<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Biofilms on food contact materials represent public health issues because they are resistant to cleaning and disinfection. This study aims to assess the <italic>Bacillus cereus</italic> biofilm formation capacity on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials. The biofilm biomass was analysed with the crystal violet assay method. We used the standard strain <italic>B. cereus</italic> CCM 2010, wild strain <italic>B. cereus</italic> 100 and spores of those two strains. The results show that both the vegetative form the bacteria and it spores form large amounts of biofilm on silicone, followed by polyethylene terephthalate, Teflon, and aluminium. More detailed analysis has shown that spores form more biomass on all materials in comparison to the vegetative form and that the standard strains form low levels of biofilm in contrast to the wild strains. Selecting proper material with the lowest biofilm formation potential can prevent or reduce food contamination and consequently increase food safety.</p></abstract>

Biofilms on food contact materials represent public health issues because they are resistant to cleaning and disinfection. This study aims to assess the Bacillus cereus biofilm formation capacity on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials. The biofilm biomass was analysed with the crystal violet assay method. We used the standard strain B. cereus CCM 2010, wild strain B. cereus 100 and spores of those two strains. The results show that both the vegetative form the bacteria and it spores form large amounts of biofilm on silicone, followed by polyethylene terephthalate, Teflon, and aluminium. More detailed analysis has shown that spores form more biomass on all materials in comparison to the vegetative form and that the standard strains form low levels of biofilm in contrast to the wild strains. Selecting proper material with the lowest biofilm formation potential can prevent or reduce food contamination and consequently increase food safety.

Biofilm formation capacity of <italic>Bacillus cereus</italic> on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials

University of Ljubljana Faculty of Health Sciences

Sanitarno inženirstvo International Journal of Sanitary Engineering Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Biofilm formation capacity of  on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials

Biofilms on food contact materials represent public health issues because they are resistant to cleaning and disinfection. This study aims to assess the...

Biofilm formation capacity of Bacillus cereus on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials

Published Online: Sep 08, 2020

Page range: 4 - 11

Received: Jun 26, 2019

Accepted: Dec 30, 2019

DOI: https://doi.org/10.2478/ijser-2019-0002

Keywords
biofilm, food contact materials

© 2020 Martina Oder et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Biofilm formation capacity of Bacillus cereus on silicone, polyethylene terephthalate, Teflon, and aluminium food contact materials

Published Online: Sep 08, 2020

Page range: 4 - 11

Received: Jun 26, 2019

Accepted: Dec 30, 2019

DOI: https://doi.org/10.2478/ijser-2019-0002

Keywordsbiofilm, food contact materials

© 2020 Martina Oder et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
biofilm, food contact materials