Surveillance of Viruses in Varroa destructor Samples Collected from Honey Bee Colonies in Ontario, Canada, between 2015 and 2019

Viral copy numbers and number of positive honey bee samples and V_ destructor samples (n=50) base on qPCR

Accession number (NCBI)	Description	Number of honey bee positive samples	Number of V. destructor positive samples	Mean viral copy numbers (±SEM) in honey bees	Mean viral copy numbers (±SEM) in V. destructor
AY053371.1	ABPV	0	10	Not detected	2.41×109±0.0
MT482476.1	BQCV	14	1	4.41×105±4.24×105	6.15×106±0.0
MN114562.1	CBPV	1	0	1.39×101	Not detected
MT096529.1	DWV-A	36	38	6.79×109±5.49×109	1.44×1010±5.57×109
MG599488.1	IAPV	1	1	5.71×103	5.71×103
MT096516.1	KBV	1	1	1.80×102	1.79×102
MT482477.1	SBV	7	7	1.89×105±1.74×105	1.89×105±0.00

Taxon identification number (NCBI), description, number of positive V_ destructor samples (from a total of 50 samples tested), and average reads in the clade based on Kraken v2 outputs (confidence score of 0_65)

Taxon identification number (NCBI)	Description	Number of positive samples	Average reads from positive samples (Kraken v2)
198112	DWV-A	18	84.89±87.68
232800	VDV-1	9	444.44±0.00
2056384	VDV-2	13	6.96±0.81
2056385	VDV-3	12	16.92±0.00
1682186	Bee macula virus	10	176.90±0.00
1100043	Apis mellifera filamentous virus	1	309
92395	BQCV	1	3
92444	ABPV	3	32.67±0.00

Presence of viruses and viral levels (number of RNA sequences or transcripts) of 35 out of the 50 V_ destructor mites used in the study and analyzed with Kraken (Wood & Salzberg, 2014)

Sample ID	Year of sampling	198112 (DWV-A)	232800 (VDV-1/DWV-B)	2056384 (VDV-2)	2056385 (VDV-3)	1682186 (Bee Macula like virus)	1100043 (Apis mellifera filamentous virus)	92395 (BQCV)	92444 (ABPV)
15-054309-0010	2015	1	0	6	0	7	0	0	0
15-054393-0010	2015	0	0	3	25	59	0	0	0
15-054972-0010	2015	0	0	7	0	0	309	0	0
15-055033-0010	2015	0	0	3	49	0	0	3	0
15-055622-0010	2015	22	0	12	57	534	0	0	0
15-055628-0010	2015	0	0	1	31	0	0	0	0
15-055681-0010	2015	0	0	8	7	0	0	0	0
15-057660-0010	2015	744	0	3	13	0	0	0	0
15-057733-0010	2015	0	0	4	0	0	0	0	0
15-059145-0010	2015	0	0	4	0	0	0	0	0
16-072563-0010	2016	0	0	2	0	0	0	0	0
16-073231-0014	2016	212	0	0	0	546	0	0	0
16-076218-0011	2016	310	0	0	10	447	0	0	0
16-079403-0012	2016	82	0	0	2	94	0	0	0
16-079404-0011	2016	0	0	1	0	0	0	0	0
17-071828-0003	2017	0	0	0	3	0	0	0	0
17-073654-0001	2017	0	0	0	2	0	0	0	0
17-074745-0002	2017	6	752	0	0	0	0	0	0
17-077871-0001	2017	62	16	0	1	47	0	0	60
17-080092-0002	2017	16	19	2	0	2	0	0	0
17-080430-0001	2017	0	0	0	0	0	0	0	0
17-080445-0001	2017	1	604	0	0	0	0	0	0
17-083166-0001	2017	0	1	0	0	0	0	0	0
18-073335-0002	2018	0	0	0	0	0	0	0	1
18-075350-0001	2018	1	0	0	0	0	0	0	0
18-075359-0001	2018	2	2236	0	0	0	0	0	0
18-076045-0004	2018	21	0	0	0	23	0	0	37
18-077007-0001	2018	16	0	0	0	10	0	0	0
18-079834-0001	2018	0	366	0	0	0	0	0	0
18-081107-0001	2018	26	0	0	0	0	0	0	0
18-084972-0002	2018	0	2	0	0	0	0	0	0
19-073791-0001	2019	1	0	0	0	0	0	0	0
19-076087-0002	2019	0	4	1	0	0	0	0	0
19-077315-0001	2019	4	1	0	3	0	0	0	0
19-081189-0005	2019	1	0	0	0	0	0	0	0