<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Chlorpromazine (CPZ) is still a commonly prescribed antipsychotic which causes poorly understood idiosyncratic toxicity such as cholestasis, phospholipidosis and steatosis. CPZ has diverse cellular targets and exerts various toxicity mechanisms whose exploration is necessary to understand CPZ side effects. We report here that CPZ causes a decrease of total lipid content in <italic>Saccharomyces cerevisiae</italic> at the same dose range as that used on mammalian cells. The observed lipid decrease was obvious after 4 and 9 hours of treatment, and disappeared after 24 hours due to cells adaptation to the chemical stress. The inhibitory effect of CPZ was antagonized by the antioxidant N-acetyl L-cysteine and is likely caused by the parent compound. The obtained results demonstrate that yeast model is valid to investigate the involved CPZ toxicity mechanisms, particularly in terms of lipids alteration. This would contribute to understand CPZ side effects in simple model and reduce experimentation on animals.</p></abstract>

Chlorpromazine (CPZ) is still a commonly prescribed antipsychotic which causes poorly understood idiosyncratic toxicity such as cholestasis, phospholipidosis and steatosis. CPZ has diverse cellular targets and exerts various toxicity mechanisms whose exploration is necessary to understand CPZ side effects. We report here that CPZ causes a decrease of total lipid content in Saccharomyces cerevisiae at the same dose range as that used on mammalian cells. The observed lipid decrease was obvious after 4 and 9 hours of treatment, and disappeared after 24 hours due to cells adaptation to the chemical stress. The inhibitory effect of CPZ was antagonized by the antioxidant N-acetyl L-cysteine and is likely caused by the parent compound. The obtained results demonstrate that yeast model is valid to investigate the involved CPZ toxicity mechanisms, particularly in terms of lipids alteration. This would contribute to understand CPZ side effects in simple model and reduce experimentation on animals.

Short communication: Chlorpromazine causes a time-dependent decrease of lipids in <italic>Saccharomyces cerevisiae</italic>

Laboratory of Experimental and Clinical Pharmacology, Faculty of Sciences

Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Short communication: Chlorpromazine causes a time-dependent decrease of lipids in 

Chlorpromazine (CPZ) is still a commonly prescribed antipsychotic which causes poorly understood idiosyncratic toxicity such as cholestasis, phospholipidosis...

Short communication: Chlorpromazine causes a time-dependent decrease of lipids in Saccharomyces cerevisiae

Published Online: Feb 20, 2020

Page range: 41 - 44

Received: May 19, 2019

Accepted: Jul 11, 2019

DOI: https://doi.org/10.2478/intox-2019-0006

Keywords
phenothiazine, chlorpromazine toxicity, lipids, oxidative stress

© 2019 Dina Muhieddine et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Short communication: Chlorpromazine causes a time-dependent decrease of lipids in Saccharomyces cerevisiae

Published Online: Feb 20, 2020

Page range: 41 - 44

Received: May 19, 2019

Accepted: Jul 11, 2019

DOI: https://doi.org/10.2478/intox-2019-0006

Keywordsphenothiazine, chlorpromazine toxicity, lipids, oxidative stress

© 2019 Dina Muhieddine et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
phenothiazine, chlorpromazine toxicity, lipids, oxidative stress