<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Genotype VI of avian avulavirus 1 (AAvV-1) has pigeons and doves as its reservoir and is often termed pigeon paramyxovirus type-1 (PPMV-1). The pathogenesis of PPMV-1 infections in poultry is largely obscure. It is known that PPMV-1 requires a series of passages in chickens before it becomes adapted to gallinaceous poultry.<bold>Material and Methods:</bold> Changes in the genome of PPMV-1 were analysed after serial passages in specific pathogen free (SPF) chickens, using high-throughput sequencing. Additionally, histopathological lesions induced by PPMV-1 in experimentally inoculated pigeons, chickens, and turkeys were evaluated.<bold>Results:</bold> Following six passages of PPMV-1 in chickens, 10 nonsynonymous substitutions were found including one (in the NP protein) which dominated the genetic pool of viral quasispecies. Histopathological changes induced by the post-passage PPMV-1 strain were more prominent than changes wrought by the pre-passaged PPMV-1 strain and the lesions were most intense in pigeons followed by chickens and turkeys.<bold>Conclusion:</bold> PPMV-1 is highly adapted to pigeons and passaging through chickens results in the acquisition of novel amino acids in the polymerase complex, which may alter the pathogenic potential of the virus.</abstract>

Introduction: Genotype VI of avian avulavirus 1 (AAvV-1) has pigeons and doves as its reservoir and is often termed pigeon paramyxovirus type-1 (PPMV-1). The pathogenesis of PPMV-1 infections in poultry is largely obscure. It is known that PPMV-1 requires a series of passages in chickens before it becomes adapted to gallinaceous poultry.Material and Methods: Changes in the genome of PPMV-1 were analysed after serial passages in specific pathogen free (SPF) chickens, using high-throughput sequencing. Additionally, histopathological lesions induced by PPMV-1 in experimentally inoculated pigeons, chickens, and turkeys were evaluated.Results: Following six passages of PPMV-1 in chickens, 10 nonsynonymous substitutions were found including one (in the NP protein) which dominated the genetic pool of viral quasispecies. Histopathological changes induced by the post-passage PPMV-1 strain were more prominent than changes wrought by the pre-passaged PPMV-1 strain and the lesions were most intense in pigeons followed by chickens and turkeys.Conclusion: PPMV-1 is highly adapted to pigeons and passaging through chickens results in the acquisition of novel amino acids in the polymerase complex, which may alter the pathogenic potential of the virus.

Genetic changes in pigeon paramyxovirus type-1 induced by serial passages in chickens and microscopic lesions caused by the virus in various avian hosts

Department of Poultry Diseases, National Veterinary Research Institute

Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Genotype VI of avian avulavirus 1 (AAvV-1) has pigeons and doves as its reservoir and is often termed pigeon paramyxovirus type-1 (PPMV-1). The...

Genetic changes in pigeon paramyxovirus type-1 induced by serial passages in chickens and microscopic lesions caused by the virus in various avian hosts

Published Online: Dec 31, 2018

Page range: 447 - 455

Received: Jun 06, 2018

Accepted: Oct 25, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0059

Keywords
pigeons, poultry, pigeon paramyxovirus type-1, histopathological lesions, high-throughput sequencing

© 2018 Monika Olszewska-Tomczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic changes in pigeon paramyxovirus type-1 induced by serial passages in chickens and microscopic lesions caused by the virus in various avian hosts

Published Online: Dec 31, 2018

Page range: 447 - 455

Received: Jun 06, 2018

Accepted: Oct 25, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0059

Keywordspigeons, poultry, pigeon paramyxovirus type-1, histopathological lesions, high-throughput sequencing

© 2018 Monika Olszewska-Tomczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
pigeons, poultry, pigeon paramyxovirus type-1, histopathological lesions, high-throughput sequencing