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Development of Monoclonal Antibodies for Identifying Plant-Parasitic Nematodes

M. Bogale

M. Bogale

  • University of FloridaGainesville,
  • The Ohio State UniversityColumbus,
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Bogale, M.
, 
E. Sampson

E. Sampson

University of FloridaGainesville,
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Sampson, E.
, 
W. Hu

W. Hu

University of FloridaGainesville,
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Hu, W.
, 
A. Baniya

A. Baniya

  • University of FloridaGainesville,
  • University of California-RiversideRiverside,
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Baniya, A.
, 
S. Mishra

S. Mishra

University of FloridaGainesville,
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Mishra, S.
, 
K. Kwon

K. Kwon

University of Wisconsin-MadisonMadison,
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Kwon, K.
, 
H. D. Lopez-Nicora

H. D. Lopez-Nicora

The Ohio State UniversityColumbus,
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Lopez-Nicora, H. D.
 and 
P. DiGennaro

P. DiGennaro

  • University of FloridaGainesville,
  • University of Wisconsin-MadisonMadison,
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DiGennaro, P.
  
Jul 19, 2025
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Article Category: Research Note
Published Online: Jul 19, 2025
Received: Feb 13, 2025
DOI: https://doi.org/10.2478/jofnem-2025-0024
Keywords
© 2025 Bogale, M. et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Indirect ELISA showing the interaction of antigen HG type 0-based monoclonal antibody (mAb) against the four nematode antigen types generated from soybean cyst nematode (SCN) and root-knot nematode (RKN): SCN 1 (HG type 0), SCN 2 (HG type 4.5.7), RKN race 3 and RKN race 4. The concentration of the mAb is in proportion to the template DNA used during the protein synthesis and is therefore in ng/μL.
Indirect ELISA showing the interaction of antigen HG type 0-based monoclonal antibody (mAb) against the four nematode antigen types generated from soybean cyst nematode (SCN) and root-knot nematode (RKN): SCN 1 (HG type 0), SCN 2 (HG type 4.5.7), RKN race 3 and RKN race 4. The concentration of the mAb is in proportion to the template DNA used during the protein synthesis and is therefore in ng/μL.
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Number of monoclonal antibody (mAb) nucleotide sequences generated per antigen type (HG type and race designation for soybean cyst nematode [SCN; Heterodera glycines] and root-knot nematode [RKN; Meloidogyne incognita])_

Antigen type No. V(D) J Spanning Cells Total mAb Sequences
SCN HG type 0 32 3,978
SCN HG type 0 5
SCN HG type 0 3,941
SCN HG type 1.2.3.5.6.7 2 6,183
SCN HG type 1.2.3.5.6.7 2,784
SCN HG type 1.2.3.5.6.7 3,397
RKN race 3 8,995 27,098
RKN race 3 10,178
RKN race 3 7,925
RKN race 4 8,171 21,313
RKN race 4 13,142
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