<abstract xmlns="http://www.w3.org/1999/xhtml">Only few studies in the plant tissue culture literature have examined the impact of lanthanoids, or rare earth elements, on <italic>in vitro</italic> plant organogenesis. In this study, using a model plant, hybrid <italic>Cymbidium</italic> Twilight Moon ‘Day Light’, the impact of six lanthanoids (lanthanum (III) nitrate hexahydrate (La(NO3)3 · 6H2O), cerium (III) nitrate hexahydrate (Ce(NO3)3 · 6H2O), neodymium (III) nitrate hexahydrate (Nd(NO3)3 · 6H2O), praseodymium (III) nitrate hexahydrate (Pr(NO3)3 · 6H2O), samarium (III) nitrate hexahydrate (Sm(NO3)3 · 6H2O), gadolinium (III) nitrate hexahydrate (Gd(NO3)3 · 6H2O) on new protocorm-like body <italic>(neo-PLB)</italic> formation on Teixeira <italic>Cymbidium</italic> (TC) medium was examined. 0 (control), 1, 2, 4 and 8 mg·dm-3 of each lanthanoid was tested. All lanthanoids could produce more <italic>neo</italic>-PLBs and <italic>neo-PLB</italic> fresh weight than TC medium lacking plant growth regulators (PGRs), suggesting some PGR-like ability of lanthanoids, although PLB-related traits (percentage of half-PLBs forming <italic>neo</italic>-PLBs; number of <italic>neo</italic>-PLBs formed per half-PLB; fresh weight of half-PLB + <italic>neo</italic>-PLBs) was always significantly lower than TC medium containing PGRs. Except for Gd, all other lanthanoids had no negative impact on the number of new leaves from <italic>neo</italic>-PLB-derived shoots, but all lanthanoids showed a significantly lower plant height, shoot fresh weight and shoot dry weight and, in most cases, SPAD (chlorophyll content) value. In addition, using the same concentration of the six lanthanoids, the ability to fortify root formation of <italic>neo</italic>-PLB-derived plantlets was also assessed. Except for Sm, all other lanthanoids significantly increased the number of roots, root fresh and dry weight.</abstract>

Only few studies in the plant tissue culture literature have examined the impact of lanthanoids, or rare earth elements, on in vitro plant organogenesis. In this study, using a model plant, hybrid Cymbidium Twilight Moon ‘Day Light’, the impact of six lanthanoids (lanthanum (III) nitrate hexahydrate (La(NO3)3 · 6H2O), cerium (III) nitrate hexahydrate (Ce(NO3)3 · 6H2O), neodymium (III) nitrate hexahydrate (Nd(NO3)3 · 6H2O), praseodymium (III) nitrate hexahydrate (Pr(NO3)3 · 6H2O), samarium (III) nitrate hexahydrate (Sm(NO3)3 · 6H2O), gadolinium (III) nitrate hexahydrate (Gd(NO3)3 · 6H2O) on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined. 0 (control), 1, 2, 4 and 8 mg·dm-3 of each lanthanoid was tested. All lanthanoids could produce more neo-PLBs and neo-PLB fresh weight than TC medium lacking plant growth regulators (PGRs), suggesting some PGR-like ability of lanthanoids, although PLB-related traits (percentage of half-PLBs forming neo-PLBs; number of neo-PLBs formed per half-PLB; fresh weight of half-PLB + neo-PLBs) was always significantly lower than TC medium containing PGRs. Except for Gd, all other lanthanoids had no negative impact on the number of new leaves from neo-PLB-derived shoots, but all lanthanoids showed a significantly lower plant height, shoot fresh weight and shoot dry weight and, in most cases, SPAD (chlorophyll content) value. In addition, using the same concentration of the six lanthanoids, the ability to fortify root formation of neo-PLB-derived plantlets was also assessed. Except for Sm, all other lanthanoids significantly increased the number of roots, root fresh and dry weight.

How Do Rare Earth Elements (Lanthanoids) Affect Root Development and Protocorm-Like Body Formation in Hybrid <italic>C<sc>YMBIDIUM?</sc></italic>

Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki cho, Kita Gun, Ikenobe, 761-0795, Japan

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

How Do Rare Earth Elements (Lanthanoids) Affect Root Development and Protocorm-Like Body Formation in Hybrid 

Only few studies in the plant tissue culture literature have examined the impact of lanthanoids, or rare earth elements, on in vitro plant organogenesis. In...

How Do Rare Earth Elements (Lanthanoids) Affect Root Development and Protocorm-Like Body Formation in Hybrid CYMBIDIUM?

Published Online: Sep 10, 2014

Page range: 67 - 75

Received: Jan 09, 2014

Accepted: Mar 20, 2014

DOI: https://doi.org/10.2478/johr-2014-0008

Keywords
lanthanoid, orchid, PLB, rare earth element, root formation, Teixeira (TC) medium

© 2014 Jaime A. Teixeira da Silva

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

How Do Rare Earth Elements (Lanthanoids) Affect Root Development and Protocorm-Like Body Formation in Hybrid CYMBIDIUM?

Published Online: Sep 10, 2014

Page range: 67 - 75

Received: Jan 09, 2014

Accepted: Mar 20, 2014

DOI: https://doi.org/10.2478/johr-2014-0008

Keywordslanthanoid, orchid, PLB, rare earth element, root formation, Teixeira (TC) medium

© 2014 Jaime A. Teixeira da Silva

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
lanthanoid, orchid, PLB, rare earth element, root formation, Teixeira (TC) medium