1. bookVolume 64 (2020): Issue 1 (March 2020)
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Reversal of Diabetic Complications in Andrology Parameters of Alloxan-Induced Diabetic Male Wistar Rats Treated with Cleome rutidosperma Leaves

Published Online: 13 Apr 2020
Volume & Issue: Volume 64 (2020) - Issue 1 (March 2020)
Page range: 19 - 26
Received: 22 Mar 2019
Accepted: 10 Dec 2019
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

Male infertility is one of the complications associated with diabetes mellitus. This study reported the effect of managing experimentally-induced diabetes in male Wistar rats with Cleome rutidosperma leaf extract at doses of 100 mg.kg−1, 200 mg.kg−1 or 400 mg.kg−1 for 14 days. Further deterioration or amelioration of reproductive derangement was assessed by changes in sperm morphology, sperm characteristics, and testicular histo-pathology. Andrology profile of diabetic untreated rats showed significant (P < 0.05) derangements compared to normoglycaemic rats. The profile of diabetic rats treated with the extract of C. rutidosperma, especially at the 200 mg.kg−1 dose showed a significant decrease in abnormal sperm cells, increases in sperm motility, live/ dead ratio and count compared to the untreated diabetic rats. Histopathology showed spermatogenic arrest and degeneration of Sertoli cells in the diabetic untreated rats, but was restored in the C. rutidosperma treated rats. This study showed evidence of infertility associated with diabetes and the ameliorative ability of C. rutidosperma on infertility demonstrated by improved andrological parameters. Traditional management of diabetes with C. rutidosperma leaves should therefore be encouraged in both humans and other animals, especially males with reproductive intentions.

Keywords

1. Adesuyi, A. O., Elumm, I. K., Adaramola, F. B., Nwokocha, A. F. M., 2012: Nutritional and phytochemical screening of Garcinia kola. Adv. J. Food Sci. Tech., 4, 1, 9—14.Search in Google Scholar

2. Afshari, M., Malayeri, A. R., Mohammadshahi, M., 2019: Effects of Eryngium caucasicum extract on testosterone, inflammation and oxidative status of nicotinamide-streptozotocin induced type-2 diabetes in male rats. J. Contemp. Med. Sci., 5, 2, 77—81. Available at <http://www.jocms.org/index.php/jcms/article/view/568>. Date accessed: July 15, 2019.Search in Google Scholar

3. Agarwal, A., Prabakaran, S. A., Said, T. M., 2005: Prevention of oxidative stress injury to sperm. J. Androl., 26, 6, 654—660. DOI: 10.2164/jandrol.05016.10.2164/jandrol.05016Search in Google Scholar

4. Alves, M. G., Martins, A. D., Cavaco, J. E., Socorro, S., Oliveira, P. F., 2013: Diabetes, insulin-mediated glucose metabolism and Sertoli/blood-testis barrier function. Tissue Barriers, 1, 2: e23992. DOI: 10.4161/tisb.23992.10.4161/tisb.23992Search in Google Scholar

5. Bahmani, M., Zargaran, A., Rafieian-Kopaeic, M., Saki, K., 2014: Ethnobotanical study of medicinal plants used in the management of diabetes mellitus in the Urmia, Northwest Iran. Asian Pac. J. Trop. Med., 7, 1, S348—354. DOI:10.1016/S1995-7645(14)60257-1.10.1016/S1995-7645(14)60257-1Search in Google Scholar

6. Ballester, J., Muñoz, M. C., Domínguez, J., Rigau, T., Guinovart, J. J., Rodríguez-Gil, J. E., 2004: Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. J. Androl., 25, 5, 706—719. DOI: 10.1002/j.1939-4640.2004.tb02845.x.10.1002/j.1939-4640.2004.tb02845.x15292100Search in Google Scholar

7. Bancroft, J. D., Gamble, M., 2008:Theory and Practice of Histology Techniques. 6th edn., Churchill Livingstone Elsevier, Philadelphia, 83—134.Search in Google Scholar

8. Banihani, S. A., 2018: Ginger and testosterone. Biomolecules, 8, 4, 119. DOI: 10.3390/biom8040119.10.3390/biom8040119631609330360442Search in Google Scholar

