<abstract xmlns="http://www.w3.org/1999/xhtml">The effect of probiotic strains (<italic>Enterococcus faecium</italic> EF55, <italic>E. faecium</italic> CCM7420, <italic>E. faecium</italic> CCM8558, <italic>E. durans</italic> ED26E/7, <italic>Lactobacillus fermentum</italic> CCM7421, <italic>L. plantarum</italic> 17L/1) on the production of superoxide anion (O2-) in peritoneal macrophages of <italic>Trichinella spiralis</italic> infected mice was examined. <italic>E. faecium</italic> EF55 and <italic>E. faecium</italic> CCM8558 strains increased the O2-production prior to parasitic infection,at the day7of application.A significant inhibition of the O2- production caused by <italic>T. spiralis</italic> infection on day 5 <italic>post</italic> infection (p.i.) was prevented by all examined strains. Lactobacilli stimulated metabolic activity of macrophages during intestinal and early muscular phase (from day 5 to 25 p.i.) of trichinellosis. Enterococci increased the O2- production in early intestinal phase (day 5 p.i.) and during the muscular phase of trichinellosis (days 25 and 32 p.i.). Respected increase in macrophage’s metabolic activity induced by probiotic treatment in the intestinal phase of trichinellosis augmented the host antiparasite defence (damage and killing of newborn larvae with reactive oxygen species from macrophages).</abstract>

The effect of probiotic strains (Enterococcus faecium EF55, E. faecium CCM7420, E. faecium CCM8558, E. durans ED26E/7, Lactobacillus fermentum CCM7421, L. plantarum 17L/1) on the production of superoxide anion (O2-) in peritoneal macrophages of Trichinella spiralis infected mice was examined. E. faecium EF55 and E. faecium CCM8558 strains increased the O2-production prior to parasitic infection,at the day7of application.A significant inhibition of the O2- production caused by T. spiralis infection on day 5 post infection (p.i.) was prevented by all examined strains. Lactobacilli stimulated metabolic activity of macrophages during intestinal and early muscular phase (from day 5 to 25 p.i.) of trichinellosis. Enterococci increased the O2- production in early intestinal phase (day 5 p.i.) and during the muscular phase of trichinellosis (days 25 and 32 p.i.). Respected increase in macrophage’s metabolic activity induced by probiotic treatment in the intestinal phase of trichinellosis augmented the host antiparasite defence (damage and killing of newborn larvae with reactive oxygen species from macrophages).

Probiotic bacteria can modulate murine macrophage’s superoxide production in <italic>Trichinella spiralis</italic> infection

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Probiotic bacteria can modulate murine macrophage’s superoxide production in  infection

The effect of probiotic strains (Enterococcus faecium EF55, E. faecium CCM7420, E. faecium CCM8558, E. durans ED26E/7, Lactobacillus fermentum CCM7421, L....

Intestinal phase (numbers of adults)

			Days post infection

	5		11		18
	(mean ± S.D. )	reduction (%)	(mean ± S.D.)	reduction (%)	(mean ± S.D.)	reduction (%)
T.spiralis (control)	295 ± 24		229 ± 37		2±2
E.faecium EF55 + T.spiralis	293 ± 38	0.7	160 ±39	30.1	0±0	100
E.faecium CCM8558 + T.spiralis	244 ± 25	17.3	*107 ±25	53.3	0±0	100
E.faecium CCM7420 + T.spiralis	235 ± 35	20.3	*112±14	51.1	0±0	100
E.durans ED26E/7 + T.spiralis	256 ±7	13.2	*142 ±29	38	1±2	50
L.fermentum CCM7421 + T.spiralis	209 ± 21	29.2	210 ±27	8.3	40 ±12	0
L.plantarum 17L/L1 + T.spiralis	326 ± 48	0	192 ±8	16.2	1±2	0

Probiotic bacteria can modulate murine macrophage’s superoxide production in Trichinella spiralis infection

Article Category: Research Article

Published Online: Aug 05, 2020

Page range: 226 - 234

Received: Nov 12, 2019

Accepted: Mar 17, 2020

DOI: https://doi.org/10.2478/helm-2020-0024

Keywords
probiotic strains, mice, macrophages, superoxide anion

© 2020 M. Vargová, Z. Hurníková, V. Revajová, A. Lauková, E. Dvorožňáková, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

Fig. 2

Parasite burden in mice treated with probiotic bacteria and infected with T. spiralis.

Probiotic bacteria can modulate murine macrophage’s superoxide production in Trichinella spiralis infection

Article Category: Research Article

Published Online: Aug 05, 2020

Page range: 226 - 234

Received: Nov 12, 2019

Accepted: Mar 17, 2020

DOI: https://doi.org/10.2478/helm-2020-0024

Keywordsprobiotic strains, mice, macrophages, superoxide anion

© 2020 M. Vargová, Z. Hurníková, V. Revajová, A. Lauková, E. Dvorožňáková, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

Fig. 2

Parasite burden in mice treated with probiotic bacteria and infected with T. spiralis.

Keywords
probiotic strains, mice, macrophages, superoxide anion