<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Root-lesion nematodes, <italic>Pratylenchus</italic> spp., are economically important pathogens because of their detrimental and economic impact on a wide range of crops. In August 2018, two samples of both roots and rhizosphere soil were collected from a corn field in Liangyuanqu of Shangqiu city, Henan Province, China. Root-lesion nematodes were recovered from the roots and soil samples using the modified Baermann funnel extraction method. Both the morphological characters and molecular analysis of the internal transcribed spacer (ITS) and D2-D3 expansion region of 28S ribosomal RNA sequences confirmed that the root-lesion nematode population collected from corn in this study was <italic>P. neglectus</italic>. Phylogenetic analyses showed that this isolate formed a highly supported clade with other <italic>P. neglectus</italic> isolates. To the best of our knowledge, this is the first report of <italic>P. neglectus</italic> on corn in Henan Province of China. This study reports the first partial sequences of 28S D2-D3 region of <italic>P. neglectus</italic> on corn in China. Due to the great harmfulness of root-lesion nematodes to corn, care should be taken to prevent the spread of <italic>P. neglectus</italic> to other regions in China. At the same time, further study on the biological characteristics of <italic>P. neglectus</italic> is needed, which will be helpful to develop corresponding management and control strategies.</p></abstract>

Root-lesion nematodes, Pratylenchus spp., are economically important pathogens because of their detrimental and economic impact on a wide range of crops. In August 2018, two samples of both roots and rhizosphere soil were collected from a corn field in Liangyuanqu of Shangqiu city, Henan Province, China. Root-lesion nematodes were recovered from the roots and soil samples using the modified Baermann funnel extraction method. Both the morphological characters and molecular analysis of the internal transcribed spacer (ITS) and D2-D3 expansion region of 28S ribosomal RNA sequences confirmed that the root-lesion nematode population collected from corn in this study was P. neglectus. Phylogenetic analyses showed that this isolate formed a highly supported clade with other P. neglectus isolates. To the best of our knowledge, this is the first report of P. neglectus on corn in Henan Province of China. This study reports the first partial sequences of 28S D2-D3 region of P. neglectus on corn in China. Due to the great harmfulness of root-lesion nematodes to corn, care should be taken to prevent the spread of P. neglectus to other regions in China. At the same time, further study on the biological characteristics of P. neglectus is needed, which will be helpful to develop corresponding management and control strategies.

Molecular and morphological characterization of the root -lesion nematode, <italic>Pratylenchus neglectus</italic>, on corn from Henan Province of China

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Molecular and morphological characterization of the root -lesion nematode, , on corn from Henan Province of China

Root-lesion nematodes, Pratylenchus spp., are economically important pathogens because of their detrimental and economic impact on a wide range of crops. In...

Molecular and morphological characterization of the root -lesion nematode, Pratylenchus neglectus, on corn from Henan Province of China

Published Online: Dec 25, 2021

Page range: 385 - 393

Received: May 01, 2021

Accepted: Oct 29, 2021

DOI: https://doi.org/10.2478/helm-2021-0044

Keywords
Corn (), Root-lesion nematodes, Identification, Phylogeny,

© 2021 Y. H. Xia, Y. K. Liu, P. H. Hao, H. X. Yuan, K. Wang, H. L. Li, Y. Li, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Molecular and morphological characterization of the root -lesion nematode, Pratylenchus neglectus, on corn from Henan Province of China

Published Online: Dec 25, 2021

Page range: 385 - 393

Received: May 01, 2021

Accepted: Oct 29, 2021

DOI: https://doi.org/10.2478/helm-2021-0044

KeywordsCorn (), Root-lesion nematodes, Identification, Phylogeny,

© 2021 Y. H. Xia, Y. K. Liu, P. H. Hao, H. X. Yuan, K. Wang, H. L. Li, Y. Li, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Corn (), Root-lesion nematodes, Identification, Phylogeny,