9. Bansal, M., Kaushal, N., 2014:Oxidative Stress Mechanisms and their Modulation. Springer New Delhi, India. 1—167. DOI: 10.1007/978-81-322-2032-9.10.1007/978-81-322-2032-9Search in Google Scholar

10. Bose, A., Mondal, S., Gupta, J. K., Ghosh, T., Debbhuti, D., Si, S., 2008: Antioxidant and free radical scavenging activities of Cleome rutidosperma. Oriental Pharm. Exp. Med., 8, 2, 135—145. DOI 10.3742/OPEM.2008.8.2.135.10.3742/OPEM.2008.8.2.135Search in Google Scholar

11. Bose, A., Saravanan, V. S., Karunanidhi, N., Gupta, J. K., 2004: Analgesic and locomotor activity of extracts of Cleome rutidosperma DC. Indian J. Pharm. Sci., 66, 795—797.Search in Google Scholar

12. Cooper, T. G., Noonan, E., Von Eckardstein, S., Auger, J., Baker, H. W., Behre, H. M., et al., 2010: World Health Organization reference values for human semen characteristics. Hum. Reprod. Update, 16, 3, 231—245. DOI:10.1093/humupd/dmp048.10.1093/humupd/dmp04819934213Search in Google Scholar

13. Ding, G. L., Liu, Y., Liu, M. E., Pan, J. X., Guo, M. X., Sheng, J. Z., Huang, H. F., 2015: The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis. Asian J. Androl., 17, 6, 948—953. DOI: 10.4103/1008-682X.150844.10.4103/1008-682X.150844481495325814158Search in Google Scholar

14. Folasire, O. F., Oridupa, O. A., Owolabi, A. J., Adepoju, O. T., 2016: Anti-hyperglycemic effect of cocoyam (Xanthosoma sagittifollium) corm in alloxan-induced diabetic albino rats. Int. J. Nutr. Metabol., 8, 4, 24—29. DOI: 10.5897/IJNAM2016.0200.10.5897/IJNAM2016.0200Search in Google Scholar

15. Han, X. X., Jiang, Y. P., Liu, N., Jing, Wu, J., Yang, J. M., Li, Y. X., et al., 2019: Protective effects of Astragalin on spermatogenesis in streptozotocin-induced diabetes in male mice by improving antioxidant activity and inhibiting inflammation. Biomed. Pharmacother., 110, 561—570. DOI: 10.1016/j. biopha.2018.12.012.Search in Google Scholar

16. Kadir, M. F., Bin Sayeed, M. S., Shams, T., Mia, M. M. K., 2012: Ethnobotanical survey of medicinal plants used by Bangladeshi traditional health practitioners in the management of diabetes mellitus. J. Ethnopharmacol., 144, 3, 605—611. DOI: 10.1016/j.jep.2012.09.050.10.1016/j.jep.2012.09.05023063956Search in Google Scholar

17. Kamaruzaman, K. A., Aizat, W. M., Noor, M. M., 2018: Gynura procumbens improved fertility of diabetic rats: Preliminary study of sperm proteomic. Evid-Based Compl. Alt. Med., Vol. 2018, Article ID 9201539, 13 pp. DOI: 10.1155/2018/9201539.10.1155/2018/9201539618634330363997Search in Google Scholar

18. Khaki, A., Fathiazad, F., Nouri, M., 2013: Effects of watermelon seed extract (Citrullus vulgaris) on spermatogenesis in rat. Int. J. Women’s Health Reprod. Sci., 1, 99—104.10.15296/ijwhr.2013.16Search in Google Scholar

19. La Vignera, S., Condorelli, R., Vicari, E., D’Agata, R., Calogero, A. E., Amaral, S., et al., 2012:Diabetes mellitus and sperm parameters. J. Androl., 33, 2, 145—153. DOI: 10.2164/jandrol.111.013193.10.2164/jandrol.111.01319321474785Search in Google Scholar

20. Lata, N., Dubey, V., 2010: Preliminary phytochemical screening of Eichhornia crassipes: the world’s worst aquatic weed. J. Pharm. Res., 3, 6,1240—1242.Search in Google Scholar

21. Loeken, M. R., 2012: A new role for pancreatic insulin in the male reproductive axis. Diabetes, 61, 1667—1668. DOI: 10.2337/db12-0539.10.2337/db12-0539337965722723275Search in Google Scholar

