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Molecular and morphological characterization of the root -lesion nematode, Pratylenchus neglectus, on corn from Henan Province of China


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Fig. 1

Light micrographs of Pratylenchus neglectus from corn in Henan Province of China. Females (A -M): A and M, Entire body; B: Anterior region; C-E: lip region; F: Anterior end of genital gland; G: Lateral line; H: Post-vulval region and ovary; I-L: tail region. Scale bars: 50μm (A, M) and 20μm (B-L).
Light micrographs of Pratylenchus neglectus from corn in Henan Province of China. Females (A -M): A and M, Entire body; B: Anterior region; C-E: lip region; F: Anterior end of genital gland; G: Lateral line; H: Post-vulval region and ovary; I-L: tail region. Scale bars: 50μm (A, M) and 20μm (B-L).

Fig. 2

PCR amplification of the rDNA-ITS and rDNA 28S D2-D3 expansion region of Pratylenchus neglectus. M: DL2000 Marker; 1: rDNA-ITS; 2: D2-D3 expansion region of the 28S rDNA.
PCR amplification of the rDNA-ITS and rDNA 28S D2-D3 expansion region of Pratylenchus neglectus. M: DL2000 Marker; 1: rDNA-ITS; 2: D2-D3 expansion region of the 28S rDNA.

Fig. 3

Bayesian tree of Pratylenchus as inferred from rDNA-ITS sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.
Bayesian tree of Pratylenchus as inferred from rDNA-ITS sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.

Fig. 4

Bayesian tree of Pratylenchus as inferred from rDNA 28S D2-D3 sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.
Bayesian tree of Pratylenchus as inferred from rDNA 28S D2-D3 sequences under GTR+I+G model. Posterior probabilities more than 50% are given for appropriate clades. Newly obtained sequence is indicated in bold font.

Measurements of the Pratylenchus neglectus population morphological characteristics.

Character SY-X Pourjam et al. (1999) Ryss (1988)
n 16 32 10
L 459.0 ± 23.0(431.0 – 498.0) 490(300 – 590) 600(410 – 700)
a 22.9 ± 1.6(20.0 – 26.1) 26.7(22.3 – 32.2) 24(17 – 31)
b 5.7 ± 0.5(5.0 – 6.6) 6.0(4.3 – 7.6) 6.3(4.9 – 7.1)
b’ 4.3 ± 0.3(3.9 – 4.7) 4.3(3.4 – 5.2) __
c 18.7 ± 2.2(15.2 – 24.7) 21.4(14.8 – 28.2) 21(13 – 31)
c’ 2.1 ± 0.3(1.5 – 2.6) 2.1(1.5 – 3.0) 1.9(1.5 – 2.5)
V 80.5 ± 2.1(77.5 – 84.6) 82(80 – 85) 83(77 – 85)
Stylet length 15.8 ± 1.1(14.3 – 17.4) 16(14 – 18) 16(15.5 – 17.5)
Stylet shaft 8.1 ± 0.5(7.3 – 9.3) __ __
Stylet knob width 4.1 ± 0.4(3.2 – 4.8) __ __
Stylet knob height 2.0 ± 0.3(1.6 – 2.7) __ __
DGO from stylet base 2.5 ± 0.5(1.4 – 3.3) __ __
center of metacorpus 45.7 ± 2.7(40.3 – 50.5) __ __
end of pharyngeal gland lobe 107.0 ± 4.3(98.5 – 113.0) __ __
Anterior end to excretory pore 74.5 ± 4.5(68.5 – 85.0) __ __
Pharyngeal overlap 26.7 ± 4.9 (19.6 – 39.4) __ __
Max body diam. 20.1 ± 1.6 (17.8 – 23.7) __ __
Vulval body diam. 17.9 ± 1.2(16.0 – 20.00) __ __
Anal body diam. 12.0 ± 1.1(10.6 – 14.8) __ __
Anterior genital trace length 147.4 ± 21.9(102.8 – 192.7) __ __
Tail length 24.8 ± 2.4(20.0 – 29.0) __ __
No. of tail annuli 20.7 ± 2.3(18 – 24) __ __
Vulva to annus distance 68.0 ± 11.5(53.5 – 96.5) __ __
Post- uterine sac length 19.7 ± 4.2(13.0 – 21.7) __ __
Lateral field width 7.2 ± 1.1(5.8 – 8.8) __ __
Lip width 8.1 ± 0.4(7.1 – 8.6) __ __
Lip height 2.4 ± 0.4(1.7 – 3.1) __ __
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