Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of  and Their Toxicity Against

Fiorella G. De Piano; Matias D. Maggi; Facundo R. Meroi Arceitto; Marcela C. Audisio; Martín Eguaras; Sergio R. Ruffinengo

Open Access

Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor

Fiorella G. De Piano

Matias D. Maggi

Facundo R. Meroi Arceitto

Marcela C. Audisio

Martín Eguaras

and

Sergio R. Ruffinengo

| Jul 02, 2020

Journal of Apicultural Science

Volume 64 (2020): Issue 1 (June 2020)

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Published Online: Jul 02, 2020

Page range: 55 - 66

Received: Apr 04, 2019

Accepted: Feb 11, 2020

DOI: https://doi.org/10.2478/jas-2020-0009

Keywords
bacterial metabolites, beneficial bacteria, nutrition,

© 2020 Fiorella G. De Piano et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cumulative survival of bees fed on different concentrations of CFS 1 (cell-free supernatant produced by Lactobacillus johnsonii AJ5) for six days.

Cumulative survival of bees fed on different concentrations of CFS 2 (cell-free supernatant produced by Enterococcus faecium SM21) for six days.

Cumulative survival of bees fed on different concentrations of CFS 3 (cell-free supernatant produced by Bacillus subtilis subsp. subtilis Mori2) for six days.

Mean and standard deviation of soluble protein per bee (mg/mg) for each CFS concentration

	CFS concentration	Mean ± SD
Control	Syrup	0.0105^bc ± 0.0022
	MRS/syrup	0.0098^c ± 0.0029
	BHI/syrup	0.0106^bc ± 0.0018

CFS1	1 % v/v CFS 1/syrup	0.0106^bc ± 0.0023
	6 % v/v CFS 1/syrup	0.0112^abc ± 0.0027
	20 % v/v CFS 1/syrup	0.0119^abc ± 0.0033
	40 % v/v CFS 1/syrup	0.0146^a± 0.0054

CFS2	1 % v/v CFS 2/syrup	0.0115^abc ± 0.0029
	5 % v/v CFS 2/syrup	0.0115^abc ± 0.0036
	15 % v/v CFS 2/syrup	0.0125^abc ± 0.0025
	30 % v/v CFS 2/syrup	0.0136^ab ± 0.0049

CFS3	5 % v/v CFS 3/syrup	0.0110^abc ± 0.0026
	15 % v/v CFS 3/syrup	0.0127^abc ± 0.0048
	30 % v/v CFS 3/syrup	0.0142^a± 0.0039
	60 % v/v CFS 3/syrup	-

Mean and standard deviation of dead mite at 72 h in contact with bees feeding with different CFSs concentrations

	CFS concentration	Dead mite ± SD	Effectiveness (%)
Control	syrup	0.125^a ± 0.337	12.5
	MRS/syrup	0.130^a ± 0.344	13
	BHI/syrup	0.133^a ± 0.345	13.3

CFS1	1 % v/v CFS 1/syrup	0.218^a ± 0.420	21.8
	6 % v/v CFS 1/syrup	0.419^ab ± 0.501	41.9
	20 % v/v CFS 1/syrup	0.333^ab ± 0.479	33.3
	40 % v/v CFS 1/syrup	0.566^b ± 0.504	56.6

CFS2	1 % v/v CFS 2/syrup	0.130^a ± 0.344	13
	5 % v/v CFS 2/syrup	0.214^a ± 0.417	21.4
	15 % v/v CFS 2/syrup	0.200^a ± 0.406	20
	30 % v/v CFS 2/syrup	0.166^a ± 0.379	16.6

CFS3	5 % v/v CFS 3/syrup	0.259^a ± 0.446	25.9
	15 % v/v CFS 3/syrup	0.233^a ± 0.430	23.3
	30 % v/v CFS 3/syrup	0.241^a ± 0.435	24.1
	60 % v/v CFS 3/syrup	0.240^a ± 0.435	24

Mean and standard deviation of fat body mass per bee (mg/bee) for each CFS concentration

	CFS concentration	Mean ± SD
Control	Syrup	0.0021^ab ± 0.0012
	MRS/syrup	0.0018^ab ± 0.0012
	BHI/syrup	0.0018^ab ± 0.0009

CFS1	1 % v/v CFS 1/syrup	0.0019^ab ± 0.0010
	6 % v/v CFS 1/syrup	0.0018^ab ± 0.0010
	20 % v/v CFS 1/syrup	0.0019^ab ± 0.0009
	40 % v/v CFS 1/syrup	0.0020^ab ± 0.0010

CFS2	1 % v/v CFS 2/syrup	0.0019^ab ± 0.0013
	5 % v/v CFS 2/syrup	0.0021^ab ± 0.0017
	15 % v/v CFS 2/syrup	0.0015^b ± 0.0008
	30 % v/v CFS 2/syrup	0.0022^a± 0.0013

CFS3	5 % v/v CFS 3/syrup	0.0018^ab ± 0.0010
	15 % v/v CFS 3/syrup	0.0023^a ± 0.0011
	30 % v/v CFS 3/syrup	0.0019^ab ± 0.0008
	60 % v/v CFS 3/syrup	-

V. destructor mortality (%) at different CFS concentrations (% v/v) at 7 h of contact exposition

	CFS concentration	Mortality %
Control	Water	0
	MRS/water	0
	BHI/water	0

CFS1	10 % v/v CFS1/water	3.33
	30 % v/v CFS1/water	11.1
	60 % v/v CFS1/water	3.57
	100 % v/v CFS1/water	6.66

CFS2	10 % v/v CFS2/water	0
	30 % v/v CFS2/water	0
	60 % v/v CFS2/water	0
	100 % v/v CFS2/water	3.33

CFS3	10 % v/v CFS3/water	0
	30 % v/v CFS3/water	0
	60 % v/v CFS3/water	0
	100 % v/v CFS3/water	0

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Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor

Article Category: Original Article

Published Online: Jul 02, 2020

Page range: 55 - 66

Received: Apr 04, 2019

Accepted: Feb 11, 2020

DOI: https://doi.org/10.2478/jas-2020-0009

Keywords
bacterial metabolites, beneficial bacteria, nutrition,

© 2020 Fiorella G. De Piano et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Mean and standard deviation of soluble protein per bee (mg/mg) for each CFS concentration

Mean and standard deviation of dead mite at 72 h in contact with bees feeding with different CFSs concentrations

Mean and standard deviation of fat body mass per bee (mg/bee) for each CFS concentration

V. destructor mortality (%) at different CFS concentrations (% v/v) at 7 h of contact exposition

Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor

Article Category: Original Article

Published Online: Jul 02, 2020

Page range: 55 - 66

Received: Apr 04, 2019

Accepted: Feb 11, 2020

DOI: https://doi.org/10.2478/jas-2020-0009

Keywordsbacterial metabolites, beneficial bacteria, nutrition,

© 2020 Fiorella G. De Piano et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Mean and standard deviation of soluble protein per bee (mg/mg) for each CFS concentration

Mean and standard deviation of dead mite at 72 h in contact with bees feeding with different CFSs concentrations

Mean and standard deviation of fat body mass per bee (mg/bee) for each CFS concentration

V. destructor mortality (%) at different CFS concentrations (% v/v) at 7 h of contact exposition

Keywords
bacterial metabolites, beneficial bacteria, nutrition,