22. Mallidis, C., Agbaje, I., McClure, N., Kliesch, S., 2011: The influence of diabetes mellitus on male reproductive function: a poorly investigated aspect of male infertility. Urologe A, 50, 33—37. DOI: 10.1007/s00120-010-2440-3.10.1007/s00120-010-2440-321207007Search in Google Scholar

23. Mazaheri, M., Shahdadi, V., Nazari, B. A., 2014: Molecular and biochemical effect of alcoholic extract of Alpinia galanga on rat spermatogenesis process. Iranian J. Reprod. Med., 11, 765—70.Search in Google Scholar

24. Muralidhara, S. B., 2007: Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: Its progression and genotoxic consequences. Reprod. Toxicol., 23, 4, 578—587. DOI: 10.1016/j.reprotox.2007.02.001.10.1016/j.reprotox.2007.02.00117360155Search in Google Scholar

25. Olayemi, F. O., 2010: A review on some causes of male infertility. Afr. J. Biotech., 9, 20, 2834—2842.Search in Google Scholar

26. Onwueme, I. C., Sinha, T. D., 1991:Feed Crop Production in Tropical Africa. Principles and Practice. ITA Publisher Netherlands, 25—28.Search in Google Scholar

27. Oridupa, O. A., Folasire, O. F., Owolabi, A. J., Aina, O., 2017: Effect of traditional treatment of diabetes mellitus with Xanthosoma sagittifolium on the male reproductive system of Alloxan-induced diabetic Wistar rats. Drug Res., 67, 6, 337—342. DOI: 10.1055/s-0043-103575.10.1055/s-0043-10357528288491Search in Google Scholar

28. Oridupa, O. A., Saba, A. B., 2012: Relative anti-inflammatory and analgesic activities of the whole fruit, fruit bark, pulp and seed of Lagenaria breviflora Roberty. J. Pharmacol. Toxicol., 7, 6, 288—297. DOI: 10.3923/jpt.2012.288.297.10.3923/jpt.2012.288.297Search in Google Scholar

29. Oyeyemi, M. O., Ubiogoro, O., 2005: Spermiogram and morphological characteristics in testicular and epididymal spermatozoa of Large White Boar in Nigeria. Int. J. Morphol., 23, 3, 235—239.10.4067/S0717-95022005000300008Search in Google Scholar

30. Rato, L., Alves, M. G., Socorro, S., Duarte, A. I., Cavaco, J. E., Oliveira, P. F., 2012: Metabolic regulation is important for spermatogenesis. Nat. Rev. Urol., 9, 330—338. DOI: 10. 1038/nrurol.2012.77.10.1038/nrurol.2012.7722549313Search in Google Scholar

31. Salimnejad, R., Sazegar, G., Borujeni, M. J. S., Mousavi, S. M., Salehi, F., Ghorbani, F., 2017: Protective effect of hydroalcoholic extract of Teucrium polium on diabetes-induced testicular damage and serum testosterone concentration. Int. J. Reprod. Biomed. (Yazd), 15, 4, 195—202.10.29252/ijrm.15.4.195Search in Google Scholar

32. Schoeller, E. L., Albanna, G., Frolova, A. I., Moley, K. H., 2012: Insulin rescues impaired spermatogenesis via the hypothalamic-pituitary-gonadal axis in Akita diabetic mice and restores male fertility. Diabetes, 61, 1869—1878. DOI: 10.2337/db11-1527.10.2337/db11-1527337964622522616Search in Google Scholar

33. Singh, S., Malini, T., Rengarajan, S., Balasubramanian, K., 2009: Impact of experimental diabetes and insulin replacement on epididymal secretory products and sperm maturation in albino rats. J. Cell Biochem., 108, 1094—101. DOI: 10.1002/jcb.22337.10.1002/jcb.2233719760637Search in Google Scholar

34. Soumya, D., Srilatha, B., 2011: Late stage complications of diabetes and insulin resistance. Diabetes Metab., 2, 9, 167. DOI: 10.4172/2155-6156.1000167.10.4172/2155-6156.1000167Search in Google Scholar

35. Waterhouse, B., Mitchell, A., 1998:Northern Australia Quarantine Strategy Weeds Target List. AQIS Miscellaneous Publication, Canberra, 29.Search in Google Scholar

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