at the phase of exponential growth. The graphs depict results yielded by an MTT assay in CCB, n = 9 (A) and KF1, n = 8 (B) and by CV assay in CCB, n =8 (C) and KF1, n = 8 (D). The data are expressed as a percentage of absorbance of the control group (0 μM T-ACV) and presented as mean ± SD. The differences in a paired t-test between the control group and T-ACV group were significant at p ≤ 0.05* or p ≤ 0.01**
The cytotoxicity of T-ACV at concentrations of 66.67 and 133.33 μM in CCB and KF1 cell lines at the phase of exponential growth. The graphs depict results yielded by an MTT assay in CCB, n = 9 (A) and KF1, n = 8 (B) and by CV assay in CCB, n =8 (C) and KF1, n = 8 (D). The data are expressed as a percentage of absorbance of the control group (0 μM T-ACV) and presented as mean ± SD. The differences in a paired t-test between the control group and T-ACV group were significant at p ≤ 0.05* or p ≤ 0.01**

Fig. 3

The anti-CyHV-3 activity of T-ACV in CCB, n = 10 (A) and KF1, n = 8 (B) cell lines determined by plaque assay. The data are expressed as a percentage of plaques of the control group (CyHV-3 infected, 0 μM T-ACV) and presented as mean ± SD. The differences in a paired t-test between the control group and T-ACV group were significant at p ≤ 0.01**
The anti-CyHV-3 activity of T-ACV in CCB, n = 10 (A) and KF1, n = 8 (B) cell lines determined by plaque assay. The data are expressed as a percentage of plaques of the control group (CyHV-3 infected, 0 μM T-ACV) and presented as mean ± SD. The differences in a paired t-test between the control group and T-ACV group were significant at p ≤ 0.01**

Fig. 4

The anti-CyHV-3 activity of T-ACV in CCB, n = 14 (A) and KF1, n = 9 (B) cell lines determined by TaqMan qPCR assay. The data are expressed as a percentage of viral load of the control group (CyHV-3 infected, 0 μM T-ACV) and presented as mean ± SD. The differences in a paired t-test between the control group and T-ACV group were significant at p ≤ 0.01**
The anti-CyHV-3 activity of T-ACV in CCB, n = 14 (A) and KF1, n = 9 (B) cell lines determined by TaqMan qPCR assay. The data are expressed as a percentage of viral load of the control group (CyHV-3 infected, 0 μM T-ACV) and presented as mean ± SD. The differences in a paired t-test between the control group and T-ACV group were significant at p ≤ 0.01**

Primers and probes used for TaqMan qPCR quantification of CyHV-3 DNA copy number

Target Sequence FP Sequence RP Probe Primer sequence source
CyHV-3 KHV-86f KHV-163r KHV-109p FAM-
GACGCCGGAGACCT CGGGTTCTTATTTTT CTTCCTCTGCTCGGCGAGC Gilad et al. (8)
TGTG GTCCTTGTT ACG-BHQ
Carp glucokinase CgGluc-162f CgGluc-230r cgGluc-185p VIC-
ACTGCGAGTGGAGA TCAGGTGTGGAGCG AAGCCAGTGTCAAAATGC Gilad et al. (8)
CACATGAT GACAT TGCCCACT-MGF-NFQ
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Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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