<abstract xmlns="http://www.w3.org/1999/xhtml">
Thiacloprid is an insecticide of the neonicotinoid family that acts on the insect’s nAChR receptor and causes its paralysis and subsequent death. The genotoxicity of the insecticide Calypso® 480 SC (with the active substance thiacloprid) to human peripheral lymphocytes was examined <italic>in vitro</italic> by comet analysis and phosphorylated H2AX, where we detected DNA breaks. To detect DNA damage in comet analysis, we used thiacloprid-based insecticide in three different concentrations (60, 240, and 480 μg.ml−1) during 2 h of exposure. We recorded a statistically significant percentage of damage at a concentration of 240 and 480 μg.ml−1. We monitored the toxicity of thiacloprid using yH2AX foci, and we did not observe a statistically significant number of foci compared to the negative control.
</abstract>

Thiacloprid is an insecticide of the neonicotinoid family that acts on the insect’s nAChR receptor and causes its paralysis and subsequent death. The genotoxicity of the insecticide Calypso® 480 SC (with the active substance thiacloprid) to human peripheral lymphocytes was examined in vitro by comet analysis and phosphorylated H2AX, where we detected DNA breaks. To detect DNA damage in comet analysis, we used thiacloprid-based insecticide in three different concentrations (60, 240, and 480 μg.ml−1) during 2 h of exposure. We recorded a statistically significant percentage of damage at a concentration of 240 and 480 μg.ml−1. We monitored the toxicity of thiacloprid using yH2AX foci, and we did not observe a statistically significant number of foci compared to the negative control.

DNA Damage Detection After Pesticide Exposure

University of Veterinary Medicine and Pharmacy in Kosice

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"DNA Damage Detection After Pesticide Exposure"}

Thiacloprid is an insecticide of the neonicotinoid family that acts on the insect’s nAChR receptor and causes its paralysis and subsequent death. The...

DNA Damage Detection After Pesticide Exposure

Published Online: Mar 22, 2024

Page range: 31 - 36

Received: Dec 08, 2023

Accepted: Feb 14, 2024

DOI: https://doi.org/10.2478/fv-2024-0004

Keywords
comet assay, DNA damage, neonicotinoids, thiacloprid, H2AX

© 2024 Sabína Baloďanská et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

DNA Damage Detection After Pesticide Exposure

Published Online: Mar 22, 2024

Page range: 31 - 36

Received: Dec 08, 2023

Accepted: Feb 14, 2024

DOI: https://doi.org/10.2478/fv-2024-0004

Keywordscomet assay, DNA damage, neonicotinoids, thiacloprid, H2AX

© 2024 Sabína Baloďanská et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
comet assay, DNA damage, neonicotinoids, thiacloprid, H2AX