<abstract xmlns="http://www.w3.org/1999/xhtml">The study was conducted to determine the antioxidant and anti-diabetic properties of bee pollen. The phenol content of extracts was 1.43, 2.04, 2.10 and 1.79 mg gallic acid g-1, respectively. Total flavonoid content was 0.78, 1.39, 0.86 and 0.79 mg gallic acid g-1 respectively. Bee pollen extracts have the potential to scavenge free radicals and lower blood glucose due to the presence of phenols and flavonoids. The aqueous-ethanol extract had the lowest IC50 for HRS and TAC, 0.53mg/mL and 0.25mg/mL respectively, which depicts that this extract alleviates the destructive effect of hydroxyl radicals. Methanol extract had the lowest IC50 0.21 mg/mL) for DPPH inhibition, hence it was able to scavenge the DPPH radicals. Ethanol extract had the lowest IC50 (0.04 mg/mL) for 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid inhibition, hence it can protect living cells from ABTS cations. The aqueous-ethanol extract displayed higher inhibition of α-amylase (4.51 mg/mL) while the aqueous extract exhibited a higher inhibition of α-glucosidase (0.60 mg/mL), which slows down the breakdown of disaccharides and reduces sugar buildup in the bloodstream. Bee pollen has potential antioxidative and antidiabetic activity.</abstract>

The study was conducted to determine the antioxidant and anti-diabetic properties of bee pollen. The phenol content of extracts was 1.43, 2.04, 2.10 and 1.79 mg gallic acid g-1, respectively. Total flavonoid content was 0.78, 1.39, 0.86 and 0.79 mg gallic acid g-1 respectively. Bee pollen extracts have the potential to scavenge free radicals and lower blood glucose due to the presence of phenols and flavonoids. The aqueous-ethanol extract had the lowest IC50 for HRS and TAC, 0.53mg/mL and 0.25mg/mL respectively, which depicts that this extract alleviates the destructive effect of hydroxyl radicals. Methanol extract had the lowest IC50 0.21 mg/mL) for DPPH inhibition, hence it was able to scavenge the DPPH radicals. Ethanol extract had the lowest IC50 (0.04 mg/mL) for 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid inhibition, hence it can protect living cells from ABTS cations. The aqueous-ethanol extract displayed higher inhibition of α-amylase (4.51 mg/mL) while the aqueous extract exhibited a higher inhibition of α-glucosidase (0.60 mg/mL), which slows down the breakdown of disaccharides and reduces sugar buildup in the bloodstream. Bee pollen has potential antioxidative and antidiabetic activity.

Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes <italic>In Vitro</italic> Assessment

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes  Assessment

The study was conducted to determine the antioxidant and anti-diabetic properties of bee pollen. The phenol content of extracts was 1.43, 2.04, 2.10 and 1.79...

Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment

Published Online: Dec 26, 2019

Page range: 315 - 325

Received: Feb 07, 2019

Accepted: Jul 11, 2019

DOI: https://doi.org/10.2478/jas-2019-0020

Keywords
alpha-glucosidase, amylase, antidiabetic, antioxidant, bee-pollen

© 2019 Oluremi M. Daudu, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment

Published Online: Dec 26, 2019

Page range: 315 - 325

Received: Feb 07, 2019

Accepted: Jul 11, 2019

DOI: https://doi.org/10.2478/jas-2019-0020

Keywordsalpha-glucosidase, amylase, antidiabetic, antioxidant, bee-pollen

© 2019 Oluremi M. Daudu, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
alpha-glucosidase, amylase, antidiabetic, antioxidant, bee-pollen