Surveillance of Viruses in Varroa destructor Samples Collected from Honey Bee Colonies in Ontario, Canada, between 2015 and 2019
, , , , , , , , and | Aug 18, 2022
About this article
Article Category: Short communication
Published Online: Aug 18, 2022
Page range: 209 - 215
Received: Jan 26, 2022
Accepted: Jun 13, 2022
DOI: https://doi.org/10.2478/jas-2022-0009
Keywords
© 2022 Nuria Morfin et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Viral copy numbers and number of positive honey bee samples and V. destructor samples (n=50) base on qPCR
| AY053371.1 | ABPV | 0 | 10 | Not detected | 2.41×109±0.0 |
| MT482476.1 | BQCV | 14 | 1 | 4.41×105±4.24×105 | 6.15×106±0.0 |
| MN114562.1 | CBPV | 1 | 0 | 1.39×101 | Not detected |
| MT096529.1 | DWV-A | 36 | 38 | 6.79×109±5.49×109 | 1.44×1010±5.57×109 |
| MG599488.1 | IAPV | 1 | 1 | 5.71×103 | 5.71×103 |
| MT096516.1 | KBV | 1 | 1 | 1.80×102 | 1.79×102 |
| MT482477.1 | SBV | 7 | 7 | 1.89×105±1.74×105 | 1.89×105±0.00 |
Taxon identification number (NCBI), description, number of positive V. destructor samples (from a total of 50 samples tested), and average reads in the clade based on Kraken v2 outputs (confidence score of 0.65)
| 198112 | DWV-A | 18 | 84.89±87.68 |
| 232800 | VDV-1 | 9 | 444.44±0.00 |
| 2056384 | VDV-2 | 13 | 6.96±0.81 |
| 2056385 | VDV-3 | 12 | 16.92±0.00 |
| 1682186 | 10 | 176.90±0.00 | |
| 1100043 | 1 | 309 | |
| 92395 | BQCV | 1 | 3 |
| 92444 | ABPV | 3 | 32.67±0.00 |
Presence of viruses and viral levels (number of RNA sequences or transcripts) of 35 out of the 50 V. destructor mites used in the study and analyzed with Kraken (Wood & Salzberg, 2014)
| 15-054309-0010 | 2015 | 1 | 0 | 6 | 0 | 7 | 0 | 0 | 0 |
| 15-054393-0010 | 2015 | 0 | 0 | 3 | 25 | 59 | 0 | 0 | 0 |
| 15-054972-0010 | 2015 | 0 | 0 | 7 | 0 | 0 | 309 | 0 | 0 |
| 15-055033-0010 | 2015 | 0 | 0 | 3 | 49 | 0 | 0 | 3 | 0 |
| 15-055622-0010 | 2015 | 22 | 0 | 12 | 57 | 534 | 0 | 0 | 0 |
| 15-055628-0010 | 2015 | 0 | 0 | 1 | 31 | 0 | 0 | 0 | 0 |
| 15-055681-0010 | 2015 | 0 | 0 | 8 | 7 | 0 | 0 | 0 | 0 |
| 15-057660-0010 | 2015 | 744 | 0 | 3 | 13 | 0 | 0 | 0 | 0 |
| 15-057733-0010 | 2015 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 |
| 15-059145-0010 | 2015 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 |
| 16-072563-0010 | 2016 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| 16-073231-0014 | 2016 | 212 | 0 | 0 | 0 | 546 | 0 | 0 | 0 |
| 16-076218-0011 | 2016 | 310 | 0 | 0 | 10 | 447 | 0 | 0 | 0 |
| 16-079403-0012 | 2016 | 82 | 0 | 0 | 2 | 94 | 0 | 0 | 0 |
| 16-079404-0011 | 2016 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| 17-071828-0003 | 2017 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
| 17-073654-0001 | 2017 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| 17-074745-0002 | 2017 | 6 | 752 | 0 | 0 | 0 | 0 | 0 | 0 |
| 17-077871-0001 | 2017 | 62 | 16 | 0 | 1 | 47 | 0 | 0 | 60 |
| 17-080092-0002 | 2017 | 16 | 19 | 2 | 0 | 2 | 0 | 0 | 0 |
| 17-080430-0001 | 2017 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 17-080445-0001 | 2017 | 1 | 604 | 0 | 0 | 0 | 0 | 0 | 0 |
| 17-083166-0001 | 2017 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-073335-0002 | 2018 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| 18-075350-0001 | 2018 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-075359-0001 | 2018 | 2 | 2236 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-076045-0004 | 2018 | 21 | 0 | 0 | 0 | 23 | 0 | 0 | 37 |
| 18-077007-0001 | 2018 | 16 | 0 | 0 | 0 | 10 | 0 | 0 | 0 |
| 18-079834-0001 | 2018 | 0 | 366 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-081107-0001 | 2018 | 26 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-084972-0002 | 2018 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| 19-073791-0001 | 2019 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 19-076087-0002 | 2019 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
| 19-077315-0001 | 2019 | 4 | 1 | 0 | 3 | 0 | 0 | 0 | 0 |
| 19-081189-0005 | 2019 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |