<abstract xmlns="http://www.w3.org/1999/xhtml">The nematicidal activity of radish bulb (<italic>Raphanus sativus</italic>) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot nematode <italic>Meloidogyne incognita</italic> model. The EC50 of RME after 3 d of J2 immersion in test solutions was 312 ± 65 μg/ml. However, no activity was noted for RAE (EC50 > 1,000 μg/ml). The chemical composition analysis of the methanol extract carried out by the GC–MS technique showed that 4-methylthio-3-butenyl isothiocyanate (raphasatin) was the most abundant compound at 20%. This pure compound strongly induced J2 paralysis with an EC50 of 1.3 ± 0.2 μg/ml after 24 hr. Comparison with other 11 selected isothiocyanates with structure similarity showed that the double bond at position 3 of the alkyl side chain is important for nematicidal activity, followed by the sulfur group at position 5 and the benzyl group at position 2. In addition, raphasatin showed the highest nematicidal activity with the corresponding lowest antioxidant activity of about 92 ± 18 μg/ml. In conclusion, the results of this investigation reveal that <italic>R. sativus</italic> and its major compound raphasatin can be integrated into the pest management system.</abstract>

The nematicidal activity of radish bulb (Raphanus sativus) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot nematode Meloidogyne incognita model. The EC50 of RME after 3 d of J2 immersion in test solutions was 312 ± 65 μg/ml. However, no activity was noted for RAE (EC50 > 1,000 μg/ml). The chemical composition analysis of the methanol extract carried out by the GC–MS technique showed that 4-methylthio-3-butenyl isothiocyanate (raphasatin) was the most abundant compound at 20%. This pure compound strongly induced J2 paralysis with an EC50 of 1.3 ± 0.2 μg/ml after 24 hr. Comparison with other 11 selected isothiocyanates with structure similarity showed that the double bond at position 3 of the alkyl side chain is important for nematicidal activity, followed by the sulfur group at position 5 and the benzyl group at position 2. In addition, raphasatin showed the highest nematicidal activity with the corresponding lowest antioxidant activity of about 92 ± 18 μg/ml. In conclusion, the results of this investigation reveal that R. sativus and its major compound raphasatin can be integrated into the pest management system.

Nematicidal effect of Raphasatin from <italic>Raphanus sativus</italic> against <italic>Meloidogyne incognita</italic>

Journal of Nematology

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Nematicidal effect of Raphasatin from  against 

The nematicidal activity of radish bulb (Raphanus sativus) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot...

Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita

Article Category: Research Paper

Published Online: Nov 17, 2022

Page range: -

Received: May 31, 2022

DOI: https://doi.org/10.2478/jofnem-2022-0050

Keywords
DPPH, isothiocyanates, raphasatin

© 2022 Aissani and Sebai. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita

Article Category: Research Paper

Published Online: Nov 17, 2022

Page range: -

Received: May 31, 2022

DOI: https://doi.org/10.2478/jofnem-2022-0050

KeywordsDPPH, isothiocyanates, raphasatin

© 2022 Aissani and Sebai. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
DPPH, isothiocyanates, raphasatin