<abstract xmlns="http://www.w3.org/1999/xhtml">
<p>Cleft lip (CL), palate (CP), or both (CLP) are one of the most common congenital abnormalities in humans, causing a heavy burden to the affected and their next of kin. We examined biopsy material from CL of seven children: Six 2 to 6 month-old babies and one 4-year-old boy. The samples were taken at the first surgical cleft lip repair. Light microscopy (LM) haematoxylin and eosin stained paraffin sections and toluidine blue stained 0.5–1 μm Durcupan sections from material processed for transmission electron microscopy (TEM), revealed abnormal “ragged” wavy muscle fibres in all seven children. The routine TEM confirmed our results LM; we found no other changes in the soft tissues in TEM; blood vessels of loose connective tissue and nerve fibres were normal. Therefore, we believe that myopathic changes in the cleft lip muscle fibres cannot be of neuronal origin. </p>
</abstract>

Cleft lip (CL), palate (CP), or both (CLP) are one of the most common congenital abnormalities in humans, causing a heavy burden to the affected and their next of kin. We examined biopsy material from CL of seven children: Six 2 to 6 month-old babies and one 4-year-old boy. The samples were taken at the first surgical cleft lip repair. Light microscopy (LM) haematoxylin and eosin stained paraffin sections and toluidine blue stained 0.5–1 μm Durcupan sections from material processed for transmission electron microscopy (TEM), revealed abnormal “ragged” wavy muscle fibres in all seven children. The routine TEM confirmed our results LM; we found no other changes in the soft tissues in TEM; blood vessels of loose connective tissue and nerve fibres were normal. Therefore, we believe that myopathic changes in the cleft lip muscle fibres cannot be of neuronal origin.

Structure of Soft Tissues in Congenital Unilateral Cleft Lip, Light and Electron Microscopic Observations

University of Veterinary Medicine and Pharmacy in Košice

Medical Faculty of the University of P. J. Šafárik

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cleft lip (CL), palate (CP), or both (CLP) are one of the most common congenital abnormalities in humans, causing a heavy burden to the affected and their next...

Structure of Soft Tissues in Congenital Unilateral Cleft Lip, Light and Electron Microscopic Observations

Published Online: Mar 22, 2024

Page range: 62 - 73

Received: Dec 12, 2023

Accepted: Feb 28, 2024

DOI: https://doi.org/10.2478/fv-2024-0008

Keywords
congenital cleft lip, biopsy, light and electron microscopy, myopathy

© 2024 Martin Zibrín et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Structure of Soft Tissues in Congenital Unilateral Cleft Lip, Light and Electron Microscopic Observations

Published Online: Mar 22, 2024

Page range: 62 - 73

Received: Dec 12, 2023

Accepted: Feb 28, 2024

DOI: https://doi.org/10.2478/fv-2024-0008

Keywordscongenital cleft lip, biopsy, light and electron microscopy, myopathy

© 2024 Martin Zibrín et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
congenital cleft lip, biopsy, light and electron microscopy, myopathy