<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Barley root-knot nematode, <italic>Meloidogyne naasi</italic> <xref ref-type="bibr" rid="j_jofnem-2023-0051_ref_007">Franklin, 1965</xref>, is one of the most important pest nematodes infecting monocots (<xref ref-type="bibr" rid="j_jofnem-2023-0051_ref_007">Franklin, 1965</xref>). Two-inch core soil samples collected from a golf course in Ada County, Idaho were submitted for identification in November of 2019. A high number of <italic>Meloidogyne</italic> sp. juveniles were recovered from both soil samples using sieving and decantation followed by the sugar centrifugal flotation method. They were examined by light microscopy, morphometric measurements, and multiple molecular markers, including the ribosomal 28S D2–D3 and intergenic spacer 2 (IGS-2) regions, mitochondrial markers cytochrome oxidase I (COI) and the interval from COII to 16S, and the protein-coding gene Hsp90. Morphometrics as well as BlastN comparisons with other root-knot nematode sequences from GenBank were consistent with identification as <italic>M. naasi</italic>. Phylogenetic trees inferred from 28S, IGS-2, COI, or Hsp90 alignments each separated the Idaho population into a strongly supported clade with other populations of <italic>M. naasi</italic>, while the COII-16S interval could not resolve <italic>M. naasi</italic> from <italic>M. minor</italic>. This report represents the first morphological and molecular characterization of <italic>Meloidogyne naasi</italic> from turfgrass in Idaho.</p>
</abstract>

Barley root-knot nematode, Meloidogyne naasi Franklin, 1965, is one of the most important pest nematodes infecting monocots (Franklin, 1965). Two-inch core soil samples collected from a golf course in Ada County, Idaho were submitted for identification in November of 2019. A high number of Meloidogyne sp. juveniles were recovered from both soil samples using sieving and decantation followed by the sugar centrifugal flotation method. They were examined by light microscopy, morphometric measurements, and multiple molecular markers, including the ribosomal 28S D2–D3 and intergenic spacer 2 (IGS-2) regions, mitochondrial markers cytochrome oxidase I (COI) and the interval from COII to 16S, and the protein-coding gene Hsp90. Morphometrics as well as BlastN comparisons with other root-knot nematode sequences from GenBank were consistent with identification as M. naasi. Phylogenetic trees inferred from 28S, IGS-2, COI, or Hsp90 alignments each separated the Idaho population into a strongly supported clade with other populations of M. naasi, while the COII-16S interval could not resolve M. naasi from M. minor. This report represents the first morphological and molecular characterization of Meloidogyne naasi from turfgrass in Idaho.

First report of barley root-knot nematode, <italic>Meloidogyne naasi</italic> from turfgrass in Idaho, with multigene molecular characterization

Mycology and Nematology Genetic Diversity and Biology Laboratory

Department of Plant Pathology and Environmental Microbiology

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

First report of barley root-knot nematode,  from turfgrass in Idaho, with multigene molecular characterization

Barley root-knot nematode, Meloidogyne naasi Franklin, 1965, is one of the most important pest nematodes infecting monocots (Franklin, 1965). Two-inch core...

First report of barley root-knot nematode, Meloidogyne naasi from turfgrass in Idaho, with multigene molecular characterization

Article Category: Research Paper

Published Online: Nov 20, 2023

Page range: -

Received: Jun 28, 2023

DOI: https://doi.org/10.2478/jofnem-2023-0051

Keywords
golf course, identification, molecular markers, morphology, taxonomy

© 2023 Andrea M. Skantar et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

First report of barley root-knot nematode, Meloidogyne naasi from turfgrass in Idaho, with multigene molecular characterization

Article Category: Research Paper

Published Online: Nov 20, 2023

Page range: -

Received: Jun 28, 2023

DOI: https://doi.org/10.2478/jofnem-2023-0051

Keywordsgolf course, identification, molecular markers, morphology, taxonomy

© 2023 Andrea M. Skantar et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
golf course, identification, molecular markers, morphology, taxonomy