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Toward the Analysis of Lymphocyte Development in Space: PCR-Based Amplification of T-Cell Receptor Excision Circles (TRECs) Aboard the International Space Station

Elizabeth Reizis

Elizabeth Reizis

Stuyvesant High SchoolNew York, USA
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Reizis, Elizabeth
, 
Diana Cai

Diana Cai

  • Biological and Biomedical Sciences, Harvard Medical SchoolBoston, USA
  • Discovery Sciences, Janssen R&DCambridge, USA
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Cai, Diana
, 
Lee Serpas

Lee Serpas

Department of Pathology, New York University Grossman School of MedicineNew York, USA
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Serpas, Lee
, 
Emily J. Gleason

Emily J. Gleason

Cambridge, USA
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Gleason, Emily J.
, 
Kathryn Martin

Kathryn Martin

Cambridge, USA
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Martin, Kathryn
, 
Kevin D. Foley

Kevin D. Foley

Houston, USA
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Foley, Kevin D.
, 
D. Scott Copeland

D. Scott Copeland

Houston, USA
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Copeland, D. Scott
, 
Sebastian Kraves

Sebastian Kraves

Cambridge, USA
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Kraves, Sebastian
 and 
Ezequiel Alvarez Saavedra

Ezequiel Alvarez Saavedra

Cambridge, USA
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Saavedra, Ezequiel Alvarez
  
Dec 31, 2021
Toward the Analysis of Lymphocyte Development in Space: PCR-Based Amplification of T-Cell Receptor Excision Circles (TRECs) Aboard the International Space Station's Cover Image
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Article Category: Research Note
Published Online: Dec 31, 2021
Page range: 159 - 163
DOI: https://doi.org/10.2478/gsr-2021-0012
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© 2021 Elizabeth Reizis et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

TREC formation and primer design. Schematic of TREC formation following VJ recombination at the Tcra locus. Primers are depicted by arrows and are designed to face toward each other following formation of the TRECs (Hazenberg et al., 2001). PCR amplification is expected when primers face each other.
TREC formation and primer design. Schematic of TREC formation following VJ recombination at the Tcra locus. Primers are depicted by arrows and are designed to face toward each other following formation of the TRECs (Hazenberg et al., 2001). PCR amplification is expected when primers face each other.

Figure 2

TRECs detected across a range of ages in mice. (A) Agarose gel electrophoresis of TRECs from the thymus of 2-month-old wildtype mice amplified by PCR in flight and on the ground. Numbers indicate starting concentration of purified DNA in nanograms. (B) Agarose gel electrophoresis of PCR-amplified TRECs from peripheral blood obtained from mice over a range of ages. Rag KO = Rag knockout mice. Thymic DNA = DNA isolated from thymus of 2-month-old wildtype mice. In each reaction, the starting concentration of purified DNA was 10 nanograms.
TRECs detected across a range of ages in mice. (A) Agarose gel electrophoresis of TRECs from the thymus of 2-month-old wildtype mice amplified by PCR in flight and on the ground. Numbers indicate starting concentration of purified DNA in nanograms. (B) Agarose gel electrophoresis of PCR-amplified TRECs from peripheral blood obtained from mice over a range of ages. Rag KO = Rag knockout mice. Thymic DNA = DNA isolated from thymus of 2-month-old wildtype mice. In each reaction, the starting concentration of purified DNA was 10 nanograms.
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