<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The NASA GeneLab Data System (GLDS) was recently developed to facilitate cross-experiment comparisons in order to understand the response of microorganisms to the human spaceflight environment. However, prior spaceflight experiments have been conducted using a wide variety of different hardware, media, culture conditions, and procedures. Such confounding factors could potentially mask true differences in gene expression between spaceflight and ground control samples. In an attempt to mitigate such confounding factors, we describe here the development of a standardized set of hardware, media, and protocols for liquid cultivation of microbes in Biological Research in Canisters (BRIC) spaceflight hardware, using the model bacteria <italic>Bacillus subtilis</italic> strain 168 and <italic>Staphylococcus aureus</italic> strain UAMS-1 as examples.</p></abstract>

The NASA GeneLab Data System (GLDS) was recently developed to facilitate cross-experiment comparisons in order to understand the response of microorganisms to the human spaceflight environment. However, prior spaceflight experiments have been conducted using a wide variety of different hardware, media, culture conditions, and procedures. Such confounding factors could potentially mask true differences in gene expression between spaceflight and ground control samples. In an attempt to mitigate such confounding factors, we describe here the development of a standardized set of hardware, media, and protocols for liquid cultivation of microbes in Biological Research in Canisters (BRIC) spaceflight hardware, using the model bacteria Bacillus subtilis strain 168 and Staphylococcus aureus strain UAMS-1 as examples.

Establishing Standard Protocols for Bacterial Culture in Biological Research in Canisters (BRIC) Hardware

Department of Microbiology and Cell Science

Gravitational and Space Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The NASA GeneLab Data System (GLDS) was recently developed to facilitate cross-experiment comparisons in order to understand the response of microorganisms to...

	SVT		FL		GC
Actual or Simulated Activity	Day	Date	Day	Date	Day	Date
Integration/Handover	0	03/29	0	07/15	0	09/13
Launch	8	04/06	3	07/18	3	09/16
Docking	11	04/09	5	07/20	5	09/18
Unpacking/stowage	12	04/10	6	07/21	6	09/19
Actuation	14	04/12	7	07/22	7	09/20
Transfer to −80°C (B. subtilis)	15	04/13	8	07/23	8	09/21
Transfer to −80°C (S. aureus)	16	04/14	9	07/24	9	09/22
Undocking and splashdown			42	07/26
Arrival at KSC			46	07/30
Deintegration	20	04/18	54	09/07	21	10/04

Establishing Standard Protocols for Bacterial Culture in Biological Research in Canisters (BRIC) Hardware

Article Category: Research Article

Published Online: Jul 18, 2020

Page range: 58 - 69

DOI: https://doi.org/10.2478/gsr-2016-0013

Keywords
Bacteria, Biological Research in Canisters, BRIC, GeneLab, International Space Station, ISS, Spaceflight, Spores,

© 2020 Patricia Fajardo-Cavazos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Schedule of activities for BRIC-23 Science Verification Test (SVT), Flight (FL), and Ground Control (GC) experiments*.

Establishing Standard Protocols for Bacterial Culture in Biological Research in Canisters (BRIC) Hardware

Article Category: Research Article

Published Online: Jul 18, 2020

Page range: 58 - 69

DOI: https://doi.org/10.2478/gsr-2016-0013

KeywordsBacteria, Biological Research in Canisters, BRIC, GeneLab, International Space Station, ISS, Spaceflight, Spores,

© 2020 Patricia Fajardo-Cavazos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Schedule of activities for BRIC-23 Science Verification Test (SVT), Flight (FL), and Ground Control (GC) experiments*.

Keywords
Bacteria, Biological Research in Canisters, BRIC, GeneLab, International Space Station, ISS, Spaceflight, Spores,