<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0030_s_005_w2aab3b7c13b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Inosine pranobex (Methisoprinol, ISO, Isoprinosine) is an immuno-modulatory antiviral drug that has been licensed since 1971 in several countries worldwide. In humans, the drug is approved for the treatment of viral infections, and it might also have therapeutic use in animals. The aims of the presented work were to investigate the genotoxicity of inosine pranobex on BALB/3T3 clone A1 and HepG2 cell lines and to elucidate its mutagenicity using the Ames test.</p></sec><sec id="j_jvetres-2018-0030_s_006_w2aab3b7c13b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>The BALB/3T3 clone A1 and HepG2 cells were incubated with inosine pranobex at concentrations from 0.1 to 1,000 μg/mL. The genotoxicity was determined by comet and micronucleus assays, and the mutagenicity was determined by Ames assay.</p></sec><sec id="j_jvetres-2018-0030_s_007_w2aab3b7c13b1b6b1aab1c16b3Aa"><h3>Results</h3><p>Inosine pranobex did not induce a significant dose-related increase in the number of comets or micronuclei in BALB/3T3 clone A1 and HepG2 cells. Moreover, based on the results of the Ames test, it was concluded that inosine pranobex is not mutagenic in the <italic>Salmonella typhimurium</italic> reverse mutation assay.</p></sec><sec id="j_jvetres-2018-0030_s_008_w2aab3b7c13b1b6b1aab1c16b4Aa"><h3>Conclusion</h3><p>Based on the results of a comet assay, micronucleus assay, and Ames test, it was concluded that inosine pranobex is neither genotoxic nor mutagenic.</p></sec></abstract>

IntroductionInosine pranobex (Methisoprinol, ISO, Isoprinosine) is an immuno-modulatory antiviral drug that has been licensed since 1971 in several countries worldwide. In humans, the drug is approved for the treatment of viral infections, and it might also have therapeutic use in animals. The aims of the presented work were to investigate the genotoxicity of inosine pranobex on BALB/3T3 clone A1 and HepG2 cell lines and to elucidate its mutagenicity using the Ames test.Material and MethodsThe BALB/3T3 clone A1 and HepG2 cells were incubated with inosine pranobex at concentrations from 0.1 to 1,000 μg/mL. The genotoxicity was determined by comet and micronucleus assays, and the mutagenicity was determined by Ames assay.ResultsInosine pranobex did not induce a significant dose-related increase in the number of comets or micronuclei in BALB/3T3 clone A1 and HepG2 cells. Moreover, based on the results of the Ames test, it was concluded that inosine pranobex is not mutagenic in the Salmonella typhimurium reverse mutation assay.ConclusionBased on the results of a comet assay, micronucleus assay, and Ames test, it was concluded that inosine pranobex is neither genotoxic nor mutagenic.

Genotoxicity and mutagenicity of inosine pranobex

The John Paul II Catholic University of Lublin

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine

University of Warmia and Mazury in Olsztyn

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Genotoxicity and mutagenicity of inosine pranobex"}

IntroductionInosine pranobex (Methisoprinol, ISO, Isoprinosine) is an immuno-modulatory antiviral drug that has been licensed since 1971 in several countries...

Genotoxicity and mutagenicity of inosine pranobex

Published Online: Jul 07, 2018

Page range: 207 - 213

Received: Mar 06, 2018

Accepted: Jun 20, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0030

Keywords
Inosine pranobex, Methisoprinol, genotoxicity, mutagenicity

© 2018 Sylwia Tobólska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genotoxicity and mutagenicity of inosine pranobex

Published Online: Jul 07, 2018

Page range: 207 - 213

Received: Mar 06, 2018

Accepted: Jun 20, 2018

DOI: https://doi.org/10.2478/jvetres-2018-0030

KeywordsInosine pranobex, Methisoprinol, genotoxicity, mutagenicity

© 2018 Sylwia Tobólska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Inosine pranobex, Methisoprinol, genotoxicity, mutagenicity